EMAGE mouse gene expression spatial database

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EMAGE:14329

Rsrc1 arginine/serine-rich coiled-coil 1 ( MGI:1914130)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004168. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004168_01	euxassay_004168_02	euxassay_004168_03	euxassay_004168_04

euxassay_004168_05	euxassay_004168_06	euxassay_004168_07	euxassay_004168_08	euxassay_004168_09

euxassay_004168_10	euxassay_004168_11	euxassay_004168_12	euxassay_004168_13	euxassay_004168_14

euxassay_004168_15	euxassay_004168_16	euxassay_004168_17	euxassay_004168_18	euxassay_004168_19

euxassay_004168_20	euxassay_004168_21	euxassay_004168_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14329_wholemount_strong.wlz

14329_wholemount_moderate.wlz

14329_wholemount_weak.wlz

14329_wholemount_possible.wlz

14329_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14329_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7829
Entity Detected:	Rsrc1, arginine/serine-rich coiled-coil 1 ( MGI:1914130)
Sequence:	sense strand is shown >T7829 GTTCTGAGGACGCCGGTGGCGCCGTGGTTAGAAGAGGGGGTTCCAGGTCCCCACGTCGGGAGGTGCGAAC CCGAAGGTCGAGCCAGAAATGGGACGCCGGTCTTCAGACACTGAAGAGGAAAGCAGAAGCAAGAGGAAAA AGAAACACCGGAGGCGCTCGTCTTCAAGCAGCTCTTCAGACAGCAGGACATACAGCCGCAAGAAGGGCGG GAGGAGGCCGAGGTCCGACTCAAGGTCCTGGTCTAGAGACCGCCAGCTTCGCTCGCATTCTTATGAGAGA AGACGCAGGCGCAGATCAAGCAGCAGCTCTTCTTATGGCTCCAGAAGGAAAAGAAGTCGCAGTCGTTCCA GGGGCCGAGGGAAACCCTATAGAGTTCAAAGGTCTCGGTCAAAAAGCAGAACCAGAAGGTCCAGGTCAAG GCCTCGTCCACGCTCACATAGTAGAAGCAGTGAAAGATCCAGTCACAGAAGAACCCGTAGCAGGTCCAGG GACAGAGACAGACGTAAAGTCCGGGACAAAGAGAAAAGAGAAAAGGAGAAGGATAAAGGCAAAGACAAAG AAGTGCACAGCATCAAACGCGGGGATTCTGGAAACATCAAAGCTGGATTAGAACATCTGCCTCCAGCTGA GCAGGCCAAAGCCAGACTGCAATTGGTCCTTGAAGCTGCTGCAAAAGCTGATGAAGCATTAAAAGCCAAA GAAAGGAGTGAGGAAGAAGCAAAGAGAAGAAAAGAAGAAGACCAAGCCACCCTGGTAGAACAAGTCAAAA GAGTAAAGGAAATCGAAGCAATTGAAAGTGATTCTTTTGTTCAGCAGACATTCAGATCAAGTAAAGACGT CAAAAAGGCAGTAGAACCAAGTGAAGTGCAGCATGTGACTGCGGCATCAGGACCAGCATCCGCAGCTGCT GAGCCACCCAGCACTGGAAAGGAAATAGACCCTGACAGCATCCCCACGGCTATCAAGTACCAAGATGACA ATTCTCTAGCTCATCCAAATCTATTTATTGAAAAGGCCGAGGCTGAAGAAAAGTGGTTCAAGAGATTAAT CGCTCTCCGACAAGAAAGGCTGATGGGCAGTCCCGTGGCCTAAGCACGGACTTACGGTTGGATTGACAGT AACCTCGAAAACTTAGGTCGTAAAATTATATTACCAATATTAACTTTCACTCTTAAAAATAATTTAGCCA ATTACATAAAAAAGAAATCTCATTTCTTCCCTCATGTTCAACTAAATATGTGGTAATTTGAATCTAACAT TGTGAACAGTGAATCCGTTATAAAAGCTCTGAGTGCATGAAAACATATTTCACTATTAATAAAGTGAATA GTAAAAAATATTAGTGTTGAAATAAATGAACACTCAGTAGTGTTTCTCATTACAATTCTTACAAAGTCAT GGTTTTCACCAACTATTAGATAATTTTCTTTACGAGGGCTATGTTATTTAGTGTGAGAATGAGGTTTCAT CTCCTGTATTATGAAACCCTTCTTGAATCCCTTAGCTCCCCTTTCTTAAGGTTTTTAGGTATCATAAAAG GTCCGCTAAGAAGTCTAAGCAATGCCCATGTTAGATTTGAGGGCAACATGTTTTGTGTCAAGTGTGGACT CAGTCCTCTAACCCTAGGCCTTTGGAGGCTTAGGAGGGAAGGTCCCAATTCCAGTCTACACTACACAGCA AGTATTGGGATACCTCGGGCTAAATGTTCATTAAATAAAGGAATCGTTTTTGCTTAGAGATCGATTTGTA TCACGCGTTCTTTTGAAACCTGTAACCTGACTTCAGTCTTTACCTGTGGAAATCATATGCCCAATATTTT AGTCATCACTGTGCATTGCTGTGACAGAAGACCTGGCCTAACTTCAGGGAAAAGGTGTATTTTATTTCAT CTTATAGTTTGAGCAATGTCAGGCCATGGTCAGCTGGCTCCACTGCGTGGAGGCCTGTAGCAGGCAGAAA CACTGCAAGAGGCCACGTCCCAGGAAAGCCACTCACTAATGCCTGTCGGGAAGCAAAGAGACAGGCAGGG GCTAAGGCCATGGTACTTCAGGGACATACTCCAGCCACCTGCTTCTAGTCTGTCCCCTACCTCCTGGTAG CTCACTCAGCTCTAAGTGCATGGTGCAGTCCATTGATAAAGTCCTCATGCTCCGGTAACCGTCTCGGGAC ACCACCAGCACACGGCCAGGCTTTAAACATTTGAGCCTTTTGGAGACAGTTCATAGCCAGACCTTAATGC CCATGCTAGACTATGTGCCTGACCCAGTGTCATGATGGAAAAAGAATTTCTGAGAAATAAGAGCTCTTGC AGCACTCCTGCTAGGCAGTGTCTGCCCCCTCCTGAGGAAGCCGCTGTGTCGTGTGTAGAATGACATTCTC TAATAGACAAGCGTGGACATCTCTGAAGTCCATACCAAACATGCCAGAGGGAAGAGCTATGGGCCTTGCT GTACAAAATGGGATTTCAACCCCTGGCACAAACACTTTTTTCCCCATTTTTCTCTGCTTGCTTTTACACT GACTCTCTGAAGACTGGAGCTGAGGGCACAGCCATGGGAAGGCCCTGGGACATGAGGTCATTTAGATTCC ATCTTTTTTACACTTTAGAGCTACTGAAGTTCTCTTCTCTGTTAAGGATGAAAAGTGGCAGGACCTTACA CTCAGTTGCAAGTACTATAAAAACGTTTATGTTAAAATGTTTGTTTTCATGTAGATGTTTTCTTGATCTA AGCAAAGGCTATTTTCCTTGGCTCTACAAATAAACATGCCGAGAATGAAGGAGGCCCATGTGGTTGTTAA TGTGAACACGGCTATGCAGTAGGGATTTCGGAAGGGATTAATCTCAAATATTCTGCTTTTTAATTCTTTA TATTATTTGTAAACTGTGTGTTCCCATGCTATTAGGAAGTGTATATCTAGGAATGGATATACGCTATCAA TGGGTCATAGGGAGTCTTCCACACTTCTTCCATCTATACTGCAATAAAAATACTGTGATCTACTTTCTAG TATTCCTTCCCCCTAGGTTTCTCAATAACAGGTACACCAAATACAGATGCTTGAATTGTAACAAATTTGC ATCTTAAAGATAAACTTTTTGTGGAAAATAAAAAAAGTCAAGCTTTGTTTGGTTTTCATTTGTTTTAAGT AAGCAAATAAACTTGGTCCTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6334606 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6334606 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14332 same embryo
	EMAGE:14330 same embryo
	EMAGE:14328 same embryo
	EMAGE:14331 same embryo
	EurExpress:euxassay_004168 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source