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EMAGE:14426

Rnaseh2b ribonuclease H2, subunit B ( MGI:1914403)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14426 EMAGE:14426 EMAGE:14426 EMAGE:14426 EMAGE:14426
"Pseudo-wholemount" of euxassay_004223. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004223_01 euxassay_004223_02 euxassay_004223_03 euxassay_004223_04
EMAGE:14426 EMAGE:14426 EMAGE:14426 EMAGE:14426 EMAGE:14426
euxassay_004223_05 euxassay_004223_06 euxassay_004223_07 euxassay_004223_08 euxassay_004223_09
EMAGE:14426 EMAGE:14426 EMAGE:14426 EMAGE:14426 EMAGE:14426
euxassay_004223_10 euxassay_004223_11 euxassay_004223_12 euxassay_004223_13 euxassay_004223_14
EMAGE:14426 EMAGE:14426 EMAGE:14426 EMAGE:14426 EMAGE:14426
euxassay_004223_15 euxassay_004223_16 euxassay_004223_17 euxassay_004223_18 euxassay_004223_19
EMAGE:14426 EMAGE:14426 EMAGE:14426 EMAGE:14426 EMAGE:14426
euxassay_004223_20 euxassay_004223_21 euxassay_004223_22 euxassay_004223_23 euxassay_004223_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14426Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14426_wholemount_strong.wlz
14426_wholemount_moderate.wlz
14426_wholemount_weak.wlz
14426_wholemount_possible.wlz
14426_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14426_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16
4th ventricle
moderate moderate
regionalmoderate expression: see section 03 04 05
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15
liver
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
lung
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7901
Entity Detected:Rnaseh2b, ribonuclease H2, subunit B ( MGI:1914403)
Sequence:sense strand is shown

>T7901
CAGGGCGCGACCATGGCCGGAGGTCGGGACCGCGGGGACTTGGCGGCCAGGCAGCTAGTGTTCCTACTTC
CAGAACATTTAAAAGATGCCTCGAAGAAGAAGAAGAAGAGCAGCCTCCTGTTCGTAAAGCTGGCCAACCC
GCACTCAGGGGAAGGAGCCACATACTTAATTGATATGTGTCTTCAACAGCTGTTTGAAATAAAAGTTTTC
AAGGAAAAACACCATTCTTGGTTTATAAATCAATCAGTTCAATCAGGGGGCCTTCTCCACTTTGCCACAC
CCATGGATCCATTGTTCCTGCTCCTTCACTATCTCCTAAAGGCTGGCAAAGAGGGGAAGTATCAGCCCTT
GGACCAAGTCGTGGTCGATGACACGTTTCCAGATTGCACCTTGCTGCTGAGATTTCCTGAGCTTGAAAAG
TCACTTCGGCATGTGACAGAGGAAAAAGAAGTGAACAGCAAGAAGTACTATAAGTACAGCTCAGAGAAGA
CATTGAAGTGGCTGGAGAAGAAGGTCAACCAAACTGTGGTGGCACTAAAAGCTAATAATGTCAATGTTGG
AGCCCGGGTTCAGTCATCTGCATATTTCTCTGGTGGTCAGGTTTCCAGGGACAAGGAAGAGGATTATGTT
CGCTATGCCCATGGTCTGATCTCTGATTACATCCCTAAAGAACTGAGTGATGATTTATCCAAGTTCTTGA
AGCTTCCAGAACCTCCAGCTTCATTGCCAAACCCTCCATCAAAGAAACTAAAGTTATCAGATGAGCCTGT
AGAAGCCAAAGAAGATTACACTAAGTTTAACACTAAAGACTTGAAGACCGGCAAGAAAAATAGCAAAATG
ACTGCAGCTCAGAAGGCTTTGGCTAAAGTTGACAAAAGTGGAATGAAAAGTATCGATGCCTTTTTTGGTG
CAAAAAATAAAAAAACTGGAAAGATTTGAAGCATCGGAAATAAAATCCAGTGAAGCAGAAAAAAAAAAAA
AAAAA
Notes:The probe template was PCR amplified from IMAGE:6510494 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6510494 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14429 same embryo
 EMAGE:14427 same embryo
 EMAGE:14425 same embryo
 EMAGE:14428 same embryo
 EMAGE:14424 same embryo
 EurExpress:euxassay_004223 same experiment
 MGI:4827753 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS