EMAGE mouse gene expression spatial database

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EMAGE:14656

Zfp760 zinc finger protein 760 ( MGI:2679257)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005519. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005519_01	euxassay_005519_02	euxassay_005519_03	euxassay_005519_04

euxassay_005519_05	euxassay_005519_06	euxassay_005519_07	euxassay_005519_08	euxassay_005519_09

euxassay_005519_10	euxassay_005519_11	euxassay_005519_12	euxassay_005519_13	euxassay_005519_14

euxassay_005519_15	euxassay_005519_16	euxassay_005519_17	euxassay_005519_18	euxassay_005519_19

euxassay_005519_20	euxassay_005519_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14656_wholemount_strong.wlz

14656_wholemount_moderate.wlz

14656_wholemount_weak.wlz

14656_wholemount_possible.wlz

14656_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14656_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9594
Entity Detected:	Zfp760, zinc finger protein 760 ( MGI:2679257)
Sequence:	sense strand is shown >T9594 ACTGGCCAGAGTTTCAAGTAGATGTGAAAACTTCCTGCAGCCACTGGAGCCTCGTAGAACGGTCTGGGAG ACTCGCCGGTCCGCACCACAGTGGCGGGAACCGCAGCCCGGCGATCCTAGCATCTCCCAGGTCAGAAGAG CTGTAGTAAACAGGGTTGTTGACATGCGGTGAACTTCTCTCAGGAAGAAGAGGAAATGCCTGGACCTCAC TCATAGGGTTTTGTACTGAGATTGAATATTGCAGAATTGCAGCAGTCCTGTTTCTGGCTTGCACTTTTCA GCTACACCTGCTTATCGTCGAGCAGGACAAAGTGCCCTGGAAGGTGGAGAGAGAGGCATCAGTAGCAAAG GATCCAGGCAAGAAGGGGTCCAGCCATTGTTCTTGAGACAGAAACTCAGAGCAGATCAACAGGAAGAAAT GGCTGCTTCTCCAGGTCTGTTGACATTCGGGGATGTGGCTTTGGACTTCTCCCAGGAGGAGTGGGAATGC CTGGACTCTGCTCAGAGGGCTCTGTACATTGATGTGATGTTGGAAAATTACATCAACTTGGTCTCTGTTG AGAAGCATCACATGTGTGGTAAATATGAGAAGGTCTTGGATCAACACTCAGAGCATCAACATGTGAATAT CCAAGAAAAGTCTTACAAATATAAAGAGCTTGGCAAAATGGTTCATGAATCCTCTCCATGTACACCTTAT AACACAAGTAGTACTGCAGAAAACTGTAACAAGTACAGCTACCAGGATGCTTCCATTGAATCATCCCTAA TCAGACATAATAGTGTACACACTGGAGAAGAACTTTTCAAATATAAAGAGTGTGGCAAATGTTTATGTTC CAACATTTGTCCAAATCAGAGTATCCACGATATAAAGATGGAACACAAGTATACAGAAAGTGATAAGCTT TGTGGCTCTACACACAGACTTGTACAGCAAAGAACTCATAGTGGAGAGAAAGCACACAATTGTGGGAAAT GCTTTAGGAGTTGCTCAAGCCTCAGTACACATCAGAGACTTCACACTGGAGAGAAACCATGGAAATGTAA AGAATGTGGTAAGTCCTTTAAGTGGTTGTCTCATCTTAAAGCCCATTGCAGAATCCATACTGGAGAAAAA CCTTTCAAATGTAATGAATGTGATAAAGGCTTTTCACGGTTATCTAGACTTAAAAGTCATTATAGAACCC ATACTGGAGAGAAGCCTTACAAATGTGAGGAATGCGGTAAGTGCTTTTCTCGGTTGTCAAGACTTAAAAA CCATTATAGAATCCATACAGGAGAGAAACCTTACAAGTGTAAGGAATGTGGCAAATCTTTTACCCATTGC ACGGGTCTTAGTACACATCAGAAAATTCATACTGGGGAGAAACCTCACAAATGCAAAAACTGTGGTAAAT CCTTTCATCTGCTGTCAACTCTTAAAGAACATTTCAGAGTCCACACTAGAGAGAAGCCTTACAAGTGCAA AGAATGTGGTAAGTTCTTCCATTGGTTGTCAGGCCTTAAAAGCCACTACAGAATCCACACTGGAGAGACA CCTTACAAATGTAAGGAGTGTGACAAGTCATTTTCTCAGTTATCAAGACTTAAAAGCCATTATAGAACCC ATACTGGAGAGAAATCTTGCAAATGTTTAGAATGTGACAAATACTTTACCCAATGCACAGCTCTTAAAAG ACATCAGAAAATTCATACTGATGAAAAACCCTACAAATGTGAAGAGTGCAGCAAATGTTTTTATGTGTTG