EMAGE mouse gene expression spatial database

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EMAGE:14638

Dync1li2 dynein, cytoplasmic 1 light intermediate chain 2 ( MGI:107738)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005538. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005538_01	euxassay_005538_02	euxassay_005538_03	euxassay_005538_04

euxassay_005538_05	euxassay_005538_06	euxassay_005538_07	euxassay_005538_08	euxassay_005538_09

euxassay_005538_10	euxassay_005538_11	euxassay_005538_12	euxassay_005538_13	euxassay_005538_14

euxassay_005538_15	euxassay_005538_16	euxassay_005538_17	euxassay_005538_18	euxassay_005538_19

euxassay_005538_20	euxassay_005538_21	euxassay_005538_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14638_wholemount_strong.wlz

14638_wholemount_moderate.wlz

14638_wholemount_weak.wlz

14638_wholemount_possible.wlz

14638_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14638_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8756
Entity Detected:	Dync1li2, dynein, cytoplasmic 1 light intermediate chain 2 ( MGI:107738)
Sequence:	sense strand is shown >T8756 CCGGCTGGCTGCCTGACCCAGCAACGTCCCTAGCTGCGGCGATCCCACGTCCCGCAGACGCACGAGGACA CCGCGGGCCCGTCGGGACGCGCTGGATAACGGAGCTTCTGCGGCGTTGCGGGCGGGCAGCGCGGCCCGGC CGGGGTCCTTGCGGGTCCTCGGCGGGCCCGGGGGAGGGCCCTCTTTGTGGCTGCAGTTGGCAAGATGGCG CCGGTGGGGGTGGAGAAGAAGCTGCTGCTGGGTCCCAACGGGCCCGCAGTGGCGGCCGCCGGCGACCTGA CAAGTGAAGAGGAGGAAGGCCAGAGTCTATGGTCTTCGATCTTGAGCGAAGTGTCCACCCGTGCCAGGTC CAAGCTGCCGTCCGGCAAAAACATTCTGGTGTTCGGTGAAGATGGTTCTGGTAAGACAACTCTCATGACC AAACTCCAAGGAGCAGAACACGGCAAAAAAGGAAGAGGCTTAGAGTATCTTTACCTCAGCGTTCATGATG AGGACCGGGACGATCACACACGCTGCAATGTGTGGATTTTAGATGGAGACCTCTACCATAAAGGCCTGCT GAAATTTGCAGTTTCAGCTGAGTCTCTGCGGGAGACCCTGGTCATTTTTGTTGCCGACATGTCTCGACCT TGGACGATAATGGAGTCTCTGCAGAAGTGGGCTAGTGTTTTACGGGAGCACATTGACAAGATGAAGATTC CACCGGAGGAGATGCGGGATCTGGAACGGAAATTTATGAAAGAGTTTCAAGATTATATCGAGCCTGAAGA AGGTTGTCAGGGTTCCCCACAGAGAAGAGGGCCTCTGACCTCAGGCTCAGACGAAGACAGTGTTGCCCTC CCTCTGGGTGACAACGTGCTGACTCATAACCTGGGGATCCCAGTGTTGGTGGTGTGCACAAAGTGTGATG CCATGAGCGTCTTGGAGAAGGAGCACGATTACAGGGACGAGCACCTGGACTTCATCCAGGCGCACCTGCG GAGATTCTGCCTCCAGTATGGAGCAGCCTTGATTTACACCTCAGTGAAGGAAGAGAAAAACCTTGACTTG CTGTATAAGTACATTGTTCATAAAACCTATGGCTTCCACTTTACCATACCCGCCTTAGTTGTGGAAAAGG ACGCGGTTTTTATACCTGCAGGCTGGGACAATGAAAAGAAAATAGCTATTTTACATGAAAATTTTACAAC