EMAGE mouse gene expression spatial database

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EMAGE:17385

Ccdc65 coiled-coil domain containing 65 ( MGI:2146001)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003694. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003694_01	euxassay_003694_02	euxassay_003694_03	euxassay_003694_04

euxassay_003694_05	euxassay_003694_06	euxassay_003694_07	euxassay_003694_08	euxassay_003694_09

euxassay_003694_10	euxassay_003694_11	euxassay_003694_12	euxassay_003694_13	euxassay_003694_14

euxassay_003694_15	euxassay_003694_16	euxassay_003694_17	euxassay_003694_18	euxassay_003694_19

euxassay_003694_20	euxassay_003694_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17385_wholemount_strong.wlz

17385_wholemount_moderate.wlz

17385_wholemount_weak.wlz

17385_wholemount_possible.wlz

17385_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17385_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon roof plate

moderate

homogeneous

moderate expression: see section 13

lateral ventricle choroid plexus

strong

regional

strong expression: see section 07 08 09 10 11 17 19 20 moderate expression: see section 12 13 14 15 16 18

4th ventricle lateral recess

strong

regional

strong expression: see section 03 04 05 06 07 09 10 11 19 20 21 moderate expression: see section 08 13 14 15 16 17 18

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19 weak expression: see section 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7761
Entity Detected:	Ccdc65, coiled-coil domain containing 65 ( MGI:2146001)
Sequence:	sense strand is shown >T7761 CTGTTCTCCAGCTGGGAGTCGGGTGTCGCCTGAGCACTCTTGGCGTGGAGGACAGAGGTCTAAGGCCCCA TTACCTTAGCTCCAGAAGGTCCCTCCCTGTCTATGGGAAGTTTTTCTTTCTAATTCCTCACAAGTGGCTC GTAGCAGCAGGCAGAGGCTCCTTGCCCGGACCCAGTCCGCAGCCCAGCCATGTCTAAAAAAGGAAAAAAG CCCAAGTTGCCCAAGGCACCCCTGTCCGAGGAAGATCAGCTGCTTATATTTCAGCAAAAGATGCTGGCTG ACGAAGAGGCAGCCAAGAAGAAAGAGAGACTCCTCACCCAGTTCTTGAAGGACAAGTTGGCCAAAGAAGA ACACAACAGCGCTCTAAACCTTAATAAGATTAACACGCAGTGGAGAACCATCCTCAGGGAGGTGAAGACC AGAGAGCTTCACCAAGACATCGAGATCCTCAGCCAGACCTTTGAACGCGTGGTGGACTGTAAGGACAGCG TCATCAAGTCTCTGGCTAAGGACTTGACTGAAGCTGAGGAGCAGTATGCCCACGCTCTGCGTAGTCACTT GCACAACATTGACCAGCTCTTGACCCTGCAACGACGCCGGCTCGGCCTCCTGGAGGAAAATTACAACATG GAGCTCGAGGTCCTGACCAAAGAGTTTGAGACGGAAAGGAAATTGATCATTGACCATCATGAGAAGGAGA TGCACTACCTACAGGACGTCTTCATGGCCATGGAGCAGAACTACATAGATTCCGAGTATGAGAGCAAGCT GGAGTTCCAGAGCATGTGGGATGACCTTAAAAACAAGAATTTAGAAGAGAAGCACTTTCTTCGCCTACAA CTGGAGAACATAGTGGAAGATCTGTGGCGGAGGTTCCAGGATGCACTCAAGAATTACACCGACGCCACGG AGGACCGGAAGATCGCCTTTGAGTACCTGAAGGTGAAGGATGAGAAAAGCTCCAAAGAGATTGAGACGCA GATGAAAAAGATCCAGAAGCTGCAGGAGACCATAGGTATCCTGAAAGGGAAGATCGTAGCCCACAGCCGC GAAGGGGAATGGCAGAATCAGTGCATCCGGAATAACAAGGAGCTGGTCCACGTGCAGCTTCGAAAACTTA AGGTCCAGAGGACCCAGGCTCGGACCCTATCTCAGGAGAACTTAGTCAAACTCACCCTGGAAAGTAATGC CACCCTCAAGGCCCTGAAGAAGGTCGTGGAAAAGGGTGAAAAGATCCTTAAGCTTGCTGAAATATGTAGG AAATTCGAAACCGAGGAAGAAAAGGTGCTGCCTTTCTACTCTTCAGTGCTGACTCCCGAGGAGCAGGAAG AGGCCAAGCTCCAGAACCCGGAGGATATCACAGAGGACCTTGCCAAGATTATGATGGACTACGCGGGGAT GGAGAACTTCTGGAAGAGATACAACAAGGTGAAACTGGAGGTCCTGAGTCTCCAGCACAGGCGGCTGCAG CTGCTGGATATCAGTAGCAAGCTGCGGGAGATGCTCAAACAGTACTTGGATGGCATCTCCGTGAGCGACG AAGTGCTGAGCCACCTCAACCCGCTCTTCGTAGTAAACCACAGAAGCAACCTACCCCAGCTGCCCCCACC CTCGGCCCAGCCTGTCTACAACGTCATCGAAGCCGCCCACATTGCCTCCCATATCCTGTGACCGGAGAAG GAGACTTCTTTCCTGACCCCTGAAAGGAGGCTTTCTCCTTTTTCCTTTTCTTTTGTGAAACCAGCGTGCT TTGCTCTCATGCTCAGATGGACAGGCACACTCAGACAGACAGACAGACAGACGCTCCAGCTGCTTACTAC TTACCACCACTACCCTTACAGTGTAAACGATGACTTCTTTTTTTTTTTAAAGAGATAAATAAATAAAAGT AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6312435 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6312435 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17389 same embryo
	EMAGE:17388 same embryo
	EMAGE:17384 same embryo
	EMAGE:17387 same embryo
	EMAGE:17386 same embryo
	EurExpress:euxassay_003694 same experiment
	MGI:4823682 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source