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EMAGE:18219

Vdr vitamin D receptor ( MGI:103076)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18219 EMAGE:18219 EMAGE:18219 EMAGE:18219 EMAGE:18219
"Pseudo-wholemount" of euxassay_012002. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012002_01 euxassay_012002_02 euxassay_012002_03 euxassay_012002_04
EMAGE:18219 EMAGE:18219 EMAGE:18219 EMAGE:18219 EMAGE:18219
euxassay_012002_05 euxassay_012002_06 euxassay_012002_07 euxassay_012002_08 euxassay_012002_09
EMAGE:18219 EMAGE:18219 EMAGE:18219 EMAGE:18219 EMAGE:18219
euxassay_012002_10 euxassay_012002_11 euxassay_012002_12 euxassay_012002_13 euxassay_012002_14
EMAGE:18219 EMAGE:18219 EMAGE:18219 EMAGE:18219 EMAGE:18219
euxassay_012002_15 euxassay_012002_16 euxassay_012002_17 euxassay_012002_18 euxassay_012002_19
EMAGE:18219 EMAGE:18219 EMAGE:18219 EMAGE:18219
euxassay_012002_20 euxassay_012002_21 euxassay_012002_22 euxassay_012002_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18219Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18219_wholemount_strong.wlz
18219_wholemount_moderate.wlz
18219_wholemount_weak.wlz
18219_wholemount_possible.wlz
18219_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18219_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30083
Entity Detected:Vdr, vitamin D receptor ( MGI:103076)
Sequence:sense strand is shown

>T30083
CATTGTCTCCCCAGACCGACCTGGGGTACAGGATGCTAAGCTGGTTGAAGCCATTCAGGACCGCCTATCC
AACACACTGCAGACCTACATCCGCTGCCGCCACCCGCCCCCGGGCAGCCACCAGCTCTACGCCAAGATGA
TCCAGAAGCTGGCTGACCTGCGAAGCCTCAATGAGGAGCACTCCAAACAGTACCGTTCCCTCTCCTTCCA
GCCGGAGAACAGCATGAAGCTCACACCCCTTGTGCTAGAGGTGTTCGGCAATGAGATCTCCTGACCAGGG
TGGCCTGCAGTGGTGCCTGGGTAGGGCCGCTCCTCTGGGGCCCTGTGCCCAGGCCCTGGGCTGGTTGCAG
CCCAGCAGTGCCTCCTGCCCCTCCTGGAGTTCAGCTCCTCCTCTGCCGTGGCCCGTGTCTGTCTGGCTCA
TCCTTTCTCCTGCCCAGCCTCACACCTGATCTCCCTTTCCTGTAGACTGCAGGTTGCTCCTGTCCCTTGA
GATCTCAGTTAGGAGACACTGCTGTTTATTTGACAAAGAAACTCAAGTGTGGGATGGGGGGAAGAGGGTA
GAGGGCAGAGGCTGAAGGCAGAGAGCTCTGCCTAGGGAATGCCTCCGCCATAAGGGGCCACTGCTTGTGT
CAAGGGAGGCAGGCAGAAGAGATGAGTCCATTCCTCAGGGACAGGTACCTGTACCTACCCCGATTCCAAA
CCTACCTGCCTGGTGAGAACTCCTGCCCCTGCCTACAAAGGGTACACAACCTACCCATCATCCCTAGTGT
GTCCCGTCTCCTCCTGCCACCTGTCTGTATTATTCTGACCCAGGGGAGTCAGTCACTGTGGGGCCTCCTT
CCTCTGCTGGTATACTCATGGACTCATTCACTGCCAAGATGACCAAATACTCTACCACACTAACCAAGGA
GCCCTCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3710866), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 16550. Forward Primer - name:016550_F_IRAV20-23_I02_Vdr, sequence:CATTGTCTCCCCAGACCG; Reverse Primer - name:016550_R_SP6_IRAV20-23_I02_Vdr, sequence:GGGAGGGCTCCTTGGTTA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18217 same embryo
 EMAGE:18218 same embryo
 EMAGE:18220 same embryo
 EMAGE:18215 same embryo
 EMAGE:18216 same embryo
 EurExpress:euxassay_012002 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS