EMAGE mouse gene expression spatial database

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EMAGE:14551

Nif3l1 Ngg1 interacting factor 3-like 1 (S. pombe) ( MGI:1929485)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005419. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005419_01	euxassay_005419_02	euxassay_005419_03	euxassay_005419_04

euxassay_005419_05	euxassay_005419_06	euxassay_005419_07	euxassay_005419_08	euxassay_005419_09

euxassay_005419_10	euxassay_005419_11	euxassay_005419_12	euxassay_005419_13	euxassay_005419_14

euxassay_005419_15	euxassay_005419_16	euxassay_005419_17	euxassay_005419_18	euxassay_005419_19

euxassay_005419_20	euxassay_005419_21	euxassay_005419_22	euxassay_005419_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14551_wholemount_strong.wlz

14551_wholemount_moderate.wlz

14551_wholemount_weak.wlz

14551_wholemount_possible.wlz

14551_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14551_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8724
Entity Detected:	Nif3l1, Ngg1 interacting factor 3-like 1 (S. pombe) ( MGI:1929485)
Sequence:	sense strand is shown >T8724 GCGACTGGCACCCTTCTCCCTGGTTTCCGTATGCTGTCATCGGCACACCTAGTCCCTACAAGTGTGCAAC GTGCCCAGTCGTGGATCTGCAGGTCTTCCCGTTCCTTCATGGATCTGAAGGCTCTTCTCTCGTCCTTGAA TGACTTTGCATCCCTCTCATTTGCTGAGAGCTGGGACAATGTGGGATTACTGGTGGAGCCAAGCCCACCC CATACTGTAAATACACTCTTCCTGACCAATGACCTGACGGAGGAGGTCATGGACGAGGCTCTGCAAAAGA AGGCAGACTTCATTCTCTCCTACCATCCACCTATTTTCCGGCCCATGAAGCACATAACTTGGAAAACCTG GAAGGAGTGTCTCGTGATCCGGGCTCTGGAGAACAGAGTCGCTGTCTACTCTCCCCACACAGCCTATGAT GCGGCACCCCAGGGAGTCAACAGCTGGTTGGCCAAAGGGCTTGGAACTTGCACTACCAGGCCCATCCACC CTTCCAGAGCTCCAGACTACCCCACAGAGGGAGCTCACCGACTAGAATTCAGTGTGAACCGCAGCCAAGA CCTGGACAAAGTCATGTCTACACTGAGAGGGGTTGGAGGTGTCTCTGTCACTTCTTTTCCTGCCAGGTGT GATGGTGAAGAGCAAACCCGGATCAGCCTGAATTGTACTCAGAAGACTTTGATGCAGGTGCTAGCCTTTC TTTCCCAGGACAGACAACTTTATCAGAAAACTGAAATTCTTTCATTGGAGAAGCCTTTGCTTCTGCATAC TGGAATGGGACGGTTGTGCACACTGGATGAATCTGTCTCCCTGGCAATAATGATAGAGCGAATCAAAACA CACCTAAAGCTGTCGCATCTTCGCTTAGCTCTTGGAGTGGGGAGAACATTAGAGTCCCAAGTCAAAGTTG TGGCCCTGTGTGCTGGTTCTGGGGGCAGTGTTCTACAAGGAGTGGAGGCCGACCTTTACCTCACAGGTGA AATGTCCCACCATGATGTTCTGGATGCTGCTTCCAAAGGGATCAATGTCATCCTTTGTGAACACAGCAAC ACTGAAAGAGGCTTCCTTTCTGAGCTTCAAGAAATGCTGGGTGTTCACTTTGAGAATAAGATTAACATTA TCCTGTCTGAGACAGACAGGGACCCTCTCCGTGTGGTTTAAATGCAAGGACGTCAAGGACACCATGATGA CAGAAGCCACAGATCACCTGGATTGAAACGGCTGTGCAGGAGTTGGTGTTCCACCCTCTGCAGAGCTGTA TTGGACTTCTCATTTCAGTTCACGACTCTGGCTCAACAAAAGCCATAGCTCATTCGGTAAAACTGTCTGT AAAGAGTACATTAAAGGCAAACCTTCTCTAATTTTTGTTTTGTTTCTTGGTGGTGCAGGGATCAAACTGG ACACTGTTTACCACTCTAGTTGGCAGCATGGACTTGAGAGTAGGGCCTAAAAATGAGGTGAGCTGATGTC TCACGCAATAGCCAAGTTTATTCAGAGCCATCGGACAATTTACACTCTGAGGGCTAAGAGTCAACTGAAT AAAGGCACAATCACAAGGACAAGCCTCATGTAGCAAAACCATGGTTTCCATGGAGGTGTATGAACAATAA GAATAGCCAGCCAACTGTAAGGAATGATCTAAAGTCACCTCACTCCTATCTGCTTGCTCCACTGGGGAAG GGCACAGAAGCACATCCCAAGAACAATTTTGTGTTTTTGAAGAAACTGTTTTCTCACAAACACTCAGAAG TCCCTCACAGGACTGGACTATTCTTGGACTATGTTCTAGCTCTATGAATGGAAGGCAAGTGTTCTACCAC TGAACTCCAAACTCCTCCTAATTAATGGTGTTAAGTGTTCAAAATATGACTGATTACTACCCAGCATTTT CTGCAGTCACTGTACAAGCCAGACCCAGAAAGAAACTGGGGTGCCCGGGGGGAAGGGGTGGCTGGAGAGA TGGCTCAGCAGTTAAGGGCACTGACTGCTCTTCCATAGGTCCTGAGTTCAATTCCCAGCAACCACATGTA ATGGGATCCAATGCCCTCTTCTAGTGTGTGTAAAGACAGTTACAATGTATTCACATACATTAAATAAACC TTAAAAAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6404016 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6404016 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14552 same embryo
	EMAGE:14548 same embryo
	EMAGE:14550 same embryo
	EMAGE:14549 same embryo
	EMAGE:14547 same embryo
	EurExpress:euxassay_005419 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source