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EMAGE:14661

Usp39 ubiquitin specific peptidase 39 ( MGI:107622)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14661 EMAGE:14661 EMAGE:14661 EMAGE:14661 EMAGE:14661
"Pseudo-wholemount" of euxassay_005529. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005529_01 euxassay_005529_02 euxassay_005529_03 euxassay_005529_04
EMAGE:14661 EMAGE:14661 EMAGE:14661 EMAGE:14661 EMAGE:14661
euxassay_005529_05 euxassay_005529_06 euxassay_005529_07 euxassay_005529_08 euxassay_005529_09
EMAGE:14661 EMAGE:14661 EMAGE:14661 EMAGE:14661 EMAGE:14661
euxassay_005529_10 euxassay_005529_11 euxassay_005529_12 euxassay_005529_13 euxassay_005529_14
EMAGE:14661 EMAGE:14661 EMAGE:14661 EMAGE:14661 EMAGE:14661
euxassay_005529_15 euxassay_005529_16 euxassay_005529_17 euxassay_005529_18 euxassay_005529_19
EMAGE:14661 EMAGE:14661 EMAGE:14661 EMAGE:14661 EMAGE:14661
euxassay_005529_20 euxassay_005529_21 euxassay_005529_22 euxassay_005529_23 euxassay_005529_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14661Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14661_wholemount_strong.wlz
14661_wholemount_moderate.wlz
14661_wholemount_weak.wlz
14661_wholemount_possible.wlz
14661_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14661_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5400
Entity Detected:Usp39, ubiquitin specific peptidase 39 ( MGI:107622)
Sequence:sense strand is shown

>T5400
GATGTCTAGCCGGTCCAAGCGGCAGTCCCATGGTTCCACCCGCGGGAAGCGTGAGTCCGAGTCTCGTGGC
AGCTCGGGTCGCATCAAGAAGGAGCGAGACCGTGAGAAGGAGCCCGAGGCGGCGAGCTCCCGGGGTAGCC
CGGTCCGCGTGAAGCGGGAGGCCGAGCCGGCTGCGCGAGAGGTCCCGGCGCCCGCGCTCCCGGTCGTGCG
GGTGAAGCGTGAGCGCGAGGCCGATGAAGACTCGGAGCCCGAGCGGGAGGTGCGAGCAAAGAATGGCCGA
GTGGATTCTGAAGACCGGAGGAGTCGTCACTGCCCGTACTTGGATACCATTAATAGGAGTGTGCTGGACT
TCGACTTTGAGAAACTCTGCTCCATTTCTCTCTCGCACATCAACGCATACGCCTGTCTGGTGTGTGGCAA
GTACTTTCAAGGCCGGGGCTTAAAGTCTCATGCCTACATCCACAGTGTCCAGTTCAGCCACCATGTCTTC
CTCAACCTCCACACTCTCAAGTTTTACTGCCTTCCTGACAACTATGAAATCATTGATTCCTCGCTGGAGG
ATATCACGTATGTGTTGAAGCCTACTTTCACAAAGCAGCAAATTGCAAACTTGGATAAGCAAGCCAAATT
GTCCCGGGCATATGATGGCACCACTTACCTGCCAGGGATCGTGGGACTGAACAACATAAAAGCCAACGAC
TATGCAAATGCTGTGCTGCAGGCTCTATCTAATGTCCCTCCTCTTCGGAACTACTTCCTGGAGGAAGACA
ATTACAAGAACATCAAGCGTCCTCCGGGGGACATCATGTTCTTGTTGGTTCAGCGTTTTGGAGAGCTGAT
GAGAAAGCTCTGGAACCCCCGAAACTTCAAGGCGCATGTCTCTCCTCATGAGATGCTTCAGGCTGTCGTA
CTTTGCAGCAAGAAGACTTTCCAAATTACCAAACAAGGGGATGGAGTTGACTTCCTGTCTTGGTTTCTGA
ATGCTCTGCACTCTGCTCTGGGAGGCACCAAGAAGAAGAAAAAGACTATTGTGAATGATGTTTTCCAGGG
ATCAATGAGAATTTTCACCAAAAAGCTTCCTCATCCTGATCTGCCAGCGGAAGAGAAAGAGCAGCTGCTC
CACAATGATGAGTACCAAGAGACGATGGTAGAGTCCACGTTCATGTACCTGACGCTGGACCTTCCCACGG
CCCCGCTCTATAAGGATGAGAAGGAGCAGCTCATCATCCCCCAGGTGCCTCTCTTCAACATCCTGGCCAA
GTTCAACGGCATCACGGAGAAGGAATACAAGACTTATAAGGAGAACTTCCTGAAACGCTTCCAGCTCACC
AAGCTGCCTCCGTATCTAATCTTTTGCATCAAGAGATTTACTAAGAACAACTTCTTTGTGGAGAAGAATC
CAACAATTGTCAACTTCCCTATCACAAATGTGGATCTGAGAGAATACTTATCTGAAGAAGTCCAAGCCGT
CCACAAGAACACCACCTATGATCTCATCGCCAACATCGTGCATGATGGCAAGCCCTCTGAGGGCTCCTAC
AGGATCCACGTGCTTCATCATGGGACTGGCAAGTGGTATGAATTACAAGACCTCCAGGTGACAGACATTC
TGCCCCAGATGATCACACTGTCTGAGGCGTACATTCAGATTTGGAAGAGGCGGGACAATGATGAAACCAA
CCAGCAGGGGGCTTGAACGCCTTGTTGGGTTTTTATTCCCAAGGGCTGTGGCTGAAGATGGTAAATAAAA
ATACTGAAGCTGTCGCTGAACACAGACTGACTGAAAGATTCCTGCCCCAGTCCAGTTTTTATGGATTCTG
GCTGCATCACCATGAAGGAGCCTGTCTGCCCTGATGTTCTGCGCTGTCTCCGGCAGCACGTTGTGGTCGA
CACTCTTCCCTTTCAGCATCAGCAGGCAGAACTCTTACAGATGGATGCCTTATTTGAATTGCTGCAAGTA
TCAAAGAACAGACAGTTCGGTAGAAACGTCAGGTCTGGAGACCCTAGGAGTAAACGTGAAGTCAGCAGCA
GCCTTCTGTCATAGGTAGTGGCTTTCCTGTAGCTCCCTTCATCTCCTGTGGGACGTGTGCGATGGATTAA
ATATCCTCCTGTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5365862 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5365862 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14663 same embryo
 EMAGE:14666 same embryo
 EMAGE:14662 same embryo
 EMAGE:14665 same embryo
 EMAGE:14664 same embryo
 EurExpress:euxassay_005529 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS