EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14654

Zfp449 zinc finger protein 449 ( MGI:1925869)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005532. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005532_01	euxassay_005532_02	euxassay_005532_03	euxassay_005532_04

euxassay_005532_05	euxassay_005532_06	euxassay_005532_07	euxassay_005532_08	euxassay_005532_09

euxassay_005532_10	euxassay_005532_11	euxassay_005532_12	euxassay_005532_13	euxassay_005532_14

euxassay_005532_15	euxassay_005532_16	euxassay_005532_17	euxassay_005532_18	euxassay_005532_19

euxassay_005532_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14654_wholemount_strong.wlz

14654_wholemount_moderate.wlz

14654_wholemount_weak.wlz

14654_wholemount_possible.wlz

14654_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14654_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9602
Entity Detected:	Zfp449, zinc finger protein 449 ( MGI:1925869)
Sequence:	sense strand is shown >T9602 CTGTAAACTGCTAGAAGAGGTGATGGCTGTGGCCCTGGGCTGTGCAATCCAGGCCTCCTTGAATCAGGGC TCTGTGCTGCAAGAGTATGACACAGACTGTGAAGTTTTCCGACAGCGTTTCAGGCAGTTCCAGTACACAG AAGCTGCCGGGCCTCATGAAGCATTCAACAAACTCTGGGAGCTTTGCTGTCAGTGGCTGAAGCCAAAGAT GCGTTCTAAGGAACAAATCCTGGAACTGCTTGTGCTAGAGCAATTCCTAACTATTCTGCCCACAGAAATA GAGACCTGGGTGAGGGAACACTGCCCAGACAATAGAGAAAGAGTTGTGTCACTCATTGAAGACTTACAAA GAGAGCTTGAGATACCAGAACCACAGATTGACATGGATGACATGCTCTTGGAAGAACTGGCGCCAGTTGG AACAGTACCCATGCCACCCAACTTGCACCTGGAGGAGCCTTCACTCCAGGTGATGGAACCTGTTCAGGAG CCTCCGGTGCCGGATGCCTGGATTCCACAAGCTGGCCCCCAGGATCTGAACTACAGTGCTGATGCTGAAT GCCAGTCCTTTCTGGACCCTGGTTATCAGTTACCAAAGCTTGACATGAACTTCCCATTGGATCATAGAGA AGAACCATGGGTAAAAGACTTAGAGGATCCCAAAGAAATGAAACAATTACTTGATTCCAAGATTGGTTTT GAGATGGGAATAGAAAATGAAGAAGATACTTCAAAGGAAAAAAAACTGGAAACTTTGTACCCATTTGTTG TAACTTTAGAAGGGAATGCTCTCCATGGTCCCATTTTGCAGAAAGACTATGTGCAGTTAGAAAACCAATG GGAAACATCCCCAGAGGACTTACAGACAGACCTAACAAAACTCGTAGATCCTCAGAACCCAACTCTAGGA GAGACACCCGAGAGCCCCAGCCTGGAAGAACCTCTCACCCCGAAGCCTCCAAAGAAAAAGAGTCCTGGGG ACAAACCTCACCGATGTCCCCAGTGTGGCAAATGTTTCGCTCGGAAGTCACAACTTACAGGGCACCAGCG AATTCATTCAGGAGAAGAACCTCACAAGTGCCCTGAATGCGGAAAAAGGTTCCTGCGTAGCTCTGACCTT TACAGACACCAACGACTTCACACAGGAGAAAGACCCTATGAATGCACTGTATGTAAAAAGCGATTCACTA GGCGCTCACACCTTCTTGGGCACCAGAGAACCCATTCTGAAGAAGAAACATATAAATGTCTTGAGTGTGG AAAAACCTTTTGTCATGGGTCAAGTCTTAAAAGACATCTAAAAACTCATACAGGTGAAAAGCCTCATAGA TGTCCTAATTGTGGGAAAAGTTTTAGTCGCCTGACGGCACTTACTTTGCACCAGAGAACACACACGGAAG AGAGACCTTTTAAATGTAGTTACTGTGGGAAAAGCTTTAGACAGAGACCAAGCCTTGTCATTCATTTAAG AATCCATACAGGGGAAAAGCCATACAAGTGTACTCATTGTTCTAAAAGCTTCCGGCAGAGAGCAGGCCTT