EMAGE mouse gene expression spatial database

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EMAGE:17384

Mslnl mesothelin-like ( MGI:3607710)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003737. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003737_01	euxassay_003737_02	euxassay_003737_03	euxassay_003737_04

euxassay_003737_05	euxassay_003737_06	euxassay_003737_07	euxassay_003737_08	euxassay_003737_09

euxassay_003737_10	euxassay_003737_11	euxassay_003737_12	euxassay_003737_13	euxassay_003737_14

euxassay_003737_15	euxassay_003737_16	euxassay_003737_17	euxassay_003737_18	euxassay_003737_19

euxassay_003737_20	euxassay_003737_21	euxassay_003737_22	euxassay_003737_23	euxassay_003737_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17384_wholemount_strong.wlz

17384_wholemount_moderate.wlz

17384_wholemount_weak.wlz

17384_wholemount_possible.wlz

17384_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17384_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19

heart

moderate

spotted

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 not examined expression: see section 01

lung

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7767
Entity Detected:	Mslnl, mesothelin-like ( MGI:3607710)
Sequence:	sense strand is shown >T7767 CTGGACCTACAACCCACAGCCTTCGGGCTTCCCTGCTTTGAGACATTTCAGCCAGGATGAGCAGAACTCT CCGACCGTCAGCTATGGGTTCCAGAGTGGGTGCCCTGGCAAGTCCTGGTCTCGCCCTCCTCCTGTCCCTC ACCGCCCACTGCTCGGGTCCTCAGGCTAAAGGCTTGCCCAAGGGAGGACTGAATGCAAGTGAAGCCAACA CATGGGTCAGGAACAACACCTCCCTCCTGCAAAACTTCTGGTGCTTGCCAGCCAGCCAGCTGCCCCGAGA ACAGCTCTCCAGTTTAATCAGAAGCCTGGCTTCACAGAGGGTTGCACTCAAAGCCTGGCAGCTCAGCTGC TTGGCCAACCTCGCTGCACAGCTGGGTCTCCAGGATGACTTCGAATTCCACCCACCCAACCTTCTACTTT TCTACGATCTGAGCCAGGTGGGGGACACTAACTGCCGGGCCTTCATTCACCATGCTGCCCAAGGGGACAC AGAACTGCTCACCAACTTGCCCAACCAGAGAGTTGCCCTGCAGCGCACAGCCTTGGCCTGTCTGGGCGGA CCCCACCTACAGCTCAGTGCTTCAGACCTGTGGCTTCTTGGGGTCCTGGTGTGTGACATGGAGGCAGCAC AAATTGTGACTGCAGACCCCAGTGTGCTGAGGAACCTGCTTCGCTGCCCAAGGCTTACTGTCATGCAGAC TGCGGCCCTTAACACCCTGCTGGCCAGTGGGAAGACGCAAATCGGGCCTCCTGGCTCCTGGAACCTGCAG GGGCTACAGGCCCTCGGACTTCTGGCCACTTACATCAGCCCCCACCTGTGGGAGAAGGTGCAGGAGGCTG TGGGCTTGGACTTCTTCCGCAGTGTGGTGGCTGCTTGCCGGGCAGGCCAACTGAGCAGGCACGATGCCAG ACGCTTCGTTGACAACTTCCTAGAGTCCAAGGCCACTTCAGTGTCCTCAAGGCCCAAGCGGCGCACAGGG AGATCCTGTGTCCGTGGCAACATCACAGCAGCCACCCTGCACGATGACCTCTTCCTGGTGCACTATGACT GCACCCAGCTGGAATCCTGCCTAGGCACACGCGTGCTCAGGGCCAACCTGGACCCCTTGCTCCAACACCC GCTGCCTGCAGAGTGCCAGCGCGTTGTCAAAGCCAAGCTTACTCAGATTTACCCACATGGCATCCCTGAG GACCAGCTGCACCTCATCCCCTCGCTGGTCTACCTCTACTCCCTCGCTGAAATAGGCCAGTGGAACATCA CATCCGGGGACACAGTGATGATCCTGCTGGCCTCAGATGCGGCTCTGGACAACCAGACGGAGGCAGTTCT ACAGAGGTTCTTGGACCACAATGGCAAGGTCACTGGAGCTCTTCTGGTGGCCATCGGGGGTTCCCGTCTC TGCTGGATGAGCCTCAAGCAGCTTCAGTTTATCCAGCCCTCAGAGTTCCGGCTGGCCGGAGCTCCAGACA TCTCTCCCTGCCCCCAGAGTCGGAAGGATGCGCTCTTTGTCAAGGCTCATGAGGTCTTTAGGAACACCAG TAATGTGGGAGAATATTACTACTTAATCCGCCCCTACCTCGGTGGTGCCCCTTTGGAGGAACTTCAATAC CTGGCACAGGCCAACATCTCCATGGACATTGACACGTTCACCAACCTCAACCCCCTGACACTGAAGAGCA TGGGAGTGGACAGTGTGAGGAATCTGCTGGGCCAGAACCTGGGAGATCTGCGGAAGGCCCGCAACCACCC AAATGTCATCCTGTGGATGCACAGCCACAACATGACAGACCTGTCTGATATGGGCCTATACACCAGCCCC ACACAGGCCTATGTGGCCAACAGGCCCCCCAACACTGCCTACCCACCATCCAGTTTGATCCACTCCTTGG ATCCGCCAGGCAATGATGGGGTTTCCCACACCTCAGGAAGTCCTCCAGTTCATCTGGGGTACCTGTCACT CGCTGTGGCTCTGCCTTCCAGCCTTCTGTGGCTGCTACTGTGTCAGTTACCTTCGGGACAAATGGCCACT GCTCACAGGACACTAGGGCCAATGGCACTGGCCCAGGGCAGTTGGACCCCTGAACATCAGATTCCAGAGA AACGGAGCTGCTAGATGGGACAGGACACCTGCCTAGGCTGAAGTACATGTCCCCAGCCCCAGGGGACCTG CTGCTATGTCCCCATCCTAGGAGGCTGTCTCTGACTCCCAGGATCTGAGCCCATTCTGCCCAACTAAAAG GACAAATTGGTAGGAGGAGACAGAGTCCAGATCTTCTGGCCTATTCCAAGATAGATGGGACTCGGTGTTA CTTGAATGTCACTTGCCCTCGATGGCGGATCTGAATAAAGCACAAGGCCATTGCAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6312790 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6312790 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17385 same embryo
	EMAGE:17389 same embryo
	EMAGE:17388 same embryo
	EMAGE:17387 same embryo
	EMAGE:17386 same embryo
	EurExpress:euxassay_003737 same experiment
	MGI:4826469 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source