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EMAGE:18217

Cdkn1b cyclin-dependent kinase inhibitor 1B ( MGI:104565)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18217 EMAGE:18217 EMAGE:18217 EMAGE:18217 EMAGE:18217
"Pseudo-wholemount" of euxassay_011991. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011991_01 euxassay_011991_02 euxassay_011991_03 euxassay_011991_04
EMAGE:18217 EMAGE:18217 EMAGE:18217 EMAGE:18217 EMAGE:18217
euxassay_011991_05 euxassay_011991_06 euxassay_011991_07 euxassay_011991_08 euxassay_011991_09
EMAGE:18217 EMAGE:18217 EMAGE:18217 EMAGE:18217 EMAGE:18217
euxassay_011991_10 euxassay_011991_11 euxassay_011991_12 euxassay_011991_13 euxassay_011991_14
EMAGE:18217 EMAGE:18217 EMAGE:18217 EMAGE:18217 EMAGE:18217
euxassay_011991_15 euxassay_011991_16 euxassay_011991_17 euxassay_011991_18 euxassay_011991_19
EMAGE:18217 EMAGE:18217 EMAGE:18217 EMAGE:18217 EMAGE:18217
euxassay_011991_20 euxassay_011991_21 euxassay_011991_22 euxassay_011991_23 euxassay_011991_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18217Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18217_wholemount_strong.wlz
18217_wholemount_moderate.wlz
18217_wholemount_weak.wlz
18217_wholemount_possible.wlz
18217_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18217_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
spleen primordium
strong strong
regionalstrong expression: see section 06 07
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30080
Entity Detected:Cdkn1b, cyclin-dependent kinase inhibitor 1B ( MGI:104565)
Sequence:sense strand is shown

>T30080
TCGTGCTTTTGGGGTGTTTTTCCCCCCTCATCCCTTGTCCCGACTCACTCGCGGCTCCGAGACTCGCGCG
GCGGCAAGGTTTGGAGAGGGGCTGCGTTCGCGGGACACACGGGCTCGCCCCAGCCTACGCTCCGACTGTT
TGCCACCTCCTCCTGCCTCCTCCCCTCCCTTCCCCGCCCTCCAGTACACTTGATCACTGAAGCCTCGAGC
TGCGCGGCGGCTGGGGTGTCCCTGCGCCTCTCTTCCCCAGACCTGCGCGCTACTGCGGCTCGGGCGGTCG
CTCGCCTGGCTCTGCTCCATTTGACTGTCTGTGTGCAGTCGCAGAACTTCGAAGAGGGTTTTGCGCTCCA
TCCGTGGCGTTTCGCTTTTGTTCGGTTTTGTTGTTTATTTCATTTTTTTTTTTCCGGAGAGAGGCGAGGC
GGTGGTCCACACCCGCCCGAGGAGGAAGATGTCAAACGTGAGAGTGTCTAACGGGAGCCCGAGCCTGGAG
CGGATGGACGCCAGACAAGCGGATCACCCCAAGCCTTCCGCCTGCAGAAATCTCTTCGGCCCGGTCAATC
ATGAAGAACTAACCCGGGACTTGGAGAAGCACTGCCGGGATATGGAAGAAGCGAGTCAGCGCAAGTGGAA
TTTCGACTTTCAGAATCATAAGCCCCTGGAGGGCAGATACGAATGGCAGGAGGTGGAGAGGGGCAGCTTG
CCCGAGTTCTACTACAGGCCCCCGCGCCCCCCCAAGAGCGCCTGCAAGGTGCTGGCGCAGGAGAGCCAGG
ATGTCAGCGGGAGCCGCCAGGCGGTGCCTTTAATTGGGTCTCAGGCAAACTCTGAGGACCGGCATTTGGT
GGACCAAATGCCTGACTCGTCAGACAATCAGGCTGGGTTAGCGGAGCAGTGTCCAGGGATGAGGAAGCGA
C
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4193493), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17385. Forward Primer - name:017385_F_IRAV20-23_H04_Cdkn1b, sequence:TCGTGCTTTTGGGGTGTT; Reverse Primer - name:017385_R_SP6_IRAV20-23_H04_Cdkn1b, sequence:GGTCGCTTCCTCATCCCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18218 same embryo
 EMAGE:18220 same embryo
 EMAGE:18215 same embryo
 EMAGE:18219 same embryo
 EMAGE:18216 same embryo
 EurExpress:euxassay_011991 same experiment
 MGI:4823785 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS