EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14666

Wdr17 WD repeat domain 17 ( MGI:1924662)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005530. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005530_01	euxassay_005530_02	euxassay_005530_03	euxassay_005530_04

euxassay_005530_05	euxassay_005530_06	euxassay_005530_07	euxassay_005530_08	euxassay_005530_09

euxassay_005530_10	euxassay_005530_11	euxassay_005530_12	euxassay_005530_13	euxassay_005530_14

euxassay_005530_15	euxassay_005530_16	euxassay_005530_17	euxassay_005530_18	euxassay_005530_19

euxassay_005530_20	euxassay_005530_21	euxassay_005530_22	euxassay_005530_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14666_wholemount_strong.wlz

14666_wholemount_moderate.wlz

14666_wholemount_weak.wlz

14666_wholemount_possible.wlz

14666_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14666_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

spinal cord

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 12 15 16 weak expression: see section 09 10 14 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5401
Entity Detected:	Wdr17, WD repeat domain 17 ( MGI:1924662)
Sequence:	sense strand is shown >T5401 CCACGCGTCCGCACAAAATGTGTCAGAGCATCTAAATCATACGTCCAAGAATGCAAGAATGCGCTGACTG TTCTCCAGCTGACTTGATTAAAGTGCAAATATGGCTTGGATGACTTACGTTTCCCATTGGTTTGAGCAAG ATGATTGGTATGAAGGACTCCAAAGAGCAACCATGTCCCAGGTCAGGCAAGTGGGACTTCTGGCTGCTGG ATGTCAACCATGGAACAAAGATGTATGTGCTGCCAACGGAGATCGGTTTGCATACTGCGCAACTCTGGCC ATCTACATATACCAGCTGGATCACCGTTATAATGAATTCAAACTTCATGCAATTATGTCGGAACATAAAA AAACAATCACGGCAATTTCCTGGTGTCCACATAACCCTGACCTGTTTGCAAGCAGCAGTACCGATAACTT AGTGATCATTTGGAACGTGGCTGAGCAAAAAGTCATTGCTAAACTCGACAACATCAAAGAGACACCTGCC TGCCTCGGCTGGTGCTGGAACACACATGATGCTGTGGCATTCGTTTCCCAGAAGGGTCCTCTGCTCATTT GGACCATCTCAGGGCCAGATAGTGGAGTGTCTGTGCACAAAGAAGCCCATAGTTTTGCGTCTGACATTTG CATATTTAGGTGGCACACCCAAAAGAAAGGAAAAGTTGCATTTGGTCACATTGATGGAAGCATATCGATT TTTCAGCCTGATGAGGAGGACCCTGTTACAGCCCTGGAGTGGGACCCACTGTCCACAGATTATCTTCTGG TGGCTAATTTGCATTTTGGAATTCGTCTTCTGGACTCTGAATCCCTTTACTGCATCACAACGTTTAATCT TCCCAGTGCTGCAGTGTCTGTGCAGTGCTTGGCCTGGGTTCCCAGTGCTCCAGGCATGTTTATAACTGGA GATTCTCAAGTGGGCGTTTTACGCATTTGGAATGTTTCAAGAACAACACCTATTGATAATTTTAAATTAA AGAAAACAGGATTTCACTGCTTTCATGTAGTTAATTCTCCTCCAAAGAAAAAATTTTCCGTCCAGTCTCC AAACAAAAATCATTATATATCTTCAACAAGTGAAGCAGTTCCACCCCCAAATTTGACACAGAATCAAGCA TTTTCTCTTCCCCCTGGTCATGTCGTATGCTGTTTCCTGGATGGTGGAGTTGGACTTTATGACATGGGAG CCAAGAAGTGGGATTTTCTTAGAGAACTGGGACACGTGGAAACTATATTTGACTGCAAATTCAAACCTGA TGATCCAAATGTTTTAGCAACAGCTTCATTTGATGGCACTATAAAGGTGTGGGATATAAACACATTAACA GCAGTGTATACCTCTCCGGGTAACGAAGGGGTCATTTTTGCTCTTTCTTGGGCTCCAGGTGATTTAAATT GCATTGCTGGTGCAACTTCCAGAAATGGCGCTTTTATTTGGGATATTCAAAAGGGCAAAATAATACAGCG ATTTAATGAGCATGGAAAAAATGGAATATTCTATATTGCCTGGAGTCATAAAGATTCTAAACGAATAGCA ACTTGCAGTGGCGATGGTTTTTGCATTATTCGAACAGTCGATGGTAAACTACTGCACAAATACAAACACC CGGCTGCAGTGTTTGGCTGTGATTGGAGCCAAAACAACAAAGACATGATAGCCACTGGCTGTGAAGACAA