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EMAGE:16820

Tmem55a transmembrane protein 55A ( MGI:1919769)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16820 EMAGE:16820 EMAGE:16820 EMAGE:16820 EMAGE:16820
euxassay_008648_01 euxassay_008648_02 euxassay_008648_03 euxassay_008648_04 euxassay_008648_05
EMAGE:16820 EMAGE:16820 EMAGE:16820 EMAGE:16820 EMAGE:16820
euxassay_008648_06 euxassay_008648_07 euxassay_008648_08 euxassay_008648_09 euxassay_008648_10
EMAGE:16820 EMAGE:16820 EMAGE:16820 EMAGE:16820 EMAGE:16820
euxassay_008648_11 euxassay_008648_12 euxassay_008648_13 euxassay_008648_14 euxassay_008648_15
EMAGE:16820 EMAGE:16820 EMAGE:16820 EMAGE:16820 EMAGE:16820
euxassay_008648_16 euxassay_008648_17 euxassay_008648_18 euxassay_008648_19 euxassay_008648_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16820Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16820_wholemount_strong.wlz
16820_wholemount_moderate.wlz
16820_wholemount_weak.wlz
16820_wholemount_possible.wlz
16820_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16820_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1272
Entity Detected:Tmem55a, transmembrane protein 55A ( MGI:1919769)
Sequence:sense strand is shown

>T1272
TCGAGNCTGTTGGCCTACTGGATTGCTGGCCACCACCTCCTCCCTAGCCGCAGCCCAGCTCAACCCGCGG
CCCGCCGCGCTGTCGCTGCAGTCATGGCTGCTGACGGGGTGGACGAACGTTCGCCTCTGCTGTCAGCATC
CCATTCGGGAAATGTCACTCCCACAGCCCCGCCGTACTTGCAGGAAAGCAGCCCCAGAGCTGAACTCCCA
CCTCCGTATACAGCCATCGCCAGTCCAGGAACAAGTGGTATTCCCGTGATCAACTGTCGTGTGTGCCAAT
CTCTAATCAACCTGGATGGTAAACTTCACCAGCATGTGGTTAAGTGCACAGTTTGCAATGAAGCTACGCC
AATCAAAACCCCCCCAACAGGGAAGAAATATGTTAGATGCCCTTGTAATTGTCTACTCATTTGTAAGGAT
ACATCTCGGCGAATAGGATGTCCGAGACCCAACTGTCGACGCATAATTAACCTTGGCCCCGTAATGCTCA
TTTCTGAAGAGCAACCAGCCCAACCTGCATTGCCGATCCAGCCAGAAGGCACAAGGGTAGTGTGCGGGCA
CTGCGGGAACACATTCCTGTGG
Notes:The probe template was PCR amplified from IMAGE:2225700 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2225700 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16818 same embryo
 EMAGE:16821 same embryo
 EMAGE:16819 same embryo
 EMAGE:16817 same embryo
 EMAGE:16822 same embryo
 EurExpress:euxassay_008648 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS