EMAGE mouse gene expression spatial database

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EMAGE:18250

Ifi205 interferon activated gene 205 ( MGI:101847)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012034. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012034_01	euxassay_012034_02	euxassay_012034_03	euxassay_012034_04

euxassay_012034_05	euxassay_012034_06	euxassay_012034_07	euxassay_012034_08	euxassay_012034_09

euxassay_012034_10	euxassay_012034_11	euxassay_012034_12	euxassay_012034_13	euxassay_012034_14

euxassay_012034_15	euxassay_012034_16	euxassay_012034_17	euxassay_012034_18	euxassay_012034_19

euxassay_012034_20	euxassay_012034_21	euxassay_012034_22	euxassay_012034_23	euxassay_012034_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18250_wholemount_strong.wlz

18250_wholemount_moderate.wlz

18250_wholemount_weak.wlz

18250_wholemount_possible.wlz

18250_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18250_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 weak expression: see section 04 05 06 07 08

radius

strong

regional

strong expression: see section 01 02 03

ulna

strong

regional

strong expression: see section 01 02

humerus

strong

regional

strong expression: see section 01 02 03 04 05 weak expression: see section 23 24

hip

weak

regional

weak expression: see section 12 13 19 20

fibula

strong

regional

strong expression: see section 04 05 06 moderate expression: see section 07 08

tibia

strong

regional

strong expression: see section 04 05 06 moderate expression: see section 02 03 07 08 09

femur

strong

regional

strong expression: see section 04 05 06 moderate expression: see section 07 08 09 weak expression: see section 22 24

otic capsule

weak

regional

weak expression: see section 07 08

nasal septum

weak

regional

weak expression: see section 14 15 16 17 18 19

meckel's cartilage

strong

regional

strong expression: see section 03 04 05 06 07 08 moderate expression: see section 16 17 18 19 20 21 weak expression: see section 09 10 11 12 13 22 23 24

axial skeleton

moderate

regional

moderate expression: see section 07 weak expression: see section 08

exoccipital bone

moderate

regional

moderate expression: see section 01 02 03 04 05 23 24 weak expression: see section 20 21 22

temporal bone

moderate

regional

moderate expression: see section 01 02 03 04 05 06 23 24 weak expression: see section 20 21 22

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 08 09 23 weak expression: see section 20 21 22

viscerocranium

moderate

regional

Expression in the turbinate bone.

scapula

strong

regional

strong expression: see section 01 02 03 04 weak expression: see section 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8368
Entity Detected:	Ifi205, interferon activated gene 205 ( MGI:101847)
Sequence:	sense strand is shown >T8368 GGTGTCTTCATTTATACCCCATTTTTCACTTTCTCAGTTACTGAATTATCTGCCTACCTACTCAAACCAA GCAGGCCACTTCTGTTGTTGAAGATCTCAGCACCTGTACATTGCTGCCGAAATTCCAGGGAGTATAACCA ACAACTTGAAAGATGGAGAATGAATATAAGAGACTTGTTCTGCTGGAAGGACTTGAATGTATCAATAAGC ATCAATTCAATTTATTTAAGTCATTGATGGTCAAAGATTTAAATCTGGAAGAAGACAACCAAGAGAAATA TACCACGTTTCAGATTGCTAACATGATGGTAAAGAAATTTCCAGCTGATGCTGGATTGGACAGACTGATC AACTTTTGTGAACGTGTACCAACTCTTAAAAAACGTGCTGAAATTCTTAAAAAAGAGAGATCAGAAGTAA CAGAAGAAACATCACTGGAAATAAATAGGCAAGAAGCAAGTCCTGCAACACCTACATCAACTACAAGCCA CATGTTAGCATCTGAAAGAGGCAAGACTTCCACAACCCAGGAAGAGACTTCCACAGCCCAGAAAAGGAAA GGTATGAGTGAAGAAAAGACTGACGTGAAGAAGATCAAGGCATCTGGGAAAGCAGATCAGCCTCCCTGTT GTGAAGGACCCACAGCCACATGCCAGTCACCAATATCCCAGGTCTCATCTTCGGCTTCATCTAACATTCC TTCGGCTAAGAACCAAAAATCACAACCCCAGAACCAGAACATTCCCAGAGGTGCTGTTCTCCACTCAGAG CCCCTGACAGTGATGGTGCTCACTGCAACAGACCCGTTTGAATATGAATCACCAGAACATGAAGTAAAGA ACATGTTTCATGCTACAGTGGCTACAGTGAGCCAGTATTTCCATGTGAAAGTTTTCAACATCGATTTGAA AGAGAAGTTCACAAAAAATAATTTTATCACCATATCCAATTACTTTGAGAGCAAAGGCATCCTGGAGATC AATGAGACTTCCTCTGTGTTAGAGGCTGCTCCTAAACAAATGATTGAAGTGCCCAACTGTATTACCAGAA ATGCAAATGCCAGTCCTAAGATCTGTGATATTCAAAAGGGTACTTCTGGAACAGTGTTCTATGGAGTGTT TACATTACACAAGAAAAAAGTGAAAACACAGAACACAAGCTATGAAATAAAAGATGGTTCAGGAAGCATA GAAGTGGTGGGGAGTGGACAATGGCACAACATCAACTGTAAGGAAGGAGATAAGCTCCACCTCTTCTGCT TTCACCTGAAAAGAGAAAGAGGACAACCAAAGTTAGTGTGTGGAGACCACAGTTTCGTCAAGGTCACCAA GGCTGGGAAAAAAAAAGAAGCATCAACTGTCCAGTGAAGCACAAAAAATGAAGAAGAAAATGATTACCCA AAAGTTGGAATTAAGGTAGAGATGCCAAAATAGAAATGTCACCTCTAAATGACAGCTTTAGTAGTATATC CAACCATTGATTAATCTTCATACCTGATTTCTGATTTTGTGTTTTCATTTGAAAAAAATTCTTATTGATC TGTTTTTCTATGAAAATAAAATTTGATTTCATTTCTCTACTGTAAAAATAATAAACATGTCTTTTTAAAG GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3466347 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3466347 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18247 same embryo
	EMAGE:18246 same embryo
	EMAGE:18245 same embryo
	EMAGE:18248 same embryo
	EMAGE:18249 same embryo
	EurExpress:euxassay_012034 same experiment
	MGI:4825510 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source