EMAGE mouse gene expression spatial database

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EMAGE:17501

Selm selenoprotein M ( MGI:2149786)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005831. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005831_01	euxassay_005831_02	euxassay_005831_03	euxassay_005831_04

euxassay_005831_05	euxassay_005831_06	euxassay_005831_07	euxassay_005831_08	euxassay_005831_09

euxassay_005831_10	euxassay_005831_11	euxassay_005831_12	euxassay_005831_13	euxassay_005831_14

euxassay_005831_15	euxassay_005831_16	euxassay_005831_17	euxassay_005831_18	euxassay_005831_19

euxassay_005831_20	euxassay_005831_21	euxassay_005831_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17501_wholemount_strong.wlz

17501_wholemount_moderate.wlz

17501_wholemount_weak.wlz

17501_wholemount_possible.wlz

17501_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17501_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 16 17 18 19 20 21

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 06 07 08 13 14

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 09 10

pons mantle layer

moderate

regional

moderate expression: see section 05 16

ventral grey horn

moderate

regional

moderate expression: see section 07 11 12 weak expression: see section 08 09 10

otic capsule

moderate

regional

moderate expression: see section 01 02 03 18 19 20 21 22

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 13 14 15 16 17 moderate expression: see section 07 18 weak expression: see section 06

cranium

moderate

regional

moderate expression: see section 01 02 03 04 19 20 21 22

orbito-sphenoid

moderate

regional

moderate expression: see section 05 06 07 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7014
Entity Detected:	Selm, selenoprotein M ( MGI:2149786)
Sequence:	sense strand is shown >T7014 CGGACGCGTCCGCCCACGCGTCCGTTGGAACCGTCTTCGAGGCCTGGCCAGGGGGCGGGTGGAGACCTGT GGAGGATGACAGTTGAATCGCCTAAAGGAGGTGAAGGCCTTTGTCACCGAGGACATTCAACTGTACCACA ACCTGGTGATGAAGCACCTCCCTGGGGCAGACCCCGAACTCGTGCTGTTAAGCCGAAATTACCAGGAACT AGAGCGAATCCCACTCAGCCAAATGACCCGGGACGAGATCAATGCGCTGGTACAGGAGCTCGGCTTCTAC CGCAAGTCGGCGCCGGAAGCTCAGGTGCCCCCCGAGTACCTGTGGGCGCCCGCTAAGCCCCCCGAGGAAG CTTCAGAACACGACGACCTGTAGCCCCGAGCCACAGTGGAGCTGGGACCCCCCCCTGCCAGCGCCCCGGA GACAGAATGAAGCGCTCAGTATCCCGGGAGCATCTCCCTTGCTGAGGGCCGACGCCAGTCTCCAAAGCAA CGGAAATGGGGTGTGTGGGGGGAATCTCAATCCTCCCTTTCCTCTCCCCAGCTCTACTAAATCTTCTTCC TTCAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3992699 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3992699 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17503 same embryo
	EMAGE:17504 same embryo
	EMAGE:17502 same embryo
	EMAGE:17505 same embryo
	EurExpress:euxassay_005831 same experiment
	MGI:4827943 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source