EMAGE mouse gene expression spatial database

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EMAGE:17505

Fank1 fibronectin type 3 and ankyrin repeat domains 1 ( MGI:1914180)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005846_01	euxassay_005846_02	euxassay_005846_03	euxassay_005846_04	euxassay_005846_05

euxassay_005846_06	euxassay_005846_07	euxassay_005846_08	euxassay_005846_09	euxassay_005846_10

euxassay_005846_11	euxassay_005846_12	euxassay_005846_13	euxassay_005846_14	euxassay_005846_15

euxassay_005846_16	euxassay_005846_17	euxassay_005846_18	euxassay_005846_19	euxassay_005846_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17505_wholemount_strong.wlz

17505_wholemount_moderate.wlz

17505_wholemount_weak.wlz

17505_wholemount_possible.wlz

17505_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17505_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7748
Entity Detected:	Fank1, fibronectin type 3 and ankyrin repeat domains 1 ( MGI:1914180)
Sequence:	sense strand is shown >T7748 CGAAGACAGGCGTTAGGAGGGCGGCGCCGCCAGGGCTGGGCCCGAGGGCCCCCGGGAGCAGCTGACCATG GAGCCCCACAAAGTCGTGCCACTCTCAAAGCCGCATCCTCCTGTTGTGGGCAAGGTGACTCATCACAGCA TCGAATTGTACTGGGATCTGGAACAGAAAGAGAAACGGCAAGGACCACAGGAGCAGTGGCTTCGGTTCTC CATTGAGGAGGAGGACCCCAAGATGCACAGCTACGGTGTCATTTACACGGGTTATGCAACAAGGCATGTG GTTGAAGGCCTAGAACCAAGGACACTATACAAATTTCGACTGAAGGTCACCAGCCCCTCTGGAGAATATG AGTACAGCCCAGTCGTCTCGGTGGCTACGACCCGGGAGCCCATAAGTAGCGAACACTTTCATCGTGCTGT CAGTGTGAACGATGAAGATCTCCTGCTTCGAATTTTGGAAGGAGGCCATGTCATGATTGACGTGCCCAAC AAATTTGGCTTTACCGCTCTCATGGTTGCTGCTCAGAAAGGTTACACCAGGCTTGTGAAGATCCTAGTTT CCAATGGCACAGATGTGAATCTGAAGAACGGAAGTGGCAAGGACAGTCTCATGCTTGCATGCTACGCGGG GCACCTAGATGTTGTCAAATACCTCCGGAGACACGGAGCTTCTTGGGAGGCTCGAGACCTCGGAGGCTGC ACAGCTCTGCACTGGGCTGCAGATGGAGGCCATTGCTCTGTGATTGACTGGATGATAAAGGACGGTTGTG AGGTAGATGTTGTAGACACTGGTTCAGGATGGACGCCACTCATGAGAGTCTCCGCTGTGACAGGAAGCCA GAAGGTGGCCTCACTCCTCATCGAGGCTGGGGCTGACGTGAACATCAAGGACAAAGATGGAAAGACGCCT CTCATGGTCGCTGTCTTAAATAATCACGAACAGCTAGTTCAGCTACTTCTTGATAAAGGGGCAGATGCAA CTGTGAAAAATGAGTTTGGCAAAGGTGTCCTAGAAATGGCCAGAGTTTTTGACAGACAGAATGTACTTTC CTTACTGGAAGAAAAGAAAAAAAAGATGCCAAGGAAGTCCTCTGTCCACTGAGCAGAGTGCTACTCATCT GAGAGACCGACTTGAGAGAACAGCAAACTGTGACCACCGGGCTGAACACGTGACGTGCAGCATCTCTGAG GACGAGGTGTTTATTTATTTAGTTGAAGATTCACAGTGACTTTTATGTAATGGGCAACTCTTCCCACATT GAAATACATCTTTTTACCTAAGCATGTTCATATGTGGTCTCATCACTGGGCCTCCGTAGAACAGGAATGT TTTCTTGCCCTGTGCCTTTTGCAGTTAGACACAAAACCAAATACTTACGCATTGGTGTTACATAAACGAG AGTGCATGGGTCCCCACCCCTGTCTCCCCTTCCCCTTAGCTGTCCTCGAGATGCTCCAGGCCTCCTGGCA CCTTCCACTCATGTGTTTCTTATTATCTCTCCCACCCCACTGCAGGCCAGGCTGCTGTTGCCTCCTGCGG CGGGCGGGGCTCCTCCTAAGCACCCAGGAGTTCCCTCTTAGGCTCTTGGCCTCACATCAAAGCCTAGAAT ACATATGTGTAGGCTGGCGTGGTTCTGTTTGCAGTGTTTAGTTGAAGGACAGGGCTGCGAGGGCAGGTCC ATCGGTTCATGTGTCTGCCCATGCTCACCTCTGTGGTGAAAGGACCCAGGCCCTGGACAGCCTCCCCAGT GATTAGTGTGTTACATTGAGAGTAGATACGATTTTTGAGGCAAATCTTATAGGTTGTAGACAAAAAGGGG TTAAGACACCAAGACTGTCAGTTAAGAGCCCAAAGATAGAAATGTGGGAAGTTTTAGCTGGACCAAGCTT AAACATCAGCAGAAACATGTGCATCTTGTGTTTCCAGGGACAAACTGGTTCATTTAGCCTTCTAATAAAG CAGCTAGAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6310911 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6310911 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17503 same embryo
	EMAGE:17504 same embryo
	EMAGE:17502 same embryo
	EMAGE:17501 same embryo
	EurExpress:euxassay_005846 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source