EMAGE mouse gene expression spatial database

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EMAGE:14605

Cst13 cystatin 13 ( MGI:1916544)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005464. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005464_01	euxassay_005464_02	euxassay_005464_03	euxassay_005464_04

euxassay_005464_05	euxassay_005464_06	euxassay_005464_07	euxassay_005464_08	euxassay_005464_09

euxassay_005464_10	euxassay_005464_11	euxassay_005464_12	euxassay_005464_13	euxassay_005464_14

euxassay_005464_15	euxassay_005464_16	euxassay_005464_17	euxassay_005464_18	euxassay_005464_19

euxassay_005464_20	euxassay_005464_21	euxassay_005464_22	euxassay_005464_23	euxassay_005464_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14605_wholemount_strong.wlz

14605_wholemount_moderate.wlz

14605_wholemount_weak.wlz

14605_wholemount_possible.wlz

14605_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14605_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9892
Entity Detected:	Cst13, cystatin 13 ( MGI:1916544)
Sequence:	sense strand is shown >T9892 GAGGCCAGAAGCACCTGGGGCTCAGATCCCCACAGATGCCTATCTGGAAGCAGGAGCATGAACTTGGTAT GCTGGTGATCAAGAGCAACCTGAGGCTAGAGGCCATTTAATCTTAGTCTTCACTACAGATAAAGCCGTGT CAGGTGTTGAAGAGAAAGGATCCATTGACCTGCAGAGAAAGCAGGGGCCTCACTGGCTACATTGACACCA GCTCTGGAAAGAAAATAGGAACTTGGTATGTTCCTTGAATGAAGAAGCACCATGGCCAGATTCTTACAGA CCCTGCTGTTCCTGGTGATCATGGTGGAGTTTGTATCTAGAAGAGTCGAAGCCTGGGGCTCCCCACAGAT TGTGAGGCCATTCGAAGACATCCCCAAATCCTATGTCTATGTGCAGCATGCACTCTGGTATGCCATGAAA GAATATAACAAGGCCAGCAATGACCTCTACAACTTCAGGGTGGTGGATATCCTAAAATCTCAGGAGCAGA TCACAGACAGTCTGGAGTATTATCTTGAAGTAAACATTGCCCGAACAATGTGCAAGAAGATTGCAGGAGA TAATGAAAACTGCTTGTTTCAACAGGATCCTAAAATGAAAAAGATGGTGTTTTGCATTTTTATTGTTAGC TCCAAACCATGGAAGTTTGAACTTAAAATGCTGAAGAAGCAATGCAAAGATATCTAATCAGCATTCGGGA CACCTTGCCACTCTTACTTTTCTTTTAACTGAATAAAGATGCTTGTAAGATAGTGGACCCCCTCAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6774445 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6774445 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14607 same embryo
	EMAGE:14609 same embryo
	EMAGE:14606 same embryo
	EMAGE:14608 same embryo
	EMAGE:14604 same embryo
	EurExpress:euxassay_005464 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source