EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14637

Tfrc transferrin receptor ( MGI:98822)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005557. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005557_01	euxassay_005557_02	euxassay_005557_03	euxassay_005557_04

euxassay_005557_05	euxassay_005557_06	euxassay_005557_07	euxassay_005557_08	euxassay_005557_09

euxassay_005557_10	euxassay_005557_11	euxassay_005557_12	euxassay_005557_13	euxassay_005557_14

euxassay_005557_15	euxassay_005557_16	euxassay_005557_17	euxassay_005557_18	euxassay_005557_19

euxassay_005557_20	euxassay_005557_21	euxassay_005557_22	euxassay_005557_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14637_wholemount_strong.wlz

14637_wholemount_moderate.wlz

14637_wholemount_weak.wlz

14637_wholemount_possible.wlz

14637_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14637_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 06 07 08 09 16 17 18 19

diencephalon meninges

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16

telencephalon meninges

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hindbrain meninges

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16

midbrain meninges

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14

spinal cord meninges

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09

stomach

weak

regional

weak expression: see section 04 05 06 07 08 09

midgut

moderate

regional

moderate expression: see section 13 14 17 18 19 weak expression: see section 12 15 16

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09

liver right lobe

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19 20 21 22 23

left lung

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10

right lung

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8762
Entity Detected:	Tfrc, transferrin receptor ( MGI:98822)
Sequence:	sense strand is shown >T8762 CGGCACGAGGGTAGAGGCGCTTCCTAGTACTCCCTTGTAGCAGCTGAGAATGATGGATCAAGCCAGATCA GCATTCTCTAACTTGTTTGGTGGGGAACCATTGTCATACACCCGGTTTAGCCTTGCTCGGCAAGTAGATG GAGATAACAGTCATGTGGAGATGAAACTGGCTGCAGATGAAGAAGAAAATGCCGACAATAACATGAAGGC TAGTGTCAGAAAACCCAAGAGGTTTAATGGAAGACTCTGCTTTGCAGCTATTGCACTAGTCATTTTCTTC TTGATTGGATTCATGAGTGGCTACCTGGGCTATTGTAAGCGTGTAGAACAAAAAGAGGAGTGTGTGAAAC TGGCTGAAACGGAGGAGACAGACAAGTCAGAAACCATGGAAACAGAGGATGTTCCTACATCATCTCGCTT ATATTGGGCAGACCTCAAAACACTGTTGTCAGAGAAGTTGAACTCCATAGAGTTTGCTGACACCATCAAG