EMAGE mouse gene expression spatial database

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EMAGE:14550

Cggbp1 CGG triplet repeat binding protein 1 ( MGI:2146370)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005412. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005412_01	euxassay_005412_02	euxassay_005412_03	euxassay_005412_04

euxassay_005412_05	euxassay_005412_06	euxassay_005412_07	euxassay_005412_08	euxassay_005412_09

euxassay_005412_10	euxassay_005412_11	euxassay_005412_12	euxassay_005412_13	euxassay_005412_14

euxassay_005412_15	euxassay_005412_16	euxassay_005412_17	euxassay_005412_18	euxassay_005412_19

euxassay_005412_20	euxassay_005412_21	euxassay_005412_22	euxassay_005412_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14550_wholemount_strong.wlz

14550_wholemount_moderate.wlz

14550_wholemount_weak.wlz

14550_wholemount_possible.wlz

14550_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14550_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8764
Entity Detected:	Cggbp1, CGG triplet repeat binding protein 1 ( MGI:2146370)
Sequence:	sense strand is shown >T8764 GCAGCGGCGGAAGCAGCGGCGGCCGCAACGGGATCTCCTTCATCCCGTTGCTCTGCCCTTTCTTCCTCCT CTCTTCCTTCCTGGCGAGGGCTCGTGCACTTCCCCTAACGGCGGCCCGCTCCTACGGTCCCGAAGAGCTG GGAAGGCGGAAGTAAAGACATGACTTCCCGGCGCCACCGGGAAAATAGGGTGCAAGCCCAGGAACTGTTT CCCCACCACCACCTGTTGAAAAACTGAGTCGAAGGCGTCTCCGGGGTCTGAACGAACGCGAAGTCGCTCG GATTGGATGCGTCTGTGGTTTTGCATCCAGAAAACTGTCACTACTTAATATCAAGGAAAAACAAACAAAC AAACAAAAACCCGACTGGAAATTAAGCTGAAGAACCTTATTCAGAGACAAAATGGAACGATTTGTTGTAA CAGCACCACCTGCTCGAAATCGTTCTAAGACTGCTTTGTACGTAACCCCTCTGGATCGAGTCACTGAATT TGGAGGTGAGCTTCATGAGGATGGAGGCAAACTCTTCTGCACCTCTTGCAATGTGGTGCTGAATCATGTT CGCAAGTCTGCCATTAGTGACCACCTCAAGTCAAAGACTCATACAAAGAGGAAGGCAGAATTTGAAGAGC AAAATGTGAGAAAGAAGCAGAGGCCCCTGACTGCATCGCTTCAGTGCAACAGTCCTGCGCAAACAGAGAA AGCCAGTGTTATCCAGGACTTTGTGAAAATGTGCCTGGAAGCCAACATCCCCCTGGAGAAGGCCGATCAT CCCGCAGTGCGAGCGTTCCTGTCCCGGCATGTGAAGAACGGAGGGTCCATACCTAAGTCAGACCAGCTGA GGCGAGCATATCTGCCTGATGGCTACGAGAATGAAAATCAGCTCCTCAGCTCACAAGATTGTTGACGAGG CAGTTAATGACCATTGTGATCAAGATAAATAATGTGGAGTATTAAAGTTAATGTGTTGATTGTGTGGTTC ATTTTTATATTTATTTTAAATCATGTGATACAGAATAGTTTTGCAATGTGTATATAGTTTTAGGCAAAAG AAAGAAACCTCACTGCAAAACTTACTGCTAATTTTAAGTCACCGATGGTGTAAAGTAAAACTTACCTATG GAGTGTATTAGGCTATCTGAAACCCGGTGGTTATCGTTAAAACTCATGGATGTTCTCCTGAAATGTTTGT GCATGGAAGAACTTCCTTGCATTGTAGCCCTGTTGCCATGTATGGTTTTTGTTTTTTCTTGTGAGGTTAC GTAATGATTTGGATTGAGAACTAGCCTAGAAAACTGAAGCTGCTGCCAGGCTATACCTCTCACAGGACCT TAAAGAAGCTCACATTCTTTTCAAAGTGGAATAGTAGGCCGGAAATGTTCTTAGAGCTTGAGATCTCTAG GAGAAATGACTGTTCAAATTACATTCTACTTGTATGTGAAATAATTGCTTAACCTTCACTTCCTTGTGGT CGCTTTTAAAGACATATTTAGTAGCAGCTTCAAGTGACTAAAAGACTAAAATCTCTCTTAGTGTCTTTGA CAAAAGCCATGGGGGATTTTGCAGTGATGAGATTTTAGTCTTCAACTATGAACAAAAATTTTCAAACCAT AGTTTTCATGTTAAGTAGCATCCTGGATTTGCAAACTTTTTTATGTCTTGGTATTAAGACTATTAAGACA GTCTTGTAGTCTTAATTTAAATTTAAATACACTGTGGTTCTGTTTTCTCTTTTTACAGGGAATTGGTTAC TTTTTGGCTGACAACAAATAAGAAAATTGCTTTCTATACATTAATTTTTCCATAAGATTTTATAAGAGTT TATTTAGGTAAATAGACTAGAAATGAGTTTTAAAAGTTTCACCTTTACTGCTTTGCCTCTGGTGATGCAG GTTTTAAAACTTGGATGCTAAACAGTGCAGAAAACGAGGCAAGAACTTTATAGTAAAATAAAGCAGGATG CTGAGTCAGGAAATAATCAGCTCTGTCAAAGGTCAACCGTGTTTAGGTTTTCTACACTTCACTCTTCTGT AAGCTTATTAGGAGTCATTTAGCTCAGAGAAGTCCTCTTCATCAAATCCGGATTATATTTTTTAAAAGTT TAGTAACTGAAGACTTAGATTAGAGCTTTAATATTAAAAAAAAATCAAATAGTTGAAAACATTGAAGTTA TATTTCTATTCTAAATCAGTGGCAAGATGCCCTGATTGTGACTTGGAGTTATCTTTGAGGTCATTGTGCC ACAAATTTCCATTAAACAAAGCCAAAGTTAATAGAAAATAATTTGTGGGTTTTGTGCTTTGATTTTTTTT TTCCCCCGTCACAGGGTGTTTCTACATAGCCCTGGCAGTCCTGGAACTCACTAGAGGCTAGCCTCAAGAC CCACCTGCCTCTTTCCCTCGTGAGTGCTGGGATTAATGGCGTGTACTATCACGCCCAGCAATAATTTGTG TTTTTAAGAACATCTTGTCTTATGGGTCTTACAGATTTAACATTCCCAATTCAGGTTGTCAAAGTGACCA CTTGTTTTTATTTTAAAAGCCTGCTATGGAATTTAAAAAAAAATAGTTCTCAAAAATTGCAGTTTCTATT TTCTCACGTTTGGTTATATTCATGTAGCACATAAATGCCTAGACTAGTTGTTTACTAACTGCAATTGGTA AAACACAGACCAGCAGCAAAGTAAACAATGATTGCTTCCACTTCCTTAAGGAATTTGCGGGCAACCATGT TTAAATTGCCTAAGATGGTAGGATATCTAAGCAACTAAAATAATTGAGTTTTGTACCACTATTTTCAGAA GAAAAACGATCATTTAATTTTCCAAGATTCCTTGTGATTCGGGCTATTCCCTATTGTCTGGGACTATATG CGCTGTCAGTCAACATGGCAGAACAGCTTCTAGCAGTCGAGTAACATGTTGGACGGTGCACTAGGAATAT AATTCACCTTTGCCTTTCTTACAGTTATTACTGATTTCACCGTTACTTAGTTCCTCTAGAACTGTTTTTC CAGGCTTATTTTTGAATCTCTAGGGGCAGAGTGCATTCTGTTAGGGTTGTATCATGTCCTTAGAATGAGG TTTCCGTCCCCTTGAGGGAAAGTTAGTAGTAATGTTTCTTCACCGATTATGAACATTTGTTTCTTGGAAC TGGTTAAATTTGTCTCGTAAATAATAACAGTGGTTATAGTAGCCTGTGCAAACTGGTAAAGATTTGCAAA ACTATGATTGGCATGTTAGATAACAAATGAGAAGTGTCTAAGGCGCGTGCTATGTGAGAGAGGCAAAGTG AACTTATGTACAGAAGCTTTCAATCCTCCTTATATAGTGGTAGTTGATGCTTTACTATTGGACCAATAGC AGCTCCTGTTAATTAGTTGGATGCATTATCACAAGCTTTGTAATGTTTCATTTATAAACTGCCTTCAGCA CATGCGTACCTGTTAATGTTTTCTCAGTGTCGTAGGATGTCTTCCAGACTCTCACTGTATCCTGCCAGTA AATAGTTCTAGCTTGTTGAATTTCCTGCTTATTTTTCATTATCTGTGGCATATAGCAAGCCTAAAAGATA ATTGTAATCATTTTTTTTTCTTTTACAGAGTGAGAAGTTAGATTGTGTATGAAAGCCTATAGTTATTTTA TCCATTATCTTTGATTAAGGCTTTCTAATATTATTTAGTAAGTGTTCCTTCCAGTTAATGCCTGTGGCCC TACTGAATTTTAAGGGAACAGACAACTTTTAAGAAATAAACCTTACTGTTTTATACAGTAAGTTTGTCTG ATTGGGAATGTTCTGAAGTTGGACATACAATTGGCCAGATTCCCAAGCTAAAACCTTGGCCTGAAAGGCT GTGTACATGAATGTTAACAAGTACAGGAAAGCAAATTAAACTTAAGGTGACCTAAAACAAAGGCTAGAAA CAAGTTTTCATTGGATTCTTAGTCTTTGTAAGGGCTTTCTGTTTGACTTACCTCAACTGCCATTGAGTGA GGGTAAATCACCTTAAAATGACTGATTTACCTGGAAGGGTAGTTGTAGTTTCCATCCTTAGCTTGCTTTT GCTTTGGTCGGCACTGATGAAATGTGAAGTGGCTATTCTAGACCTTGGTTGATCTCAGCCCCTACTCCCA GACTGCTGAGGTAACTACAGTCCGTTACCTAGTGCTATTCTCCTTGTATCCAGAACACTATTAAAATTTT ATTATGTTAATTTTCGTACTCCTGTGCTTCTTTTTTACTTTTGCTCATGCACGTATTTTAGTATGAATGT GTTGCTTTTTTAAAAGTTTGTGATATTTAGAAATAAATTTCAAAAAAGTTAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6405954 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6405954 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14552 same embryo
	EMAGE:14548 same embryo
	EMAGE:14551 same embryo
	EMAGE:14549 same embryo
	EMAGE:14547 same embryo
	EurExpress:euxassay_005412 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source