EMAGE mouse gene expression spatial database

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EMAGE:17432

Supv3l1 suppressor of var1, 3-like 1 (S. cerevisiae) ( MGI:2441711)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003869. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003869_01	euxassay_003869_02	euxassay_003869_03	euxassay_003869_04

euxassay_003869_05	euxassay_003869_06	euxassay_003869_07	euxassay_003869_08	euxassay_003869_09

euxassay_003869_10	euxassay_003869_11	euxassay_003869_12	euxassay_003869_13	euxassay_003869_14

euxassay_003869_15	euxassay_003869_16	euxassay_003869_17	euxassay_003869_18	euxassay_003869_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17432_wholemount_strong.wlz

17432_wholemount_moderate.wlz

17432_wholemount_weak.wlz

17432_wholemount_possible.wlz

17432_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17432_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7818
Entity Detected:	Supv3l1, suppressor of var1, 3-like 1 (S. cerevisiae) ( MGI:2441711)
Sequence:	sense strand is shown >T7818 CGGCCTCAATGTCCTTGCCTCGGTGTACCTTGCTGTGGGCTCGGCTCCCGGCGGGGCGCGGGGCTGGGCC CCGGGCGGCCCCTTGCTCCGCCCTTCGCGCTCTCGTGGGGTCGTTCCCGGGGGCTTCAGGACGCGTTCCC TGCTTGGCCGCCTCCTCCTCTGCCTCTGGTGGCTCCAAAGCCCCGAACACGTCCTTGTTCGTGCCGCTGA CGGTGAAGCCTCAGGGTCCCAGCGCCGATGGCGACGTGGGCGCGGAGCTCACTCGGCCTCTAGACAAGAA TGAAGTAAAGAAGATCTTAGACAAGTTTTATAAGAGGCAAGAAATTCAAAAGCTGAGTGCTGACTATGGA CTTGATGCTCGTCTCTTCCACCAGGCTTTCATAAGCTTCAGGAATTACATTATGCAATCCCATTCTCTGG ACGTGGACATTCACATTGTCCTGAATGATATCTGCTTCAGTGCAGCTCATGTGGACGATTTATTTCCATT TTTCTTGAGACATGCCAAACAAATATTTCCTGTATTGGAATGCAAGGATGATCTACGTAAAATCAGTGAC TTGAGGATACCACCCAACTGGTACCCAGAAGCCAGAGCGAGACAGCGGAAGATAATATTCCACTCAGGCC CTACCAACAGTGGGAAGACTTACCATGCCATCCAGAGATACCTGTCAGCGACCTCTGGCGTGTACTGCGG TCCTCTAAAACTGCTGGCGCACGAGATCTTTGAGAAGAGCAATGCTGCTGGTGTGCCGTGTGACTTGGTG ACAGGTGAAGAGCGGCTGACAGTGGAGCCTGAGGGGAAACAAGCCACCCATGTCTCTTGCACTGTGGAGA TGTGCAATGTTGCAACTCCTTATGAAGTGGCAGTGATAGATGAAATCCAGATGATTCGAGACCCAGCCAG AGGATGGGCTTGGACCAGAGCACTGCTAGGGCTCTGTGCCGAGGAAGTCCATTTATGTGGAGAGTCTGCT GCTATTAACCTGGTCTCTGAGCTCCTGTACACCACTGGGGAGGAAGTAGAGGTTCAGAAGTATGAGAGGC TTACCCCCATTTCTGTGCTGGATCATGCGCTGGAGTCTTTGGACAACCTTCAGCCTGGGGACTGCATTGT CTGCTTTAGCAAGAATGACATTTATTCTGTGAGCCGACAGATTGAAATTCGGGGACTGGAATCCGCGGTG ATATACGGCAGCTTGCCACCTGGGACCAAACTTGCTCAAGCAAGAAAGTTTAATGACCCCAATGATCCAT GCAAGATCCTGGTAGCCACAGATGCAATTGGCATGGGACTTAATTTGAGCATAAGGAGAATTATTTTTTA CTCCCTTATAAAGCCCAGCATCAATGAAAAGGGAGAGAAGGAGCTGGAGCCCATCACCACCTCCCAAGCC CTGCAGATTGCAGGCAGAGCTGGCAGATTCAGCTCACACTTTAAAGAGGGGCAGGTTACCACAATGCACC GAGACGACCTGGCTTTATTGAAGGACATTTTGAATAGACCTGTGGATCCTATACAGGCCGCCGGTCTCCA CCCGACTGCCGAGCAGATTGAGATGTTTGCCTACCACCTCCCCGAGACAACGCTGTCCAATCTCATTGAT ATTTTTGTAGACTTTGCACAAGTTGATGGGCAGTACTTTGTCTGCAATATGGATGATTTTAAGTTCTCTG CAGAGCTGATCCAGCATATCCCACTAAGTCTCCGGGTGAGGTATGTGTTCTGCACAGCCCCCATCAATAA GAAGCAGCCTTTTGTCTGCTCGTCGTTGTTACAGTTTGCCAGGCAGTACAGCAGGAATGAGCCCCTGACC TTTGCATGGCTACGCCGGTACATCAAGTGGCCTCTGCTTCCGCCTAAGAATATTAAAGACCTCATGGATC TTGAAGCTGTCCATGACGTCTTTGATCTTTACCTGTGGCTAAGCTACCGATTTATTGATATGTTTCCAGA CTCTAGCCTTGTTCGAAGTCTCCAGAAAGAGCTGGATGCCATTATCCAAGAAGGCGTGCACAACATCACC AAGCTGATTAAAATCTCTGAGTCACGCAAGCTTTTGAATCTGGAGAGCTTACCATCGGGGGACCAGTCAC GGTTGTCAGGAGCCTCCAAGAGCCCAGCGAGAAGGACACGTGGCACCAAAAGTGCAGGGAATAAGGCCAC AGAGCCACTCAGCCCCAGTGACAAGGAGCTGCCCCTTGCCTCTAGGTTGGTGCAGCAAGGACTCCTCACT GCAGACATGCTCAGGCAGCTCCAGAAGGAGTGGCTGACACAGCAGCCAGAACACAGCAGAGAGAAAGTGG GGACACGGAGAAAGAAGAAGGACCCCGACTCCGATTAGGTTTTTTTAATTTTGCAAATAAAAATCAATTT TAAATCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6332552 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6332552 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17429 same embryo
	EMAGE:17428 same embryo
	EMAGE:17433 same embryo
	EMAGE:17431 same embryo
	EMAGE:17430 same embryo
	EurExpress:euxassay_003869 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source