TCTCTCCTAAAAAGCCATTGCAGAACCCATACTGGAGAGAAGCCTTACAAATGTGAGGAATGTGGTAAGT GCCTTTCTCAGTTGTCAAAACTTAAAAGCCATTATAGAATCCATACAGGAGAGAAACCTTATAAGTGTAA GGAATGCGACAAATCCTTTACCCATTGCACAGCTCTTAGTACACATCAGAAAATTCATACTGGAGAGAAA CCCTACTCATGCAAAGACTGTGGTAAATCCTTTTATCTGTTATCAAGACTTAAAGAACATTACAGAGTCC ACACTGGAGAGAAACCGTACAATTGTAAGGAATGTGGTAAGTTCTTCCATTGGTTGTCAAACCTTAAAAG ACATTCCAGAATTCACACTGGAGAGAAACCTTACAAATGTGAGGAATGTGACAAATCCTTTACCCACTGC TCAAGTCTTAAAGCTCATCAGAAAATTCATGCTGGGGTAAAACCCTACAAATGTGAAGAATGTAGCAAGT CCTTTTATTGGTTGTCAAACCTTAAAACCCATTCCAGAATCCACACTGGAGAAAAACCTTACAAATGTGG TAAATGTGACAAATCCTTTACCCAGCGCTCTCTTCTTACAAAGCACAACAGGATCCATACTAGAGAAAAA CATGAAAACAGGAAATATGTGAGAACTTTTGATGAATGATTAAATCATAGAAAATTCCAAGAGTTTATAA CTAATTCATGAGGACAAAATGTAGTCAGAAATTCAAACCAGAACTAAACCAGAAGAATGTTCACTGTCTT AAAATCCTCATATCTTATGCATTAGAAATTCCATGCTAGAAAACCCATGATATGCATTGATTTTTTTTTT TCAGTTTTTACCTAGTGCTAAAATTGTAGATACATCTGAAAATTCATCCTGAAGGGAAACGCAAGAATCT CCTTTAATAATGAGTCCTCTTTTTTTTTTTTTTTTTAACCTTCAAAATAATCATTCAGGAGTCAAATTGT ACAGACAGCGTGAGTATGAGAAATGTTAAAGAGATAACTTGTGGATGTGATACATGTACATGCATAGCCT GGAAGTCCCAAAGAATGTGCAGGAAAATTGGTTTTCCTGTACTCAAGAAAATTGGAAAAGTATTTAGTCC AACTGAAAAACACAATGATTCATATAGAAACACTAAGACACAGACATTAGATAAACAAGTGTATTGCAAA GAAGGAAACCAAATGCGTAAAGGAATTTGTTATGTACTTGAGTGGAGGAGGATATTTCTATTTGAGTAAA TATATAATTCATATTAACATTTGGGTAATGCTAGTATTGGAATTTGATGAAATGTAAACACAATAGTTTT TTTTTTTTATACTCAGATGCATCTATGCTGTCTTGCTTACAGATGATTTTATGATAATGTGTATCTATTG TTGCTGCTTCAGTGCTGATGAATCTGAGTCAGGAGTGGTGAAGTTAGGTAGCTAATGACTGCTAACCATC TGGTGAGTCCAACTATCTAGACCGACTCATGATGGCAAATTTAGAGTCTCCCTGGGTCACGTCTTCATGC AGATGCTGCACAGACCAAGGCCTCTCTGCTTGATGACCTTTTGAAAACTATACTAGTTCATGGCAGTGTT ACAGTCCCAGAGAGAATTCTGTCATTGTGTGCCTGTGAAGACCACTTTAAAAAAAAAACAAAAAACAAAA AACAAACAAACACTTATCTCCGATTATTTTCTTCATTGGTTCACTATGTAAAAAGCCATGGTAACCTGAG CAGAATCCTGTCTTGGAATCCCTTTGGTTTCAGGAGTTATTCTTCGCCTTTATTTGTTGTTACATGGTGG TTGAAGTGCCCTACTCTGTTGGAGTTCACTTTTCTTTGTGTGAGCAGGATTCTGAGATCATGGTACCTTA CAGGCAATGTAGCTTGCTGCCTGTTGCTCCTAACAATCTACTCTCTGTTAATAACGTGTCGTTAATATCA CTTGGTCCATGAAAAATTAGTAGTCTAAGCAAGCTTCAATACTGTAACACAAATGGAGATGAGTTTTATT TTGCAGGAGGAACACGTGTATGATGTATGAGCAATAGGAAGGGAAAAGGGCTATCCAAGTCTTCAATAGG CAGACACAGGGGGATCTCAATAATATATTTTAGAAATATCTGAAAAATGATAAGTCCATAGAATTGCTGA TCTCTATCTCGATATCCTCGATGAAATTTATGTAAAAAAATAGTTATCAAAGGACAGTCAAATCTAATAT TTGAGAATATATAGTTGTATTGAACAATGTGTACCATGAAACTCACACCTTGGCTTTCTTTGAAAATGGT CAATAGTAACTGTAGGTTTCCATATTTTAGTAAAGTAACATCCCTCTTATTGTTATCTATACTTTTTGTA AAACCACATTTATTTTTAAATGTTCTACAACTCTATGTAAGGTGATTTTTTTAAATAAATAAAACATCTG TGTTTAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30653533 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30653533 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14658 same embryo
	EMAGE:14655 same embryo
	EMAGE:14657 same embryo
	EMAGE:14654 same embryo
	EMAGE:14659 same embryo
	EurExpress:euxassay_005519 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source