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EMAGE:19339

Olfr640 olfactory receptor 640 ( MGI:3030474)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19339 EMAGE:19339 EMAGE:19339 EMAGE:19339 EMAGE:19339
"Pseudo-wholemount" of euxassay_002105. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002105_01 euxassay_002105_02 euxassay_002105_03 euxassay_002105_04
EMAGE:19339 EMAGE:19339 EMAGE:19339 EMAGE:19339 EMAGE:19339
euxassay_002105_05 euxassay_002105_06 euxassay_002105_07 euxassay_002105_08 euxassay_002105_09
EMAGE:19339 EMAGE:19339 EMAGE:19339 EMAGE:19339 EMAGE:19339
euxassay_002105_10 euxassay_002105_11 euxassay_002105_12 euxassay_002105_13 euxassay_002105_14
EMAGE:19339 EMAGE:19339 EMAGE:19339 EMAGE:19339
euxassay_002105_15 euxassay_002105_16 euxassay_002105_17 euxassay_002105_18

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19339Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19339_wholemount_strong.wlz
19339_wholemount_moderate.wlz
19339_wholemount_weak.wlz
19339_wholemount_possible.wlz
19339_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19339_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
moderate moderate
single cellmoderate expression: see section 09 10 12 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5328
Entity Detected:Olfr640, olfactory receptor 640 ( MGI:3030474)
Sequence:sense strand is shown

>T5328
TCCTGTGAGGCCCTCGCTCCTCGTTGTATCGCTTCCTGGCATGACACGGCCGGCTTGGATTGCCCTGATT
TTCTGTTTCCTCTATTTCGTCTCCTTTGCAGGTTACCTCAGTATCCTTGCTATGGACATTCGAAAACCTC
CTCTACACCAACCCATGTACTATTTCCTCTCTATGCTCTCTCTCAATGACTTGGGAGTGTCCCTATCTAC
ACTTCCTACCGTACTTGCTACCTTCTGTTTCAATTACCGCCATGTTGATTTTGATGCCTGTCTGGTACAG
ATGTTCTTCATTCACACGTTTTCTTTCATGGAATCGGGCATACTACTTGCCATGAGCTTTGATCGTTTTG
TGGCTATCTGTGACCCACTACGCTATTCCACTGTGCTTACCAATAGCCGCATCCTGGCTATGGGTCTGGG
TATCCTTGCAAAAAGTTTCACTACCCTCTTTCCTTTCCCCTTTCTTGTAAAAAGATTGCCCTTTTGCAAG
GGCAATGTGCTACACCATTCATATTGCCTCCATCCAGATCTGATGAAAGTGGCATGTGGAGACATCCATG
TTAACAATATCTATGGGCTCTTTGTGGTAATTTACACATATGGTGTGGACTCAGTTTTTATCCCTTCTGT
CTTATGCACTGATCTTGAGAGCTGTGTTGGTTATTGCATCACACGAACAGCGACTCAAGGCACTCAACAC
CTGTATATCACACATCTGTGCAGTACTGGCATTTTATGTTCCCATAATTGCTTGTCCTCCATGATCCACC
CGCTTCTGGAAAAGTGCTCCAGCCTGTTTGTTCATGTTTATGATGTACAATGTCTATCTTATTCGTACCT
CCCATGCCTCCACCCCCATCATCTATAGCGTGAAGACCAAGGAGATCCGCTAAGGATGCTCAAAGTCTTC
CTAACTTATTGTCACTCCATTAGTTGCACGACCC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GCTAGGCTTGAATGGTACTC; Reverse Primer - name:unspecified, sequence:TTATGGAAGACTTTGAGCATC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19340 same embryo
 EMAGE:19337 same embryo
 EMAGE:19338 same embryo
 EMAGE:19341 same embryo
 EurExpress:euxassay_002105 same experiment
 MGI:4826939 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS