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EMAGE:19337

Olfr568 olfactory receptor 568 ( MGI:3030402)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19337 EMAGE:19337 EMAGE:19337 EMAGE:19337 EMAGE:19337
"Pseudo-wholemount" of euxassay_002104. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002104_01 euxassay_002104_02 euxassay_002104_03 euxassay_002104_04
EMAGE:19337 EMAGE:19337 EMAGE:19337 EMAGE:19337 EMAGE:19337
euxassay_002104_05 euxassay_002104_06 euxassay_002104_07 euxassay_002104_08 euxassay_002104_09
EMAGE:19337 EMAGE:19337 EMAGE:19337 EMAGE:19337 EMAGE:19337
euxassay_002104_10 euxassay_002104_11 euxassay_002104_12 euxassay_002104_13 euxassay_002104_14
EMAGE:19337 EMAGE:19337 EMAGE:19337 EMAGE:19337
euxassay_002104_15 euxassay_002104_16 euxassay_002104_17 euxassay_002104_18

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19337Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19337_wholemount_strong.wlz
19337_wholemount_moderate.wlz
19337_wholemount_weak.wlz
19337_wholemount_possible.wlz
19337_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19337_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
strong strong
single cellstrong expression: see section 07 08 09 10 12 13 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5330
Entity Detected:Olfr568, olfactory receptor 568 ( MGI:3030402)
Sequence:sense strand is shown

>T5330
ACAACCATTGGCGTCTCTCGTGCAGGTTGAGCTCGAGAGATCATTTAATGCGTGTATTGCCCACTATGTT
CTTTCTCCATGGGTTTACTTTCATGGAATCCGGAGTTCTGCTGGCCATGGCCTTTGATCGTTTTGTGGCA
ATCTGTGATCCACTAAGATATACCACTATTCTTACCAATGCCAGGATTGCCCAGATTGGCACAATTGTGT
TGATAAGGAATGTTGCCGTCATGTTGCCAGTTGTGCTCTTTGTCAAGAGGTTGTCCTTCTGCAGTTCTCT
GGTTCTTTCACATTCATACTGCTACCATGTAGATGTCATCCAGCTCTCATGTACAGACAACAAAATCAAT
AGCGTTCTTGGTCTGTTTGCACTATTCTCTACTACAGGGTTTGACTGCCCTTGCATCTTGCTGTCCTATG
TACTGATCATCCGATCTGTCCTCAGCATTGCTTCCTCTGATGAGCGACAGAAAGCCTTCAATACATGCAT
ATCCCACATCAGTGCTGTTGCCATCTTCTACATTCCTCTCATCAGCTTGTCTCTTGTACACCGCTACGGC
CATTCAGCACCCGCCTTTGTCCACACCGTCATGGCCAACGTCTTCCTGCTCATCCCTCCTGTGCTCAACC
CTATAATCTACAGTGTGAAGACGAAGCAGATTCGAAAGGGCTATTCTCAAGGTGTTAAATCAGAAACAAA
ACCAGCTTTAACTATAGTGTACTATAATACACAGCATTGCACATTCTTCGGCCTCCCAGTTTGATAAAAA
AGGGTTTGACGTATGGAAGAAGGGCCACGGAAAACCTTGGCGTGGTTGGGACACAGGGGGGTGTCAAGGG
CAGGATACAAAAAACAACTGAAGAACAGGAAGGTTTAAAAATGGCCCCACCCTCATGGGGGGAAAAATGC
AAGGTTTTAGAAA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CTTGTCTCTCTGCACACTTTC; Reverse Primer - name:unspecified, sequence:TTAAAGCTGGTTTTGTTTCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19340 same embryo
 EMAGE:19339 same embryo
 EMAGE:19338 same embryo
 EMAGE:19341 same embryo
 EurExpress:euxassay_002104 same experiment
 MGI:4826925 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS