EMAGE mouse gene expression spatial database

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EMAGE:18247

Deaf1 deformed epidermal autoregulatory factor 1 (Drosophila) ( MGI:1858496)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012020. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012020_01	euxassay_012020_02	euxassay_012020_03	euxassay_012020_04

euxassay_012020_05	euxassay_012020_06	euxassay_012020_07	euxassay_012020_08	euxassay_012020_09

euxassay_012020_10	euxassay_012020_11	euxassay_012020_12	euxassay_012020_13	euxassay_012020_14

euxassay_012020_15	euxassay_012020_16	euxassay_012020_17	euxassay_012020_18	euxassay_012020_19

euxassay_012020_20	euxassay_012020_21	euxassay_012020_22	euxassay_012020_23	euxassay_012020_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18247_wholemount_strong.wlz

18247_wholemount_moderate.wlz

18247_wholemount_weak.wlz

18247_wholemount_possible.wlz

18247_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18247_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 05 06 07 17 18 weak expression: see section 19 20 21 22 23 24

radius

strong

regional

strong expression: see section 01 02

ulna

strong

regional

strong expression: see section 01 02

humerus

strong

regional

strong expression: see section 01 02 03 05 23 24

hip

strong

regional

strong expression: see section 12 13 19 20

fibula

strong

regional

strong expression: see section 05 06 08 moderate expression: see section 07

tibia

strong

regional

strong expression: see section 05 06 08 09 10 moderate expression: see section 07

femur

strong

regional

strong expression: see section 05 06 08 09 10 22 23 24 moderate expression: see section 07

otic capsule

moderate

regional

moderate expression: see section 17 18 weak expression: see section 07 08 09 10 19 20 21

nasal septum

strong

regional

strong expression: see section 15 16 17 18 19 20

meckel's cartilage

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

axial skeleton

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 moderate expression: see section 17

basioccipital bone

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17

basisphenoid bone

strong

regional

strong expression: see section 11 12 13 14 15 16 17

exoccipital bone

strong

regional

strong expression: see section 19 20 21 22 23 24

temporal bone

strong

regional

strong expression: see section 20 21 22 23 24

orbito-sphenoid

strong

regional

strong expression: see section 07 08 09 10 12 13 14 15 16 20 21 22 23 24

viscerocranium

strong

regional

Expression in the turbinate bone.

clavicle

moderate

regional

moderate expression: see section 10 21 weak expression: see section 18 19 20

scapula

strong

regional

strong expression: see section 01 02 03 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2840
Entity Detected:	Deaf1, deformed epidermal autoregulatory factor 1 (Drosophila) ( MGI:1858496)
Sequence:	sense strand is shown >T2840 TCNAGCCAGATTCGGCACGAGGCCCAGGCGAGAATGCAGGTTGATACAGAGAGGAAAGAGCAGTCATGTG TCAACTGCGGCCGGGAGGCCATGAGTGAGTGTACCGGCTGCCACAAGGTTAACTACTGCTCTACGTTCTG CCAGCGCAAGGACTGGAAAGACCATCAGCATGTGTGTGGCCAGTCAGCGTCTGTCACTGTCCAGGCTGAT GACGTCCATGTTGAAGAAAGTGTGATAGAAAAAGTTGCTGTTTGAGCCAGGCTTCTCCTTGAGCTCTGCT GTGAAGCCATCGCAGGCTTAGGCCCTCTGATGACTGTGGGGGCTGCTGAGAAGAGGGGCCATGGGAAACT TGCCGGACAAGTTATTAACAGATTGTGCCCTTGGAAACAAGCTCCTTCCAGAGACCAAGGAACGCTGGCA GCTTTCAGAATGGGGCACTCTGCTACAGCCCAGGGCCCTCAGCCTATCACGGTGCTCTTTCATCCCTGGA TCATGAGTGTCCCACTACCCTTGGGGTGCCCTATGCCACGTGTACATACCTGTCTTATCTGTTAATAAAC ATGTAAA
Notes:	The probe template was PCR amplified from IMAGE:1528917 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1528917 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18246 same embryo
	EMAGE:18245 same embryo
	EMAGE:18248 same embryo
	EMAGE:18250 same embryo
	EMAGE:18249 same embryo
	EurExpress:euxassay_012020 same experiment
	MGI:4824275 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source