EMAGE mouse gene expression spatial database

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EMAGE:14548

2310001A20Rik RIKEN cDNA 2310001A20 gene ( MGI:1919131)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005406. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005406_01	euxassay_005406_02	euxassay_005406_03	euxassay_005406_04

euxassay_005406_05	euxassay_005406_06	euxassay_005406_07	euxassay_005406_08	euxassay_005406_09

euxassay_005406_10	euxassay_005406_11	euxassay_005406_12	euxassay_005406_13	euxassay_005406_14

euxassay_005406_15	euxassay_005406_16	euxassay_005406_17	euxassay_005406_18	euxassay_005406_19

euxassay_005406_20	euxassay_005406_21	euxassay_005406_22	euxassay_005406_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14548_wholemount_strong.wlz

14548_wholemount_moderate.wlz

14548_wholemount_weak.wlz

14548_wholemount_possible.wlz

14548_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14548_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 13 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8722
Entity Detected:	2310001A20Rik, RIKEN cDNA 2310001A20 gene ( MGI:1919131)
Sequence:	sense strand is shown >T8722 TGAGTGTCAAGGCGCTGTTGGAGGTGTCAGTGGAGAGGCGGCAGAATGAGCGAAGCGGACGGGCTGCGGC AGCGTCGGCCCCTGCGGCCGCAGGTCGTCACGGACGATGGCCAGGTCCCGGAGGTCAAGGAGGGCAGTTC CTTTAGTGGCAGAGTCTTCAGAATGACCTTCTTGATGCTGGCTGTGTCCCTTGCCATTCCCCTACTTGGA GCCATGATGCTGTTGGAGTCCCCCATAGATCCTCAGAGTTTCAGCTTCAAAGAACCCCCTTTCATGTTTG GTGTTCTGCATCCAAATACGAAGTTGCGGCAAGCAGAAAGGCTATTTGAAAACCAACTTAGTGGACCAGA ATCCATAGTAAATATTGGGGATGTGCTGTTTACTGGTACAGCAGATGGCCGAGTTGTAAAACTTGAAAAT GGAGAAATAGAGACCATCGCTCGGTTCGGTTCAGGCCCTTGCAAAACCCGAGATGATGAACCTACCTGTG GGAGACCCCTGGGCATCCGGGCAGGGCCCAATGGGACTCTTTTTGTGGTTGATGCGTACAAGGGACTGTT CGAAGTAAATCCTCAGAAACGTTCAGTGAAACTGCTGCTATCCTCTGAGACGCCCATTGAGGGCAAGAAG ATGTCCTTTGTGAATGACCTCACTGTTACTCGGGATGGAAGGAAGATTTATTTCACGGATTCCAGCAGCA AGTGGCAGAGACGAGACTACCTGCTTCTGGTGATGGAGGCCACTGATGATGGGCGCCTGTTAGAGTATGA TACTGTGACGAAAGAAGTGAAGGTTTTGTTGGACCAGTTGCAGTTCCCTAATGGAGTTCAGCTCTCTCCT GAGGAAGACTTTGTCCTAGTGGCAGAGACAACTATGGCCAGGATACGAAGAGTCTATGTGTCTGGCCTGA TGAAAGGAGGGGCAGATATGTTTGTGGAGAACATGCCTGGATTTCCTGACAATATCCGGCCTAGCAGCTC TGGCGGATACTGGGTTGCTGCTGCAACCATTCGTGCTAACCCTGGGTTTTCCATGTTGGATTTCTTATCT GACAAGCCTTTTATTAAGAGAATGATTTTTAAGATGTTCAGTCAGGAGACAGTGATGAAGTTTGTGCCAC GATATAGCCTGGTCCTAGAAGTCAGTGACAGTGGTGCCTTCCGGAGAAGCCTGCATGATCCTGATGGACA GGTGGTTACCTATGTGAGTGAGGCGCATGAACATGATGGATACCTGTACCTGGGCTCCTTCAGATCTCCC TTCATCTGCAGACTCAGTCTCCAGTCTATTTAGCCAGCCCAGCAGCTGCCCCCACTGTGCATGCCAGAAA TCATCACACTCAGTCACATAGGTCTGGAAGGAGCCATGGACATATAGCTCTGTCCCTGTGTTCCTGCTAG TCCTTGGAAGTGTGGGAGTAGCTGCTGTAACCTGGAGAAGGTATGAACCTTACCCATACTTTCTCCCAAG ACGTGCCTCTCACCTGGACTTGATGTCATTTTTAGAGGGAAATAGTGTATCTGCTATGGGGAAGACAAAT CATGGAAAGCCATTTCTCTTTAAAAAAACATGCTAAGTACAAGAATTCTCTAGTACCTGGAAAATTTAAT ACACCTGTGACAGGGCTCAGGGTTTGATGATTAGAGCAGGGGATTAGCTGTGCAGGGACCTTTGTATTGC CATTGGCTCTATCTGACACTTGTATCTTCATTCTAAAGGGGGTCATGCTTTTGGGGGTGGTATTGCGGTA TGCCATGGAAGTGGTAATGCATGTGTTGAGGCAGGGATTATTCAAGCTGAAACTTGTTCTCAGGGGCCGT GTGAGATGTGTGAGAATACCTGGACTTCTGGTATTTCTCCACAGAAAAGGTGTGCTCTCCCACCCTCTAC AAGCCTCTTTCCATGCCAGAGGGTTTTCAAGACTCCTGTTTTAGTGGCCAGGAGGATTTCATTGGGTTTC TTTCTGACTGCCTTCTCCACTGGAATTGGGTGACCCAGTGAGTTTGTACCTGCACAGGGGTGCTTTAGAA ATGCCCTTTTATGGCTTGATTCCTTTTAGACCTACAGATGTGATTAGTGAACTGTTGATGTAATGTTTGT GCTAATGTAATAAGCTCAAAGTTGCTTATTTCTTGGCAAGTATGTTTATACACTGGTCTTAATTTTGATA ATAATAAATCATATTAAACCCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6403495 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6403495 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14552 same embryo
	EMAGE:14550 same embryo
	EMAGE:14551 same embryo
	EMAGE:14549 same embryo
	EMAGE:14547 same embryo
	EurExpress:euxassay_005406 same experiment
	MGI:4822656 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source