CAGCTGAGCCAGAATACATACACTCCTCGTGAGGCTGGATCTCAAAAAGATGAAAGTCTTGCCTATTATA TTGAAAATCAGTTCCATGAATTTAAATTCAGCAAAGTCTGGCGAGATGAACACTATGTGAAGATTCAAGT GAAAAGCAGCATTGGTCAAAACATGGTGACCATAGTGCAGTCAAATGGTAACTTAGACCCAGTGGAGTCT CCCGAGGGTTATGTGGCATTCAGTAAACCTACAGAAGTTTCTGGTAAACTGGTCCATGCTAATTTTGGCA CTAAAAAGGACTTTGAAGAACTAAGTTATTCTGTGAATGGATCTTTAGTGATTGTTAGAGCAGGGGAAAT TACTTTTGCAGAAAAGGTTGCAAATGCCCAAAGCTTTAATGCAATTGGTGTCCTCATATACATGGACAAG AATAAATTCCCCGTTGTTGAGGCAGACCTTGCACTCTTTGGACATGCTCATCTAGGAACTGGTGATCCAT ACACACCTGGCTTTCCTTCTTTCAATCATACTCAGTTTCCGCCATCTCAGTCATCAGGGTTGCCTAATAT ACCTGTGCAAACAATCTCAAGAGCTGCTGCAGAAAAGCTATTTGGAAAAATGGAAGGAAGCTGTCCTGCT AGATGGAACATAGATTCTTCATGTAAGCTGGAACTTTCACAGAATCAAAATGTGAAGCTCATTGTGAAAA ACGTACTGAAAGAAAGAAGAATACTTAACATCTTTGGAGTTATTAAAGGTTATGAGGAACCAGACCGTTA TGTTGTAGTAGGAGCCCAGAGAGACGCTTTGGGTGCTGGTGTTGCGGCGAAGTCCAGTGTGGGAACAGGT CTTCTGTTGAAACTTGCCCAAGTATTCTCAGATATGATTTCAAAAGATGGATTTAGACCCAGCAGAAGTA TAATCTTTGCCAGCTGGACTGCAGGCGACTTTGGAGCTGTTGGTGCCACTGAGTGGTTGGAGGGATACCT TTCATCTTTGCATTTAAAAGCTTTCACTTATATTAATTTGGATAAAGTTGTCCTTGGTACTAGTAACTTC AAAGTTTCTGCCAGCCCCTTATTATATACACTTATGGGAAAGATAATGCAAGATGTAAAGCATCCAGTTG ATGGAAAATCTCTATATAGAGACAGCAATTGGATTAGCAAAGTTGAGAAACTTTCCTTTGACAATGCTGC ATATCCTTTCCTTGCATATTCTGGAATCCCAGCAGTTTCTTTTTGTTTTTGTGAGGATGCAGACTATCCT TATTTGGGCACTAGATTGGATACCTATGAGGCATTGACTCAGAAAGTTCCTCAGCTCAACCAAATGGTTC GTACAGCAGCGGAAGTGGCTGGTCAGCTCATTATTAAACTTACCCATGACGTTGAATTGAACCTGGACTA TGAGATGTATAACAGCAAACTACTGTCATTTATGAAGGATCTGAACCAGTTCAAAACAGATATCAGGGAT ATGGGTCTAAGTCTACAGTGGCTGTATTCCGCTCGTGGAGACTACTTCCGTGCTACTTCTAGACTAACAA CTGATTTTCATAATGCTGAGAAAACAAACAGATTTGTCATGAGGGAAATCAATGATCGTATTATGAAAGT GGAGTATCACTTCCTGTCGCCCTATGTATCTCCAAGAGAGTCTCCTTTCCGACATATCTTCTGGGGCTCT GGCTCTCACACTCTCTCAGCTTTAGTGGAGAACTTGAAGCTTCGTCAAAAAAATATTACTGCTTTTAATG AAACCCTCTTCAGAAACCAGTTGGCCCTGGCTACTTGGACTATTCAGGGAGTCGCAAATGCCCTCTCTGG TGACATTTGGAATATTGACAATGAGTTTTAAATGTAACATGCATAATTAAATAAGAGCAGGGTAGTCTGT TTCTAGACTTGTGTTGGTTGTGCTAAATTTTCATTAGAGCTCTAAAATCTAATGTTAAAATTCTACCCAA TCATCTAATGTCTTAGGCAGCAGCTTTTAGTGCGGGGTTGGACCTACACTTCAAGTACAATGGATAACAC TTTCCATGTTTGTGATATCTTCTCAGATTATCTTTAAAATTTTTGAGTCCTTTGTAATAACTTCCTCTTT GCTTCATGGTCATGAAAATGTCAGAACCAGTTGTAAGAACAATGCTATATGTCCTGAGGGCATTAACTAG TGTCTTTTGAGGTAGGAGAAAGAGTGTGCTTGTGGAGGGTCAACGTGGTAGTTGGGTAGTTGGAGATTGC CCCGTCTCCATCTGATCTTCCATTGAGCTGTATGCCCTTAAGGATGTAGCTGGTTTGCATTTCCCTAAAT TAGACAGTAACTTTCAGAAGAGATGGAACTTGTTTTCTTGCCAGCAAGGTTGAAAATAGGTCCTTAGCTG