EMAGE mouse gene expression spatial database

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EMAGE:14665

Hmgcll1 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase-like 1 ( MGI:2446108)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005542. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005542_01	euxassay_005542_02	euxassay_005542_03	euxassay_005542_04

euxassay_005542_05	euxassay_005542_06	euxassay_005542_07	euxassay_005542_08	euxassay_005542_09

euxassay_005542_10	euxassay_005542_11	euxassay_005542_12	euxassay_005542_13	euxassay_005542_14

euxassay_005542_15	euxassay_005542_16	euxassay_005542_17	euxassay_005542_18	euxassay_005542_19

euxassay_005542_20	euxassay_005542_21	euxassay_005542_22	euxassay_005542_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14665_wholemount_strong.wlz

14665_wholemount_moderate.wlz

14665_wholemount_weak.wlz

14665_wholemount_possible.wlz

14665_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14665_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5427
Entity Detected:	Hmgcll1, 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase-like 1 ( MGI:2446108)
Sequence:	sense strand is shown >T5427 CGGACCCCGGGCACCCGCGGGACGCGCTCCCGGCTCTCACCCGGCTCTCGGCTCCGGCCGTGGGATCCAG CACCGCCCACGGCACCTAGGCTGCTGCTATGGGGAATCTACCATCTGCTGCGAAGCACTGCCTCAACTAC CAGCAGCTTCTCCGCGAACATCTCTGGAGCGGAGACTCAGTGGCAGGGGCGCTGGATGCGGCGCAGGAAG CATCTCAGTTACCTGGACTCCCTGAATATGTGAAAATAGTCGAAGTTGGACCAAGGGATGGATTGCAGAA TGAAAAGGTTATAGTTCCCACAGACATAAAAATTGAACTCATCAATCAACTTTCCCAAACTGGCTTGTCT GTAATAGAAGTAACCAGCTTTGTGTCTTCCAGATGGGTACCACAGATGGCTGACCATGCTGAAGTGATGC GAGGCATTCGTCAGTATCCAGGAGTTCGCTATCCTGTCCTTACACCTAATCTTCAGGGTTTTCAGCATGC TGTTGCTGCTGGAGCCACGGAGATAGCAGTTTTTGGTGCTGCTTCAGAATCTTTTAGCAAGAAGAATATT AACTGCTCCATTGAAGAAAGTATGGGAAGGTTTCAGGAGGTCATCAGCTCTGCAAGACACATGGACATCC CAGTGCGAGGGTACGTGTCCTGCGCCCTGGGCTGCCCATATGAAGGAAGCATCACACCACAGAAAGTCAC AGAAGTTTCAAAGAGATTGTATGGTATGGGCTGCTATGAAATATCTTTGGGCGACACGATTGGAGTTGGT ACTCCAGGCAGCATGAAGATGATGTTGGAAAGTGTCATGAAAGAAATCCCTCCAGGGGCTCTTGCTGTTC ATTGCCATGACACATATGGACAAGCCCTCGCAAATATCCTAACAGCTCTTCAGATGGGGATTAATGTGGT GGATTCTGCTGTATCCGGATTAGGTGGCTGCCCTTATGCAAAAGGTGCTTCTGGGAATGTAGCCACTGAG GATTTGATATATATGCTCAATGGCATGGGGCTCAATACAGGTGTGGATCTCTACAAGGTGATGGAAGCTG GTGAGTTTATTTGCAAGGCAGTGAACAAAACTACAAACTCTAAAGTCGCACAAGCCTCCTTCAACGCTCG CCTTGAATGAGTTTGTGATTTAAGAAAGAGCAGAGCCCTTCCAGCCCTAGTTCATCACAGTGTCATTAGT GCCAACACATTCTGAAGTGGAAGTTAATAAAAAGAGCTGGGAGAGGAGCTATGCTTGGATAGACCGTGAA GTGGATGGGGCAAGACAAGGCGTCTGTTCAACTGCAAGCTGAGAATGAGCCCCACTGCTTGCCCTTCTGG TGGTGGACAAAGCCTGCTCGTTTGCTCTTACAGAAATCATTATATTTTTTAGCTTAGTAGAACTGAAAAT TGACTTCAGATTTCTCTAAGTATCAAGTGCTGTGTTAATGCCTCCTCAAGCCACCTTACCGCAGGGTACA CATTGTTAGTGGTTTATGAGCCTCGACACAATGCAGAGTGTGTGGCCCTGAAGTTGTGTTTCCCCCCCCC CCTCTGGATCCTAACTCTCCATTTGCCAAGTTTGTTAAGTTGTGGACCAAGAGCCAAAGTTTCATCCGAC CCAACATATTTTTTAGCAATAGAACTTTTATATTTCGAGTGCGGCTAACATCTGTTAACTATTTCAATGA CTTTATTGTGCTCCAAGATGCTGTGGTCAATGGCAAGAAGCCACATCCTGAAAACACACCATACCTCCCA TGTTTGTTCTGTGCATCCAGTTTGCTGTGTTTTACATTTAATCAATTGTAGAAAAAAAATCCACAATGTT ATTTAAATATGGAGAAACTCTATGGTATGGTTTTTATTCTGGTGTCTAAGGGACAGTAACAATAGTAGAC TGATATAGACACCCTAGTCTATTTCCATAATATCTAGTTTACTTTTAAATGGCCCAAGGAAATACTACTG TGATTTTAAGAATGTTAATATAATTTTTAACTGATGAAAAGTCTGTTTTCATTGACTTATTATTTGTATT CTATATCTGTTTGTTTTTATTTGACTGTAGAATATTTATTTTAAAGTTGAATGACAGAAATATTATCTCC AGTGCCAGAAAGCATTGCCAGGGATAATGAAAATTAATACATTAAAGATCAAGGCCTGGGATATGGCTCA GTGGTTAAGAGCATTGACTGTTCTTTTAGAGGACATAGATTTGATTCCAAGCACCTGTATGGTGATTCAA TAGTATTTGTAAATCCAGTTTCAGGAAATCTAATGACTTCTTCTAGCCTCTGCATGACTAGTCAGGCAAG CAAGATACAGTCATATATGCAGGTAAATTACCAATTAACAAAAAACAGCACAGACATCCTAGAAAATAGT AAAACCATTGAAATGAAAATATTACAGAAAATAAAAGGGATATTGATTAGAAGCAAAATATCATTAAAGG TATATAGTAGGAAAAATTGGAACAGAAACATACGAGTTGACAAAGAAACCTAAATTTCCATGGAAGACTC CAACTTGTCAGCTTGTAACCTTTCTTCTGTAGGTTTTGTTCCAGCATCCTTTTCTTCTAGAGTACTGAAA GCAGTTCTGTGCTTGGGGGCTCAGGTTGGAATATGGATGACACAGAGGCAGCAAAGTGTCATCCAACTGT GGATCTGCCTTAGTCCTAGCTCCCTGCTCATCTCTCTTTCAGACTAGGGCACTGACAGGCGGCAGTGGTG GCGGCGGTGGCAAAAGTGCACTTCTTCCTTCATTTTCTGAGGAAGTTGATTTTTCCCTCTCAGAGACCTG GAGTGCTCAGTTTTGAGATATTTGTGAGAACATGTGTTTATGCAGACTACTTGTAAAAACAGTGACCAGC ATTTTTGTCTTGAAATGAAGCTCAAAGTTCTTTCAAAGACAGTGCAAATAGAAACCTCAGAAATAGTGTT ATACAGTTATATGGTGCCTACCTCAGTGTACCAGTTGACTTTCATGACCACCTTGATATTATCTAATTGC ACTTGACACAGACTAGTTTATATGTTCTTATCTCTCCCTCTTTCTCTCCCTATACTGTCTTAATTTCTTA GGAAGAGTATTCTCAACTTCTTTCAGCTATCCAGGAAACCTTGCAGTCATTGCATGACCCATTGGAAAAA ACAAACCAAAAAGCTCACCCACATGTATTTTGATACCAAAGTTTGCTTTGGTTTTACCTAGAAAGGCTTA AGAATGACAAGGCAAGTATGACTTTTGTTGAAATGTGAATGAAAATAATCTTTCTTCACATCTCTCCTTA AGGTAAGACTAGCTATTCTGTCTCCTGGAACTTGAAGTATGTTTTATCATAGAGATATGTTGCTGTTCTA TTATTGCTAGGACTGTTGGTAAGTAGCTGATCACTCTACCTAGGATGTGTAAGAACCTGTGTTTGATCCC TCAGCCCAACAAACCAACAAGCAGAAATAATTTTGCTTTTTGAAAATTAAGAATTTTTAAATCTGATGTT CATGAACTTAGAGCAAAAAAATTTAATCTGGTAACTTTCTAGTAAATTTGATGTTCTCTTTACATTTTTA GCATTTTGTTTACCATAAAATTTAATAAAGACTTTTTTAGCTGTAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5368383 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5368383 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14663 same embryo
	EMAGE:14661 same embryo
	EMAGE:14666 same embryo
	EMAGE:14662 same embryo
	EMAGE:14664 same embryo
	EurExpress:euxassay_005542 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source