EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17502

Ptger3 prostaglandin E receptor 3 (subtype EP3) ( MGI:97795)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005830. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005830_01	euxassay_005830_02	euxassay_005830_03	euxassay_005830_04

euxassay_005830_05	euxassay_005830_06	euxassay_005830_07	euxassay_005830_08	euxassay_005830_09

euxassay_005830_10	euxassay_005830_11	euxassay_005830_12	euxassay_005830_13	euxassay_005830_14

euxassay_005830_15	euxassay_005830_16	euxassay_005830_17	euxassay_005830_18	euxassay_005830_19

euxassay_005830_20	euxassay_005830_21	euxassay_005830_22	euxassay_005830_23	euxassay_005830_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17502_wholemount_strong.wlz

17502_wholemount_moderate.wlz

17502_wholemount_weak.wlz

17502_wholemount_possible.wlz

17502_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17502_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7730
Entity Detected:	Ptger3, prostaglandin E receptor 3 (subtype EP3) ( MGI:97795)
Sequence:	sense strand is shown >T7730 CGCGGGAGGCGGCGGGCGATGGAGAGCAGAGCCTGGGCTCCGGCTGTCCCCCAGTGCACTCTGCTGCTAT CCCGCAGCTGAGCCGGGAGGCTCCGGCCCCGTGCGCCCTACCGTGGCCCCGCCACTATGGCTAGCATGTG GGCGCCGGAGCACTCTGCTGAAGCGCACAGCAACCTGTCAAGTACTACCGACGACTGCGGCTCCGTGTCC GTGGCCTTTCCCATCACCATGATGGTCACTGGCTTCGTGGGCAACGCGCTGGCCATGCTGCTCGTGTCGC GCAGCTACCGGCGCCGCGAGAGCAAGCGCAAGAAGTCTTTCCTGCTGTGCATTGGCTGGCTGGCGCTCAC CGACTTAGTGGGGCAGCTCCTGACCAGCCCGGTGGTCATCCTCGTGTACCTGTCACAGCGACGCTGGGAG CAGCTCGACCCATCGGGGCGTCTGTGCACCTTCTTCGGGCTAACCATGACAGTGTTCGGGCTATCCTCGC TCCTGGTGGCCAGCGCCATGGCCGTGGAGCGCGCCCTGGCCATCCGTGCGCCGCACTGGTATGCCAGCCA CATGAAGACTCGCGCCACGCGCGCGGTACTGCTGGGCGTGTGGCTGTCTGTGCTCGCCTTCGCGCTGCTG CCGGTGCTGGGCGTGGGCCGCTACAGCGTGCAGTGGCCGGGCACGTGGTGCTTCATCAGCACCGGGCCGG CGGGCAACGAGACAGACCCTGCGCGCGAGCCGGGCAGCGTGGCCTTTGCCTCCGCCTTCGCCTGCTTGGG CTTGCTGGCTCTGGTGGTGACCTTTGCCTGCAACCTGGCGACCATCAAAGCCCTGGTGTCCCGCTGTCGG GCCAAAGCCGCCGTCTCGCAGTCCAGCGCCCAGTGGGGCAGAATCACCACGGAGACGGCCATCCAGCTCA TGGGGATCATGTGTGTGCTGTCCGTCTGTTGGTCGCCGCTATTGATAATGATGTTGAAAATGATCTTCAA TCAGATGTCGGTTGAGCAATGCAAGACACAGATGGGAAAGGAGAAGGAGTGCAATTCCTTTCTAATTGCA GTTCGCCTGGCTTCGCTGAACCAGATCTTGGATCCCTGGGTTTATCTGCTGCTAAGAAAGATCCTTCTTC GGAAGTTCTGCCAGATGATGAACAACCTGAAGTGGACTTTCATTGCAGTACCTGTTTCCCTGGGTCTGAG AATTTCTTCTCCCAGGGAAGGATGACTGAGTATTTTGGATTGTATCTTCTTTTGGCCTCAATTTTAAGTT TTCCTTGCCATTAAACACACCGAGACAAGCTTTCTTAGGATAATCTGAGAGTCTGGTTGTTAGCTGGTTC CTGTGAAGGACTGAAGACTCTGCACTTGAGACGGGGGCAAGACGACACAGAGCAGCATGGAGAGACTCAG TGCAGAAATATCTCCAGCCTCAGAACCTTTGTGGACATGGACACCTTCATGTATTGATAGTCTGACTCTT CTAAATAGGTCTGAAAAAGCAGCATAAGTTTTTAAACAGTGAAGCATCAATGTGTTGAGAGCAAATGTTC ATCTAATAAGCCATGAGCCAAACAAGACAAAAAAGTCTACATGAGAGGGCAAGAGAGATTGTGCAAAGGG TATTTGTGCCAAGAAGGTATACAGTACCACAGAGTTGTGTCCTCAGTGAGAGTGGGAAATAAGTTTCTAA TTTAATTCTAATTACTGGCTCCTCAGTAATTCAGGAATCGTGCCATCATTTCCCTGCTTTTAAAGGGAGA AGTTTAGCTAAAGACACATTCCAGGTGTCACTAACAGTTCCAAAGCTAGGTGACTAAATGTTCAGCTAGA GCTGTTAAAAGGAAAACCAGCTAATTATCATTCCAGTCCAATGCTATTTTTGAATTACTATCTACTTAAG ATTTCTCATAATTTGTGCTCAGGCAGCACAATAAAAAGGGGGGGGCAAAATTACTAAGTGACAGTTATTC TGCATCTAAGTCTGTGACTTTTTTATGAAATAAAATGATTTTGTCTGTGTTGAAATAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6309451 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6309451 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17503 same embryo
	EMAGE:17504 same embryo
	EMAGE:17501 same embryo
	EMAGE:17505 same embryo
	EurExpress:euxassay_005830 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source