EMAGE mouse gene expression spatial database

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EMAGE:18248

BC056474 cDNA sequence BC056474 ( MGI:3041257)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012032. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012032_01	euxassay_012032_02	euxassay_012032_03	euxassay_012032_04

euxassay_012032_05	euxassay_012032_06	euxassay_012032_07	euxassay_012032_08	euxassay_012032_09

euxassay_012032_10	euxassay_012032_11	euxassay_012032_12	euxassay_012032_13	euxassay_012032_14

euxassay_012032_15	euxassay_012032_16	euxassay_012032_17	euxassay_012032_18	euxassay_012032_19

euxassay_012032_20	euxassay_012032_21	euxassay_012032_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18248_wholemount_strong.wlz

18248_wholemount_moderate.wlz

18248_wholemount_weak.wlz

18248_wholemount_possible.wlz

18248_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18248_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10066
Entity Detected:	BC056474, cDNA sequence BC056474 ( MGI:3041257)
Sequence:	sense strand is shown >T10066 GAAGTTGCTCTTCTAGCTGTGAAAGAAGGACGGCGTGGTCTTCAGTTTGCTATGTCCACTAACAATATGT CCGACCCACGAAGGCCCAACAAAGTGCTGAGGTATAAGCCTCCCCCGAGTGAGTGCAACCCGGCTTTAGA CGACCCGACTCCGGACTACATGAATCTTCTTGGCATGATCTTCAGCATGTGTGGCCTCATGCTTAAGCTG AAGTGGTGTGCTTGGGTTGCTGTCTACTGCTCCTTTATCAGCTTTGCCAACTCGCGGAGCTCGGAGGACA CTAAGCAGATGATGAGTAGCTTCATGCTCTCTATCTCCGCCGTGGTGATGTCCTATCTGCAGAATCCTCA GCCCATGACGCCTCCCTGGTGATGCCACCTAGGAGGGTTGGATCCTGGACTCAGGCCCACCTTCTCTCTG GCCTAGCCTTTGGCTGCCTCCGCCCTCCCTCAGCTGCTGTCCTAAACTTTCCTGAGTGTGGTCTCTGGGC TCCCAGCTGAATGGAAGGAAGTTGGCTCTTTCTTTGGGGCCCTGCTTCTGCTTTGACAAAGAGATAAACC TGCAGACTTCCCTTTTACCTTCTCCCCCCCCCCCAAACCCGGCCACTGTTATGAGAAACACTGTTCATGT TTCTACGTAGTCACTTGTTTTCTTATTGAAACCAGTTGATATAAAACTTTCCGCTCTGGTTGGCTTGCAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30118707 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30118707 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18247 same embryo
	EMAGE:18246 same embryo
	EMAGE:18245 same embryo
	EMAGE:18250 same embryo
	EMAGE:18249 same embryo
	EurExpress:euxassay_012032 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source