ATTATGCACCAGGTCACACACTTTAGAGGACTTCTTTAAGAACCGTGAAGGGAAACAGGTTCTATCAACG TTAACACTAGCTCAGAAACAGTATAGGCTGCTGTAGATGTTTGTCTCATAAGCCTTTTGTCTGAACTTGT AAGTGCAGCTTTGAGGGGTGCTATTTTTAAAAATACATCTTTCAAGGTATATGAGTTCTAGAGTGTCCAC ATGTTTGTGCAGTTTCTCTGAATTTAATATCTTCTGTCTTTTTCTGTTGTTTAATTGCAGTGGAAAGGTT TGGTTCCAAGGCAACCATATGATAAGCATAAGCACAAAGCATATAAACGGTGGCAGTCGTTTATAGGTAG GACCAGTTAATGGATGAGTCCTGGTAACGGTTACAGTCCTACCACACTTGTTTTCCTGTGTCATTATGAA AGCAGCCAGTAGATAGATGTGCCCCAAGTGGCAAGAAATGAGACAGTGAATTTTGTCAACACATTAAAAT TTTGTTTCAGGGGGACTAGGGTGAAGAGGATGTCATTGGCCTAATGAACAGGCTTGTTTCCAATTACAGT TTTATGCTTAGAGAAGTTGGGATCTTAAGTTTGGGATTTTGTGGAGTTTGCTGGTCTGCTTAGTTGGCTA TCTGTGGTGATGGTGATTAGAGTGTGGTGACTAGAGTGGGGTGTTCTCTTTATAACTGTATGCTAGGTCT AGCAGAAGTAACTTACAGTTGATGTTGTACAAGTAGACTCTGACTGTATTTAGATGGACTGAAGAACAAG TCTGCATGGATTTTCATCCTGCTTCAGGATTCTCTCTGGTTTATTTCAATGCTTTTGAAATTTTATCCGT TCTCCAAGTTGGTGTTTTTCTAAGTATGTGTCATAGAATACTTTGTTCTGCAAGATGTTCTACAAATAAT TTTGGGGATTGCTTCTAGCTGTTTGTCCCTCCTAGACTCATGATACTCATCAGCATATGAGCCCCTGGGA AGGTCTTAGCTTAGAAAGGAGGCTCCTCTTGTAGCTCAATGCGTTCCAAACTTATTTGACCCTAGAACAT TTTTCATGTACACATACTAGTAACTTGCAGTAGTATTCTGCTAGTATCCCTTGGGACACACTGCCTTAAA CCAGTATGTCTATAGCTATCAGTGTAGTCCTGTCACTCTAAACCTAGTTATTTCTAGTTTCAATGATTTT ATGAAAAAATTTGAAAATACACTTATTTTGTATTATATACACATCATTTCAAGGACGTTAAAAATCACTA AGTCACAAAATTTGTGAGTTACTTAGTGATTTGTAGCTTCTGTGTTTTAATTATACTTAGACACACACAA CATTTTAAGAGGTGAAGCTCACTAATTCACATAATTTATGAATTATACAGTGATTCTTAACCTTTTCGCA TTTTACTTTATAATCTCACACACAAGCACACACACACACACACACACACACACACACAGAGTTGGTACTT ATATAAGTGTTCTCATGTAATCATAGTAACAACTCAGATCTCACAGTAAGGCAGAAAATCCTCATTGAGC TTAGTCCTTGTTATTAGATACAAGAAATGGGACAGTCAAGTATATTCCCTTTCTGAAGCTACACAGTCAT CTTTAAAGTAGAACTAAGGGAGTTCTTGGGAGTAATCTATAAGTATTTGGCGGGTATGTATTTTGTGCCT AATTTGTCCTGTCCTAGTCTTAATGGAAGATAGTAACAAAACATAATTCATATGTAAGGCTCATAAAGAG TTTATCATATCTCTACTTAAACAAGAGTTGGGAAACAAATGGAGAACAATGCAAGAGAGAGCTTGTGACT AAGTGCCAAATCATATAAAGTGCCAGAGCCAGGATGTGTCTTTTTTCTTGGCATCCAGGTCCCTGTATGA TGCTGCTAATAATTCTGGGATTAAAATTACACCCCAGTAACATGGGAGGAGTCTGAATGTAGAGATTAAA AATACAGAATGTAATGGCTTCTCTTTCTCTCTTGTGGAATTGCATTCAAACCAGAACAGTGCCTTAGAAT GGATGTGCCCAACTGCTCTGTATTTATACACGGTTTTAATAAAGTGGAAATGTATATAAAAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30642886 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30642886 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14656 same embryo
	EMAGE:14658 same embryo
	EMAGE:14655 same embryo
	EMAGE:14657 same embryo
	EMAGE:14659 same embryo
	EurExpress:euxassay_005532 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source