EMAGE mouse gene expression spatial database

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EMAGE:17108

Ptges prostaglandin E synthase ( MGI:1927593)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001497. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001497_01	euxassay_001497_02	euxassay_001497_03	euxassay_001497_04

euxassay_001497_05	euxassay_001497_06	euxassay_001497_07	euxassay_001497_08	euxassay_001497_09

euxassay_001497_10	euxassay_001497_11	euxassay_001497_12	euxassay_001497_13	euxassay_001497_14

euxassay_001497_15	euxassay_001497_16	euxassay_001497_17	euxassay_001497_18	euxassay_001497_19

euxassay_001497_20	euxassay_001497_21	euxassay_001497_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17108_wholemount_strong.wlz

17108_wholemount_moderate.wlz

17108_wholemount_weak.wlz

17108_wholemount_possible.wlz

17108_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17108_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 10

bladder

moderate

regional

moderate expression: see section 10 11 12

genital tubercle of male

moderate

regional

moderate expression: see section 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4488
Entity Detected:	Ptges, prostaglandin E synthase ( MGI:1927593)
Sequence:	sense strand is shown >T4488 CGGTGTCCCCGAGTTGAAGTCCAGGCCGGCTAGCCGAGATGCCTTCCCCGGGCCTGGTGATGGAGAGCGG CCAGGTGCTCCCGGCCTTTCTGCTCTGCAGCACACTGCTGGTCATCAAGATGTACGCGGTGGCTGTCATC ACAGGCCAGATGAGGCTGCGGAAGAAGGCTTTTGCCAACCCCGAGGATGCGCTGAAACGTGGAGGCCTCC AGTATTACAGGAGTGACCCAGATGTGGAGCGCTGCCTCAGAGCCCACCGCAACGACATGGAGACAATCTA TCCTTTCCTCTTCCTCGGCTTCGTGTACTCATTCCTGGGACCCAACCCTCTGATCGCCTGGATACATTTC CTCGTTGTCCTCACAGGCCGTGTGGTACACACCGTGGCCTACCTGGGCAAGCTGAACCCACGCCTTCGCT CCGGGGCCTATGTCCTGGCCCAGTTCTCCTGTTTCTCCATGGCCCTGCAGATCCTCTGGGAGGTGGCCCA CCATCTGTGACCAGCAGCTGAAGCCTCCTCAGCTACCACAGTGGGGACAGAGCCATGAAGGCTGAACTTG CGGACTTGGTGTCTCTTCTAGAATAGGGACGGGGTCTGGTCCTGGGGTCCTGGGTAACCTGGGCGAGTGG ATCTCAGGGCCCAATGTGCACATGTGTGTTTCTTAGCCTTTTGGGATGCTAGATGAAGTCCCATTTGGAG CCACTTACAGATGAGCTGTCAAGATTTGAGAGGACATCCTTCACCTCACAAAATGCCTTAAACATCACAC CACACTCCCTCTTAACCATAAAATAAAGGACAGGATTGACCAACAGAGCCATCATCCCATATAGGACTAA GAATGAAGGCTTCTCAGACCTACCTACAATTCTGGCCCAGCCCAAGGAGAGCCACCTGGCTGGTATGTGT CCGAGGACATCCTCACAAGAGGCCCAGCAGTGGCCAGCTCCATGTGGAGGTAGAGTGCTTGCTATTTTTA GAGGTGGGCAGGTCAGAGCATCCCTGTCCCCTGTTGATACCACCGTCCTTTGAGTGCCTTAGGTAGCACA GGCTCAGGGGGGTGAGAATGTCCACAGCTCAGCCTCCTTGTGCATGGCTTTGGCTCCTCCAAAGACGGAA AGGATGGGCTTTGACCAACCCCTGGAAGGGAACTTTGGCTTCTTCAGCATCTGTGAGGTTTGAAGATGCC AAATGTCTACTCCTGTGGAAAATCTCACTTGTGTGTGTGCAGTGTCCCTCCAGAGTCCTGAGACCTGTTT AGGTTTAGTCATTAAACAGCAGAGTGTGACCTGTGAATGTTCGGTGTCTAAGACCCATGCTGTGCCAGCC GGCCTGGTACCTATGGGAGTTTTCACGTTCCGGTGTGTGTATGTTTTACTTTTATGAGACAAGGTCTCAC TTAGCCCGAGTTGGCCTCAAACTTACTCTACAGTCAGGACAACCTTGAGCTGACAGCCTACCTGTGCCTC CTAAGTGCTGGGATCGCCGGCCTGCACCACCATAGTGCCAAGCCAGTACAGGCAACTTCCACCAGGCTGC TCCAGAGAAGTCCCTGACAAGATGGTGTTTGGCTTGGGATCCAGAGATGTCCAAATCCTGTCTTCCATGT GGCGATTCTTTCTGCAGCTCTGCCACCTTGTAGGGTGCTGGTTTTCTGCTCTACAGACGGAGACGGAAGT GACTACCTGAAGGAAGGCATAGGGTGTGCTCCAGAACCTGGGACATTCAGGCTCCCAGGCTTCCCACACA AGCCTAGCCACACCACTTCTCCTCCCAGCGGGGCAAGCCCTGTGCCCCAGACCTGCTCTATGGGGTTGGG GAGGGGGTTTTACTGGAAAGCCCTGCTGGCCTAGGCTTCAGCCTCACACAGCTGGTGTTCTGGGAGTGAA TAGCACTGCCCTCTGGTGGTGAGTTGAAACCTTTGCTCTTGGAGTGCTCATCTGAGAAAACAAAAACTGT AGAATGGGGCCCCTCTTGAATGCCAGCTGTCGCTGAACATTAGGCTGGATGCGACCTTCATCTGTCATTG TCACCCCAACCCCAATGCCCCAGTTAATACTTAAATTTTAGATAAGACCAGGTGAATTCAGACAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4950950 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4950950 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17106 same embryo
	EMAGE:17107 same embryo
	EMAGE:17111 same embryo
	EMAGE:17109 same embryo
	EMAGE:17110 same embryo
	EurExpress:euxassay_001497 same experiment
	MGI:4827506 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source