EMAGE mouse gene expression spatial database

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EMAGE:17110

Cirh1a cirrhosis, autosomal recessive 1A (human) ( MGI:1096573)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001499. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001499_01	euxassay_001499_02	euxassay_001499_03	euxassay_001499_04

euxassay_001499_05	euxassay_001499_06	euxassay_001499_07	euxassay_001499_08	euxassay_001499_09

euxassay_001499_10	euxassay_001499_11	euxassay_001499_12	euxassay_001499_13	euxassay_001499_14

euxassay_001499_15	euxassay_001499_16	euxassay_001499_17	euxassay_001499_18	euxassay_001499_19

euxassay_001499_20	euxassay_001499_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17110_wholemount_strong.wlz

17110_wholemount_moderate.wlz

17110_wholemount_weak.wlz

17110_wholemount_possible.wlz

17110_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17110_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 08 09 11 12

telencephalon ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 11 12 13 14 15 16 17 18 19

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 07 13

cervical ganglion

moderate

regional

moderate expression: see section 06 14

thoracic ganglion

moderate

regional

moderate expression: see section 08 09 11 12

renal cortex

moderate

regional

moderate expression: see section 05 06 07 14 15 16

tail dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4473
Entity Detected:	Cirh1a, cirrhosis, autosomal recessive 1A (human) ( MGI:1096573)
Sequence:	sense strand is shown >T4473 CGGCAAAAAAGTCGGATTCTCAGTCTCTGCTGGCACCCTGCGGGTACCCACGTCGCAGCTGGTTCCTTAG ACTACATTAGCGTGTTTGATGTCAAATCAGGGAGCATAATTCGTAAGATGGTTTTAGATAGGCAACACCT GGGAGTGACTAAGTCCAGGTGCATAGTGTGGGGTGTTGCCTTCTTGTCCGACGGCACTGTCATAAGTGTG GACTCTGTTGGAAAGGTGCAGTTGTGGGACTCTGCTACTGGGACACTCGTTAAGAGTCATCTCGTCGCAA ATGCTGATGTGCAGTCTATTGCTGTTGCTGATCAAGAAGACAGTTTTGTGGTGGGCACCGCCGAGGGCAC GGTATTCCATTTCCAGCTGGTGTCCATGACTTCCAACAGCAGTGAGAAGCAGTGGGTGCGGACGAAACCG TTCCAGCATCACACTCATGACGTGCGTGCTGTGGCCCACAGCCCAACAGCCCTGATCTCAGGAGGCACCG ACACCCACCTGGTTATTCGGCCTCTCATGGAGAGAGTGGAGGTAAAGAATTACGATGCTGCTCTCCGAAA GATCACGTTCCCCCATCGGCGTCTCATTTCCTGTTCAAAAAGAAGGCAGCTTCTCCTTTTCCAGTTCGCG CATCACTTGGAACTGTGGAGACTAGGATCCACATCTGCAACAGGCAAGAACGGAGATACTCTTCCACTCT CTAAAAACGCAGATCATCTCCTGCACCTCAAGACAAAGGGCCCTGAGAACATTATCTGCAGCTGTGTCTC CCCGTGTGGAAGCTGGATAGCCTATTCTACAGCGTCTCGCTTTTTTCTCTATCGATTGAAATATGAACGC GATAACATAAGCCTCCAAAGAGTTTCCAAATTGCCATCATTCCTGCGCTCTGCCCTTCATATTCTGTTTT CTGAAGATTCAACAAAGCTCCTTGTGGCATCCAATCAAGGGTCTCTGCATATTGTTCATCTGTCGGAAGG AAGCTTCAAGCACTTGCATACTTTCCAGCCGCAGTCAGGTACAGTGGAGGCCATGTGTCTTTTGGCAGTC AGTCCAGATGGGAACTGGCTAGCTGCATCGGGTACCAGTGCTGGAGTCCACGTATATGATCTGCATCATC TAAAGCTTCACTGCACAGTGCCTGCTTACAACTTCCCAGTGACCGCTCTGGCCATCGCCCCCAATACCAA CAACCTGGTCATTGCTCATTCTGACCAGCAGGTATTTGAATTCAGCATCCCAGAAAAGCAGTATACAGAG TGGAGCCGCTCTCTCCAGAAGCAAGGATTTCACCAGCTGTGGCTCCAAAGGGATACTCCCATCACACACA TCAGCTTTCATCCCAAGAGACCAATGCACATCCTCCTCCATGATGCCTACATGTTCTGCATCATTGACAA GTCATTGCCACTTCCAAATGAGAAAACTGTGCTTTATAATCCACTTCCTCCGAAAAATGAATCAGATGTC TTCTTGAGGCGGACAACCCATGGGTTTAAAATGTCAAAGATATATAAGCCCTTGCTCTTTATGGATCTTT TGGATGAAAGGACACTTGTGGCAGTAGAGCGACCCCTGGACGACATCATTGCTCAACTCCCACCACCCAT CAAAAAGAAGAAATTTGGAACTTAAAACAGGGCACTGTCTGTGTCCTTTCTTGAACTGTCTATCCTGTTG TTTTGTTTGTTTGTTTGTTTGTTTTTTCCCAAATCATGATAATAAAACAAAGTTATTCTTTAGGACTAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4948207 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4948207 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17106 same embryo
	EMAGE:17107 same embryo
	EMAGE:17111 same embryo
	EMAGE:17108 same embryo
	EMAGE:17109 same embryo
	EurExpress:euxassay_001499 same experiment
	MGI:4823879 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source