AAACATCCGTGTCTTTTATGTAGCCACGAGCTCTAATCAGCCCTTGAAAGTGTTTAGCGGGCACACAGCA AGAGTGTTCCATGTTAAGTGGTCTCCTCTGAGGGAAGGAATTCTCTGCAGTGGTTCTGATGATGGCTCTG TTCGAATCTGGGATTATACTCAAGATGCTTGCGTAAGCACTCTTAATGGACACACTGCACCTGTGAGGGG GTTAATGTGGAATACTGAGATTCCATACCTACTAATCTCTGGAAGCTGGGACTATACTATCAAAGTGTGG GATACTCGAGGAGGAGTTTGCCTAGATACTGTGTATGATCACGGTGCAGACGTTTATGGTTTAACTTGCC ATCCAAGTCGTCCCTTCACGATGGCTTCCTGCTCCCGAGATTCTACAGTGAGACTCTGGTCATTAATACC TTTAATCACCCCTTTACAAATAAATATTCTGGCAGACAGATCTTGGGACGAAGTTATCGGAAACACTGAT AATGCGATACGTCAAGACTGTCCTCCTCTCCTCTGCGGCAAAGTGTCAAGAGACATTAAACAAGAAGTAG AAAAACTAACGTGCAACCCTCGGGCGAAAAAGCTAAGACTGTTTTCAGAATGTTTATCACCTCCAGGTGG CAGTGACAACTTGTGGAACTTGGTGTCCGTGATTAAAGGACAAGATGACAGCTTGCTCCCTCAGAACTAC TGCAAAGGAATGATGCATTTGAAACACCTGATCAAATTTAGAACCTCAGAAGCCCAAGAACTAACGACAA TCAAGATGTCTAAATTTGGTGGTGGTGTTGGCGCACCTACTAAGGAGAAAAAACTGAAGGAAGCTGCTGA AATCCACCTGAGATTAGGACAAATACAGAGATACTGTGAACTCATGGTCGAACTTGGAGAGTGGGACAAA GCCCTATCAGTTGCACCTGGGGTCTCTGTGAAATACTGGAGAAAGTTAATGCAGAGGAGAGCTGACCAGT TAATTCAGGAAGATAAGGATGATGTCATTCCATACTGTATAGCCACTGGGGATGTGAAAAAGCTCGTCAA TTTTTTTGTGTCCAGGGGTCAGCTTAAAGAAGCACTGCTTGTTGCACAGGCTGCCTGTGAGGGAAATATG CAAAATGCCCATGTTTCTACACCAAAAGGAGCTTCAAACTCTGATGATATCCACAAAGAAGACTTTAATG AACTCTTACACAAAGTCATAAATGAGCTGGCCGAATGGTATTTTCAGGATGGCCAAGCTGTGTTGGCTGC GTGCTGCCATCTTGCTGTAGATGATATTGAGCTTGCTATGGCATACCTGATCCGTGGGAATGAACTAGAG CTGGCGGTCTCAGTGGGCACTGTCCTAGGAGAACCCGCAGCCCCAGCAATGCATTATGCCCTGCAGCTAC TGGCGACAAAATGCATGATGATTCCAATGTGCTTCCCATCTGTTGTATACAGGAACCTGGCAGCTGATCT TCTCCTGATGACTCCTGGTAGCGAAGTCCACTTAGTAAAACTCTGTGCCTTCTACCCTGGGTGCATCGAA GAAATCAATGACCTTCACAAGAAGTGCAATCTACCCCCTGCAGAAGAATGCACTCAGTTGGCTGAAACAG CCCGGGCAGATGGCAATGTATTTGAAACCATAAAGTATTATTTACTAAGTCAAGAACCTGAAAAGGCTCT TCCTATTGGTATTGACTTTGTCAAAGGATGTATCAGTAACTCAAACTGGACTCTAGATACTGTTTACCCT GTGCTTGACCTGATGAGTTATATCCGTACTGAAAAATTACTCTTACATACGTGCACTGAGGCTCGAAATG AATTGCTTATCTTATGTGGTTACATCGGTGCCTTGCTGGCTATCAGGAGACAGTATCAAAGCATTGTTCC CTCGCTGTATGAGTACACGAGTCAGCTCTTAAAACGACGAGTGGTGTCAGTACCATTAAAAATTGAGCAT CTCTCTGAGGAGCTGGATGCATGGAGAGCTTGCACACAATCGACCAAACAATCATCAGAAGATTCTCCAT ATACACCCCCTTCTGACTCACAAAGAACAGTTTATGAAACTTTATTAAAGAGACTGAAAGAAGAGCCACT CAAAGGAATTACTGGACTAGATTACGTGACTGGATCAAATCTTCCAAGCCATTCAGACATTCACATCTCT TGTCTTACTGGACTAAAAATCCAGGGTCCTGTGTATTTCCTAGAAGATGGAAAATCAACTATTTCTTTGA ATGACGCTCTGATGTGGGCAAAGGTGAATCCATTCTCACCTTTAGGAACTGGAATACGACTCAATCCATT TTGACTGAATTTTTTTCTGCACACTTCCTAGTTTTGTAAAAAGGAAATTTGGGGTATTTATAACCTTCAT TTTGGTGGTCTAAGAATAATAGGTTGGGAGGTGGTTTGATACTTGGAAGACAGAGGGAATAATATTAAAT TTTGTTAATTTCAAAAATAAAATATATAATTCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5366098 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5366098 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14663 same embryo
	EMAGE:14661 same embryo
	EMAGE:14662 same embryo
	EMAGE:14665 same embryo
	EMAGE:14664 same embryo
	EurExpress:euxassay_005530 same experiment
	MGI:4829194 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source