EMAGE mouse gene expression spatial database

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EMAGE:14648

Ryk receptor-like tyrosine kinase ( MGI:101766)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005574. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005574_01	euxassay_005574_02	euxassay_005574_03	euxassay_005574_04

euxassay_005574_05	euxassay_005574_06	euxassay_005574_07	euxassay_005574_08	euxassay_005574_09

euxassay_005574_10	euxassay_005574_11	euxassay_005574_12	euxassay_005574_13	euxassay_005574_14

euxassay_005574_15	euxassay_005574_16	euxassay_005574_17	euxassay_005574_18	euxassay_005574_19

euxassay_005574_20	euxassay_005574_21	euxassay_005574_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14648_wholemount_strong.wlz

14648_wholemount_moderate.wlz

14648_wholemount_weak.wlz

14648_wholemount_possible.wlz

14648_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14648_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5449
Entity Detected:	Ryk, receptor-like tyrosine kinase ( MGI:101766)
Sequence:	sense strand is shown >T5449 CCACGCGTCCGGTGAGCCTCTACCTGAGCGAGGACGAGGTGCGCCGGCTGCTTGGTCTTGATGCAGAGCT TTACTATGTGAGAAATGACCTCATCAGTCACTACGCTCTGTCCTTTAACCTGCTAGTGCCCAGTGAGACA AACTTCCTGCACTTCACTTGGCATGCAAAGTCCAAGGTTGAATATAAGCTGGGATTCCAAGTAGACAACT TTGTGGCTATGGGCATGCCCCAGGTCAATATTTCTGCTCAAGGGGAGGTCCCACGCACTTTATCAGTGTT TCGGGTCGAGCTTTCTTGTACCGGCAAAGTCGACTCTGAAGTCATGATTCTAATGCAGCTCAATCTGACA GTGAATTCCTCAAAAAATTTTACAGTTTTAAATTTTAAACGAAGGAAAATGTGCTACAAAAAACTTGAAG AAGTAAAAACTTCAGCCTTGGACAAAAACACTAGCAGAACTATTTATGACCCTGTCCATGCAGCGCCAAC GACTTCCACGCGTGTGTTTTACATCAGTGTAGGGGTTTGCTGTGCAGTGATATTTCTTGTAGCAATAATA TTAGCCGTTTTGCACCTTCATAGCATGAAAAGGATTGAACTGGATGACAGCATCAGCGCCAGCAGTAGTT CCCAGGGGCTGTCTCAGCCGTCTACCCAGACGACCCAGTATCTGAGAGCTGACACACCCAACAATGCAAC GCCTATCACCAGTTATCCTACCTTGCGGATAGAGAAGAACGACTTGCGAAGTGTCACTCTTCTGGAAGCC AAAGCCAAGGTGAAGGATATCGCAATATCCAGAGAAAGGATCACACTGAAAGATGTCCTCCAAGAAGGTA CTTTTGGGCGTATTTTCCATGGGATTTTAGTAGATGAAAAAGATCCAAATAAAGAGAAGCAAACATTTGT AAAAACAGTTAAAGACCAAGCATCTGAAGTTCAGGTGACGATGATGCTCACCGAGAGCTGCAAGCTTCGA GGTCTGCACCACAGAAACCTCCTTCCTATTACTCATGTGTGCATAGAAGAAGGAGAAAAGCCCATGGTGG TATTGCCATACATGAATTGGGGGAATCTTAAATTATTTCTTCGGCAGTGCAAATTAGTAGAAGCCAATAA TCCACAGGCAATTTCCCAGCAAGATCTGGTCCATATGGCTATTCAGATTGCCTGCGGGATGAGCTACCTG GCGAGGAGAGAAGTGATCCATAGAGACCTGGCTGCTAGGAACTGTGTCATCGACGACACTCTTCAAGTCA AGATCACAGACAATGCCCTTTCCAGAGACTTGTTTCCTATGGACTACCACTGCCTAGGGGACAACGAGAA CAGGCCAGTGAGATGGATGGCTCTGGAAAGTCTGGTTAATAATGAGTTCTCTAGTGCTAGTGACGTGTGG GCCTTTGGAGTGACGCTGTGGGAGCTCATGACTCTGGGCCAGACGCCCTACGTGGACATCGACCCCTTTG AGATGGCCGCTTACCTGAAAGATGGTTACCGAATAGCCCAGCCAATCAACTGCCCTGATGAACTGTTTGC TGTGATGGCCTGTTGCTGGGCCTTGGACCCTGAGGAGAGGCCTAAGTTCCAGCAGCTGGTCCAGTGCCTC ACAGAGTTCCACGCTGCCCTGGGAGCCTACGTCTGACTTCTCTCCCAGCTCCGCCACTCAGAAGAAAGTG CCTGTCTGTCACGGATGCCCCTCGTGCAGCGCAGTGCCTGCAGGGGCACACTGTCTCCAGATCACCCAGC CTTAGCAGTGCTTCCAAACCTCAGCTTTTAACGATGACGTAATAACGCAGAGTGTTTTCTAGATACCACT TTTACTAGGTTGAACCAAAAGGGGTTTTGTACATTTTTTGGCCAAAAATTTTTTTAAAATATAGTGACTT TGGACTAGGGGGTGCATTCTTTTAAAAAATGAATAAACAGTTTTAAAAATTAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5370100 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5370100 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14649 same embryo
	EMAGE:14653 same embryo
	EMAGE:14651 same embryo
	EMAGE:14650 same embryo
	EMAGE:14652 same embryo
	EurExpress:euxassay_005574 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source