EMAGE mouse gene expression spatial database

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EMAGE:17285

Atf1 activating transcription factor 1 ( MGI:1298366)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003681. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003681_01	euxassay_003681_02	euxassay_003681_03	euxassay_003681_04

euxassay_003681_05	euxassay_003681_06	euxassay_003681_07	euxassay_003681_08	euxassay_003681_09

euxassay_003681_10	euxassay_003681_11	euxassay_003681_12	euxassay_003681_13	euxassay_003681_14

euxassay_003681_15	euxassay_003681_16	euxassay_003681_17	euxassay_003681_18	euxassay_003681_19

euxassay_003681_20	euxassay_003681_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17285_wholemount_strong.wlz

17285_wholemount_moderate.wlz

17285_wholemount_weak.wlz

17285_wholemount_possible.wlz

17285_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17285_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 08 09 10 weak expression: see section 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5700
Entity Detected:	Atf1, activating transcription factor 1 ( MGI:1298366)
Sequence:	sense strand is shown >T5700 GCCGCGACGGTTGGCTCTCGAGTTACGCTCTCCTCCCGCACCCCGCCGACCCCTGGGACGCGCGCCACCT CGCTGCGGTGCGCGGCTGGCAAGTGAGGAGTAAAGTCGGCGCCGCCTGGGACGGGGGAGTGGAGCTTCCT GCCCCGGGCAGCGGCCAGGCGGCTCCGCGGTTGATTATGGAAGATTCCCACAAGAGTAACACGACAGAGA CTGCATCTCAGCCTGGTTCAACCGTTGCGGGGCCTCACGTTTCTCAGATAGTACATCAGGTGTCATCGTT ATCAGAAAGTGAGGAGTCTCAGGACTCATCTGACAGCATAGGCTCCTCACAGAAAGCTCACGGGATCCTG GCCCGGCGCCCATCCTACAGGAAAATTTTGAAAGACCTCTCTTCTGAAGATACACGGGGCAGAAAAGGGG AAGGAGAAAATCCCAGCATTTCTGCCATCACGTCTATGTCTGTTCCAGCCCCCATCTATCAGACGAGCAG CGGACAGTACATTGCCATTGCCCCAAACGGAGCCTTACATTTGGCCAGTCCAAGCACGGATGGAGTGCAG GCACTGCAGACATTAACCATGACAAATTCAAGCAGTACTCAGCAGGGGACGATCCTCCAGTATGCACAGA CCTCTGACGGACAGCAGATACTCGTCCCGAGCAACCAGGTGGTTGTACAGACTGCATCAGGAGATATGCA GACCTACCAGATCCGTACCACGCCATCTGCCACGTCTCTCCCACAGACCGTGGTGATGACTTCTCCTGTG ACTCTTACATCTCAGACAACAAAGACAGATGACCCTCAACTAAGAAGAGAAATACGACTGATGAAAAACA GAGAAGCTGCCCGAGAGTGCCGCCGGAAGAAGAAGGAGTACGTGAAGTGCCTGGAGAACCGTGTTGCTGT TCTGGAAAATCAAAATAAAACTCTAATAGAGGAGCTAAAGACTTTGAAGGACCTTTATTCTCATAAAAGT GTTTGATTCTTTAAAGAGAAAGTATTTTTTGTGGACTTTAAAGCTTAAAAATTAGGTGGATTTCTTTTTA GTGGAGTTCTATAAATTCAAAGGTCAAAGAAGCTACTTTGTATTAGGTTTTCACAAACAGACATGATGGA GGGAGAATGGAGATGACCTGCAGGAAGCTACTGTCTCAATTGGAAGCTCTGAAAAGATAGAGTGCTCAAG GAATGGACTTTCAGCAGTTTAAATAGAAATAATGGTCAGCAACGAAAATGGCAGGCGACGTTAGTGAAGC AGTCCGTTCCTGCTACGTCTGTATTTCTCTGTTCCCTAAAACTTGCCTTTGTGTGTGTGCGCGTGTGTGT ATGTGTGTGTGCGTGCGTGCCTCATTTCTGCCAATAAATTTACAAATGTAAAAGCATAAAGCGTTACTAA GTATGTAAATTATGTGGTCTGATTGTGTCGTAATGTCAGGTATGTTAAATGAGTTTTTAGCAGTTAGTTT GGTTGGACTCGATTGGTTTGGAGATCTCCTAAATGGCGTGGAGACACATCAGCCTGTGGTGGAGGTGGTT TGTGACTTTGCTTTGTCAGAATGGAAAGAGAACTGTCTGCAAGTTTGACAGTTTAATAGTTTGTTTTTTG CTTCCTCTGATAAGGGCAAGTACATAATAGATTGGTTTTGTTTTTTTTGTTTTTTGATTCTCTAATCCTG TGTGCAGTTGAAGATCATTCTCTTGCGCTTTAACAGAATGAAGTGTTTACAAAGGCCCTCAAATGTTTTA AATAACCTGAATATGCACACCAAAGCTTTCCTGAGGGGTTTTATAATCATCTTACCGTAAGGTTTGTCAG GTTCTAAAACAGTTACTGAGGCAATCTTACATTGAGACTATTGTGTAATGTAGCCCATGGTACCTTTATA AAAGAAGTGACTGCCGATATATTTTTATAGTGAATCTTTATAAATTCTAATGTTGAGTTTTTAATGATTA TTTTAAATGTTTATATAGTTTGTTTAGCAAAAAAAATTTTGTATCTCAAAGTATCATTTTTATATTATGA GAAGAGTAAAGTTTTCAAATTGGCTGATAATTGTATGTTTTATATAAAGTTTTATCTAAAGTTTCAGCTG TGGTCAGTACACCAGCATTTAACCTCTGTATTCTAATCTGTGTTCACTTGGAAACTGCAGAACTGTTGTG TGCCTACAGAGTATGTTTCATATTGTCTGTGAAATTAAAAAACATTTGATAAGAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2646620 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2646620 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17288 same embryo
	EMAGE:17283 same embryo
	EMAGE:17286 same embryo
	EMAGE:17284 same embryo
	EMAGE:17287 same embryo
	EurExpress:euxassay_003681 same experiment
	MGI:4823290 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source