Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:16818

Cd53 CD53 antigen ( MGI:88341)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16818 EMAGE:16818 EMAGE:16818 EMAGE:16818 EMAGE:16818
"Pseudo-wholemount" of euxassay_008611. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008611_01 euxassay_008611_02 euxassay_008611_03 euxassay_008611_04
EMAGE:16818 EMAGE:16818 EMAGE:16818 EMAGE:16818 EMAGE:16818
euxassay_008611_05 euxassay_008611_06 euxassay_008611_07 euxassay_008611_08 euxassay_008611_09
EMAGE:16818 EMAGE:16818 EMAGE:16818 EMAGE:16818 EMAGE:16818
euxassay_008611_10 euxassay_008611_11 euxassay_008611_12 euxassay_008611_13 euxassay_008611_14
EMAGE:16818 EMAGE:16818 EMAGE:16818 EMAGE:16818 EMAGE:16818
euxassay_008611_15 euxassay_008611_16 euxassay_008611_17 euxassay_008611_18 euxassay_008611_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16818Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16818_wholemount_strong.wlz
16818_wholemount_moderate.wlz
16818_wholemount_weak.wlz
16818_wholemount_possible.wlz
16818_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16818_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
strong strong
regionalstrong expression: see section 08 09 10 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2801
Entity Detected:Cd53, CD53 antigen ( MGI:88341)
Sequence:sense strand is shown

>T2801
GGCCTCGAGCCAGATTCGGCACGAGGGGNCACCTAAAAGATAATCAGTGAATTCTACCTGAAGTACTGAG
GGACACTGCCTTCAAAAGGGCATACTATCCCAGCATGGGCATGAGCAGCCTGAAATTGCTGAAATATGTT
CTGTTTATCTTTAACTTGCTTTTTTGGGTCTGTGGCTGTTGCATTTTGGGCTTTGGCATCTATTTCCTGG
TCCAAAATACCTATGGAGTACTCTTCCGTAACCTTCCCTTCCTGACACTTGGCAACATTCTGGTCATTGT
GGGATCCATTATCATGGTAGTTGCCTTCTTGGGTTGCATGGGCTCAATCAAGGAAAATAAGTGCCTGCTT
ATGTCGTTCTTTGTTCTGCTGCTGATTATTCTCCTTGCTGAGGTGACCATAGCCATCCTGCTCTTTGTGT
ATGAACAAAAACTCAACACTTTAGTGGCTGAGGGTCTGAATGACAGCATCCAACATTATCACTCTGACAA
CAGCACTATGAAGGCATGGGACTTCATCCAGACACAACTGCAGTGTTGTGGTGTAAATGGCTCAAGTGAT
TGGACCAGTGGTCCACCATC
Notes:The probe template was PCR amplified from IMAGE:1516010 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1516010 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16821 same embryo
 EMAGE:16819 same embryo
 EMAGE:16820 same embryo
 EMAGE:16817 same embryo
 EMAGE:16822 same embryo
 EurExpress:euxassay_008611 same experiment
 MGI:4823737 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS