EMAGE mouse gene expression spatial database

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EMAGE:17430

Wdr3 WD repeat domain 3 ( MGI:2443143)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003872. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003872_01	euxassay_003872_02	euxassay_003872_03	euxassay_003872_04

euxassay_003872_05	euxassay_003872_06	euxassay_003872_07	euxassay_003872_08	euxassay_003872_09

euxassay_003872_10	euxassay_003872_11	euxassay_003872_12	euxassay_003872_13	euxassay_003872_14

euxassay_003872_15	euxassay_003872_16	euxassay_003872_17	euxassay_003872_18	euxassay_003872_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17430_wholemount_strong.wlz

17430_wholemount_moderate.wlz

17430_wholemount_weak.wlz

17430_wholemount_possible.wlz

17430_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17430_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7816
Entity Detected:	Wdr3, WD repeat domain 3 ( MGI:2443143)
Sequence:	sense strand is shown >T7816 GGGTCTCGTAGGCAGCGCTGTCTGCTGGAGCCTCGTGGAGAGTGAGATTGCCTTCCCGGTGTCATCAGAC AGCCCAGCATGGGGCTTACTAAACAGTATCTGCGCTATGTGGCTAGTGCTGTCTTTGGCTTGATTGGCAG CCAAAAAGGTAACATTGTTTTTGTGACACTCCGTGGTGAGAAAGGACGCTATGTGGCAGTACCCGCCTGT GAACACGTTTTTATCTGGGACTTAAGGAAAGGCGAGAAGATCCTTATCCTTCAGGGGAATAAACAAGAAG TCACTTGCTTGTGTCCTTCCCCAGATGGGCTGCATCTAGCTGTTGGCTATGAGGATGGAGCCATCCGAAT CTTCAGCCTCCTCAGTGGTGAAGGAAACATAACCTTCAATGGACACAAAGCAGCTGTCACCAGCTTAAAG TACGATCAGCTAGGAGGCAGGCTGGCTTCTGGGTCTAAGGATACAGATGTCATTATTTGGGATGTGATCA ATGAAAGTGGCCTCTACCGTCTAAAGGGACACAAGGATGCTGTCACACAAGCTTTGTTTCTCCGAGAAAG GAATCTGCTGGTTACAAGTGGGAAAGACACTATGGTAAAGTGGTGGGACCTGGATAACCAGCACTGCTTC AAAACAATGGTTGGCCACCGAACTGAGGTGTGGGGCTTGGTTCTGGTTTCAGAAGAAAAGCGACTAATCA CTGGGGCTGCAGACAGTGAGCTGAGAGCCTGGGACATCGACTATCTGCAAGAGATTGATGACCCAGAAGA ACCAGAGCCCAAGAAAATCAAAGAGTGTCCTGGAATACAGGACACACCTGAATCAGAGGACAGCACCCTT GAGGCGGATGATGAAAAATCTGAAGATCGCATACTTTCGTGTAGTAAAGCTGGCTCCATAATGCGGGAAG GAAGGGACAGAGTTGTGAACCTCGCCGTGGACAAGACAGGCAGGATTCTTGCTTGCCACGGAACTGATTC TGTGTTAGAAGTATTTTGTATTCTTTCCAAAGCAGAAGTTCAGAAGAAAATGGATAAGAAGCTAAAGAAA GCTAGAAAGAAAGCAAGGTTGAACTCTGCCAATGAGGAGGAGGACCCTGAAACGAGTGTCTCGATGACAC TGCAGGACGAGATCCTTCGAGTAGCCAAGATCAAGACTTCTGCCAAGATCAAATCTTTTGACCTGATTCA CTCGCCTCAAGGAGAGTTAAAGGCCGTCTTCTTGCTGCAGAACAATCTGGTGGAGTTGTATTCGCTCAAT GCGTCCTTGCCAGCCCCTCAGCCTGTGAGGACAAGCCGGATCACCATTGGAGGCCACCGCAGCGACGTGC GCACTTTGTCCTTCAGCTCGGATAATATAGCTGTGCTTTCAGCAGCAGCCGATTCCATTAAGATCTGGAA CAGGTCTACCCTGCAGTGTATCCGCACCATGCCTTGTGAATATGCTCTCTGCTCCTTCTTCGTTCCCGGC GATAGGCAGGTGGTCATAGGAACAAAGACAGGAAATCTGCAACTTTACGACCTGGCCTCAGGCACTCTCT TGGAGACAATAGCTGCCCATGATGGAGCCCTGTGGTCCATGTCTCTTTCTCCAGATCAGCGTGGCTTTGT GACAGGTGGCGCTGACAAAGCTGTCAAGTTCTGGGATTTTGAGTTGGTGACAGATAAAAATAGTACCCAG AAGAGACTTTCTGTGAAGCAGACTCGAACCTTGCAGCTAGATGAGGACGTCCTGTGTGTCAGCTACTCTC CCAATCAAAAGCTGCTGGCTGTGTCTTTGCTGGACTGCACTGTGAAGGTTTTCTATGTTGACACTTTAAA GTTTTTTCTCTCACTGTATGGACACAAACTGCCTGTTTTATGCATGGACATCTCCCATGATGGAGCACTA ATAGCAACTGGTTCTGCTGATAGAAATGTGAAAATCTGGGGTCTGGACTTTGGGGACTGCCACAGGTCTC TCTTTGCACATGATGACAGTGTGATGTATCTACGCTTTGTGCCTAAGTCTCACCTCTTCTTCACCGCTGG GAAGGACCACAAGATCAAGCAGTGGGATGCAGACAAGTTTGAGCACATCCAAACGCTGGAGGGACACCAC CAGGAAATATGGTGTTTGGCTGTCAGCCCCAGTGGAGACTACGTTGTATCAGCATCTCATGACAAGTCGC TGAGACTCTGGGAGAGAACGAGAGAGCCTCTTATTCTCGAGGAAGAAAGGGAAATGCAAAGGGAAGCAGA GTATGAGGAGAGCGTGGCCAAAGAAGACCAGCCGGCAGTTCCAGGGGAGACCCAAGGTGACAACTATTTC ACTGGGAAGAAGACAATTGAAACAGTCAAAGCGGCTGAGAGGATCATGGAGGCCATCGAGCTGCATCGAG AAGAAACTGCAAAAATGAAAGAACACAGAGCCATTTGTAAAGCAGCGGGGAAAGAGGTTCCTCTTCCTGT CAACCCCATCCTGATGGCTCATGGCAATATTTCTCCTTCAGCGTATGTGTTAGAGACTTTTAAAGGGATC AGGTCAAGTGAACTAGAAGAGGCCCTGCTTGTGCTGCCATTCTCCTATGTCCCAGACGTTCTTACACTGT TCAATGAATTCATCCAGACGGGCTTGGATGTCGAGCTCTTGTGCCGGTGCCTCTTTTTTCTCCTCAGGAT TCACTTTGGCCAGATCACCAGCAACCAGATGCTTGTGCCCGTGATAGAGAAACTAAAGGAAACAACTATT TCAAAAGTCAGACAAGTCCAGGATGCCATTGGCTTCAATATGGCTGGTCTCGATTATCTCAAGAGAGAGT GTGAAGCAAAGAGTGAAGTGATGTTTTTTGCAGAAGCTACTAGTCACTTGGAAGAGAAGAAGAAAAAACG AAAAAACAGGAAAAGGATGATTTTAACACTGACTTAGAACTGGAATGCAGTGTTTTTTCCTGCTTCTTCT CAAAGGAGCGTACACATAAACTGAGCTGTTGTGGAGTTGGCAACATAAAAATACCAAAAAAAGATCATGT TGTAGATCTTGGGGTACAGATGTCAGCCTTGGTTGAAGAAACCAACATGGCTGTGACTACTGTCTTTCAT CTTTTGAAAAGGACGGATTTAGTTTAAAAATCTGCATTTTTCAGAGTTGGACCTTTAAATTGTGTCAAGA AGCAAAATAATATTTTTTAATTGTGTATTTGATTCCGAAGTGTCTCATATTTATGGCAGATTATTTGTAT TAGAGCTTTTCTGTTTACCGTGTGTGTCATTGTCCTCTAAAGGAAGCGCCATGGACTGGGGCTGAAAGGG ATGATGGCCTGCTAAGAGCTGCCTGCTTACACCAGTGCAGGTTGGTGAGTCTGCATGTAAGGTCATAATG CTGTTTGTACTTTTGGAAATACAATAGCTTTTTCATTAAAACCACTTAAAATGAAAAAAAAAAAAACAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6332749 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6332749 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17429 same embryo
	EMAGE:17428 same embryo
	EMAGE:17433 same embryo
	EMAGE:17432 same embryo
	EMAGE:17431 same embryo
	EurExpress:euxassay_003872 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source