GACCAATTTGTCTGTTTACAGGAATAAGTAAGGCCTTACTGGTTAACCTTGGTGTTCTTTATGATGAGAC CAGAAGCCAAAGACCTCAAGTTTTCTCTCCTCTTGGCATCTGCCCTATAGTCTTTAGTTCTTTGTTTTTA TTTTGTTTTTCTTTTTTTCCCAACATTTTCTGAAAAAGAACAAGTTTTAGACTCAATTTGTCAGACTTGA AAAGAACACACTGCCAAGTTTTGGCCAAAGTGTTAGTCTTCAGGAAAGCTTTCTATCATTTTGGCACTGA GATATTTATTGTTTATTTATCAGTGACAGAGTTCACTATAAATAGTGTTGTTTTTTTTTTATATATAGAA GATAATTATCGGAAGCAGTGCCTTCCATAATTATGACAGTTATACTGTCGTTTTCTTTTAATAAAAGCAG CATCTGCTAATGAGACCCACAGATACTGGAAGTTTTGCACTTATGGTCAGCACTTGCAACTTTAGGAAGG AAGAATGCCACAATCCAAATAATATGAGCTAGAAGAGGATTGGGTTAAATAAGAGATTCCTAATTGAGTT GGGGAAAAAAATGATAGTTTTCCTAAGTGCAGTGAGTTGTGGCCAAGTTAAATGTCATTTAAAGGCTATG GTAGTACTTACATCTACAAAATTTTACAGCTCAATTTATTCAAGATGTAACTCAAAATCAATTTTGCAAA ATTTCCAGTACCTTTGTCACAAACTTAACTCACATTATCGGGAGCAGTGTCTTCCATAATGCATAAAGAA CAAGGTAGTTTTTGCCTACCACAGTGTCTATATCGGAGACAGTGATCTCCATATGTTACACTAAGGGTGT ACGTAATTATCGGGAACAGTGTTTCCCATAATTTTCTTCATGCAATGACATCTTCAGAGCTTGAAGATCG TTAGTATCTAACATGAATTCCTAACTTCCTGTAGCTCCTTAGTTTTAGTTGCAAAAAACATTTGTGGTCA TTGAGCATTTGGTGGGTAAAATCAACTGCTGTAAAATCATTACTTCCAAGATCCTTTTTTAAAATGTGGG TATTTTGTTATCTCTGGTTTATGGAATAAAAGCATACGTTTATAATGTTTGCTGGTGACAAAGGAATTTA ACTATCTCCCTTTATTTGCATGTTACATGTTATTTCCTAAATGTAGTTCTTAGAAGTTTGAATTCATTTT TGGTTTTTGGGAGGGTAGAAAGTAGAGAATAATGGGTGTTGGGAAGACAAAGCTTGCAAAAGGATCTTTA TGTACCTGCTCATCACTATGGTGGCTATGAATTATGTAGGTAATGAGCAAGATCACATTAACAAAGACTA GTTAACCATCATTACGCATCTAATCAGTTTTGCCATGGGGTCAGTTCAAAGCTGCCACCTGAGAATGTCA CTAGGCTCTCAGGGTCTTGGCACCACTCGCCCAAGTTATATCCACCAGATTATTTTGAGCCTCACTGAGT TGTTTTGCTACTCAGTCCCTTCAGTCTTCACAGTGTTTCTCTTGTACCAGTATGAGTATCACCGTGCATA ATGGGATTCCAAGTCTTGAATGAAGGCATGCATTTTACATAGACGTCTCTGTGAAATAACCTTTGTTGTA AGTGGTGTTTCCAGTCAAAGCAGTAAGTTGGTAAGGTTTAGTCTTGGGTGAAATGTGGATCTGAGTAATA AGTAGCTTCTGCTACTTATCAGGTTATTGTTGGTTAAAATGTAGATTTTGAAGATAAAAATAGATCTTTT GTCATGACTCACTGCTAGAATTTGCATGACCTTTACTGTGTTAGCTTTTGAATGCCTTTTGGTTTGGACT TGCCAACCTATACTGTCTTTGCTGAAATGCCCTTATAAATGTATACAGCTGGTATGTAACAATGTGAAGA TTCCTTACCTGACTTAATAAAATACTTCGATAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6406097 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6406097 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14638 same embryo
	EMAGE:14641 same embryo
	EMAGE:14640 same embryo
	EMAGE:14639 same embryo
	EMAGE:14636 same embryo
	EurExpress:euxassay_005557 same experiment
	MGI:4828675 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source