CGTGAAGCCAGAAGACGCGTATGAAGATTTTATTGTAAAGCCGCCTGTGAGAAAGCTGGTCCATGACAAA AAGTTGGCAGCGGAGGATGAGCAGGTGTTCCTAATGAAGCAACAGTCACTCCTTGCCAAGCAGCCTGCCA CGCCCACAAGAACCTCCGAATCCCCTGCCAGAGGACCCTCTGGCTCTCCAAGGACGCAGGGTCGGGGTGG ACCAGCTAGTGTACCAAGTGCCTCCCCCGGAACTTCAGTAAAGAAGCCAGATCCAAACATCAAAAATAAT GCGGCAAGTGAAGGGGTATTGGCCAGCTTCTTCAATAGTCTGTTGAGTAAAAAAACAGGCTCTCCCGGAA GTCCTAGTGCTGGTGGAGTGCAGAGCACAGCCAAGAAGTCAGGACAAAAGACTGTGTTGTCAAATGTTCA GGAAGAACTGGATAGAATGACTCGAAAGCCAGACTCCATGGTAACAAACTCTTCAACAGAAAATGAAGCC TGACCCTCCTTTGAAAGTGCATCTGTCAAATGACCAAATAACTATGTATATTCATCTGATAAGACCAGGA TTTTTCTGATTCGGCACATGCTGTCAGTTTTTGGGGGTAGGGGAGATGAACTTTAAAACAATACATATAC TTCTTTGGCTTGTCCGAGTGTGAGATGCACATTTAGGAGGCTCCATGTCAGGCCCCTTCTTAGGGATGTC CCTTGGACTCTTGGGCTGCGATTCTCTTGTGGGAAGCAAGTGCAGCTGGCCTTGTAGAGTGGAGAAAGGA GGCCCACTTTCTATCAGGGTGGAGCAAAGGAGACTAGGAAGGCATTGTTCAGATGGATGGTTAGTTTGAC CAGTTGCGTGTTAGCAAATTCACTTGTTCTTGGGAAGCAGGTTAAGTAGCTGACAGTCCAGGAAAGAGAA AGGTTCCGGAGGGACGAGAGAAGTGCTGGTGGGGAAGCCGAAGGTTGAGGGAGGTGGAGGAAGGAAGCGA GTGAGCGGGCAGGCAGTGTTAGTAAGTACAAGCTCGGTAGAAACCAGATAGCAACAGTTAAGACCTAACA ATTAGAAACATTTCCAAACTAAGCACTAAACAGAATGGGAGCTGGGCTTGTTCTAAATGATCTTTAAGTT ATGGGAAATGTGTTTTATGTAGGGTGCTGAGTAAGGTGGGCTGACTCAGGGCTGGTGAAGACCACAGCTG CCCCCGGGACCCACATGCTTGACCCAGGCCATGTCCCTTGTCCTTGGAGAGATGTCGGGCTCTATGCAGA TGTAACCTGGGGCAGGCAGTCGCTGTGCGTTATGTTCAAAGTCAGCAGATACAAAACTAGATATTTTAAA TAGTTCCTGTTTTGTGTTGTTTAATTATTTTTTTAACTACCACAGGCTGGCCAACTAATAGAAAAAGGGT GATTGATTTCTCATTTTGTACCATGTTGGCAATGTTCTAGGGAAAGTGTGGGGGAAGTATTTAATGTCGT TACACTCTGGTTATTTGGCTTGTGCCAGGTCCTGCCTTAGAGCCTGTGGTAACCCTCCCTGATACAGAAA GGTTCTTTCTGTAGCTCCCTGGTCGGATCATTGCTGTCAGACCGACAGGTGGGTGCTTTCCATGGAGAGT GGAAGTGGTAGGCTTGTGCTTTCCATGAAACGTAGCACTTCTTCTCTTAAGGAGGTGCAAGTCTTCTGTG TCTTCCATCAGGGGCATGTTCATGAACACTGTCATGTCACACCACCATGCTGAAGATAACTTCTGTGGAT TTGAAATGGCACTTTGAGTTTCTGTGTGACACAGCACATAAATGCAAGCACTACCATGACTTTAATGTAT AAAATCTTTGTTATCCAAACCAAGGGGAAAACCACAGTTCACTTCCGTGGAGTTTACTCTTTTTTTATAT GAATGTTTGTTACTGTGTCTCGGCATAGTGTTCCTAATGTTGTTACATACATTCATATAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6405596 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6405596 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14641 same embryo
	EMAGE:14637 same embryo
	EMAGE:14640 same embryo
	EMAGE:14639 same embryo
	EMAGE:14636 same embryo
	EurExpress:euxassay_005538 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source