EMAGE mouse gene expression spatial database

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EMAGE:14653

Mbd2 methyl-CpG binding domain protein 2 ( MGI:1333813)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005566. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005566_01	euxassay_005566_02	euxassay_005566_03	euxassay_005566_04

euxassay_005566_05	euxassay_005566_06	euxassay_005566_07	euxassay_005566_08	euxassay_005566_09

euxassay_005566_10	euxassay_005566_11	euxassay_005566_12	euxassay_005566_13	euxassay_005566_14

euxassay_005566_15	euxassay_005566_16	euxassay_005566_17	euxassay_005566_18	euxassay_005566_19

euxassay_005566_20	euxassay_005566_21	euxassay_005566_22	euxassay_005566_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14653_wholemount_strong.wlz

14653_wholemount_moderate.wlz

14653_wholemount_weak.wlz

14653_wholemount_possible.wlz

14653_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14653_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5520
Entity Detected:	Mbd2, methyl-CpG binding domain protein 2 ( MGI:1333813)
Sequence:	sense strand is shown >T5520 GTGCGCGCCCGCTCTATGATGCTTGCGCGCGTCCCCCGCGCGCCGCTCTGCGGGCGGGGCGGGTCTCCGG GATTCCAAGGGCTCGGTTACGGAAGAAGCGCAGAGCCGGCTGGGGAGGGGGCTGGATGCGCGCGCACCCG GGGGGAGGCCGCTGCTGCCCGGAGCAGGAGGAGGGGGAGAGCGCGGCGGGCGGCAGCGGCGCTGGCGGCG ACTCCGCCATAGAGCAGGGGGGCCAGGGCAGCGCGCTCGCTCCGTCCCCGGTGAGCGGCGTGCGCAGGGA AGGCGCTCGGGGCGGCGGCCGTGGCCGGGGGCGGTGGAAGCAGGCGGCCCGGGGCGGCGGCGTCTGTGGC CGTGGCCGTGGCCGTGGCCGGGGTCGGGGCCGTGGCCGGGGCCGGGGCCGGGGCCGCGGCCGTCCCCAGA GTGGCGGCAGCGGCCTTGGCGGCGACGGCGGCGGCGGCGCGGGCGGCTGCGGCGTCGGCAGCGGTGGCGG CGTCGCCCCCCGGCGGGATCCTGTCCCTTTCCCGTCGGGGAGCTCGGGGCCGGGGCCCAGGGGACCCCGG GCCACGGAGAGCGGGAAGAGGATGGACTGCCCGGCCCTCCCCCCCGGATGGAAGAAGGAGGAAGTGATCC GAAAATCAGGGCTCAGTGCTGGCAAGAGCGATGTCTACTACTTCAGTCCAAGTGGTAAGAAGTTCAGAAG TAAACCTCAGCTGGCAAGATACCTGGGAAATGCTGTTGACCTTAGCAGTTTTGACTTCAGGACCGGCAAG ATGATGCCTAGTAAATTACAGAAGAACAAGCAGAGACTCCGGAATGACCCCCTCAATCAGAACAAGGGTA AACCAGACCTGAACACAACATTGCCAATTAGACAAACTGCATCAATTTTCAAGCAACCAGTAACCAAATT CACGAACCACCCGAGCAATAAGGTGAAGTCAGACCCCCAGCGGATGAATGAACAACCACGTCAGCTTTTC TGGGAGAAGAGGCTACAAGGACTTAGCGCATCAGATGTAACAGAACAAATTATAAAAACCATGGAGCTAC CTAAAGGTCTTCAAGGAGTCGGTCCAGGTAGCAATGACGAGACCCTTCTGTCTGCTGTGGCCAGTGCTTT ACACACAAGCTCTGCGCCCATCACAGGACAAGTCTCTGCTGCCGTGGAAAAGAACCCTGCTGTTTGGCTT AACACATCTCAACCCCTCTGCAAAGCTTTCATTGTTACAGATGAAGACATTAGGAAACAGGAAGAGCGAG TCCAACAAGTACGCAAGAAACTGGAGGAGGCACTGATGGCCGACATCCTGTCCCGGGCTGCGGACACGGA GGAAGTAGACATTGACATGGACAGTGGAGATGAGGCGTAAGAATATGATCAGGTAACTTTCGACTGACCT TCCCCAAGAGCAAATTGCTAGAAACAGAATTAAAACATTTCCACTGGGTTTCGCCTGTAAGAAAAAGTGT ACCTGAGCACATAGCTTTTTAATAGCACTAACCAATGCCTTTTTAGATGTATTTTTGATGTATATATCTA TTATTCCAAATGATGTTTATTTTGAATCCTAGGACTTAAAATGAGTCTTTTATAATAGCAAGCAGGGCCC TTCCGGTGCAGTGCAGCTTTGAGGCCAGGTGCAGTCTACTGGAAAGGTAGCACTTACGTGAAATATTTGT TTCCCCCACAGTTTTAATATAAACAGATCAGGAGTACCAAATAAGTTTCCCAATTAAAGATTATTATACT TCACTGTATATAAACAGATTTTTATACTTTATTGAAAGAAGATACCTGTACATTCTTCCATCATCACTGT AAAGACAAATAAATGACTATATTCACAGACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6399206 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6399206 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14649 same embryo
	EMAGE:14651 same embryo
	EMAGE:14650 same embryo
	EMAGE:14648 same embryo
	EMAGE:14652 same embryo
	EurExpress:euxassay_005566 same experiment
	MGI:4826115 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source