EMAGE mouse gene expression spatial database

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EMAGE:19378

Serp1 stress-associated endoplasmic reticulum protein 1 ( MGI:92638)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002193. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002193_01	euxassay_002193_02	euxassay_002193_03	euxassay_002193_04

euxassay_002193_05	euxassay_002193_06	euxassay_002193_07	euxassay_002193_08	euxassay_002193_10

euxassay_002193_11	euxassay_002193_12	euxassay_002193_13	euxassay_002193_14	euxassay_002193_15

euxassay_002193_16	euxassay_002193_17	euxassay_002193_18	euxassay_002193_19	euxassay_002193_20

euxassay_002193_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19378_wholemount_strong.wlz

19378_wholemount_moderate.wlz

19378_wholemount_weak.wlz

19378_wholemount_possible.wlz

19378_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19378_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

meckel's cartilage

moderate

regional

moderate expression: see section 03 07 08 16 17 19 20

lower jaw incisor

moderate

regional

moderate expression: see section 10 12

lower jaw molar

moderate

regional

moderate expression: see section 17

upper jaw incisor

moderate

regional

moderate expression: see section 10 12

upper jaw molar

moderate

regional

moderate expression: see section 16 17

orbito-sphenoid

strong

regional

strong expression: see section 05 20 21 moderate expression: see section 02 03 04

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6043
Entity Detected:	Serp1, stress-associated endoplasmic reticulum protein 1 ( MGI:92638)
Sequence:	sense strand is shown >T6043 GTCCGGTGGTTGGACTCTTGCCTCTCGGCCCTGCGGCCCGCTCTCCCCGGCACGCCGCCCCAAAGGCTGC TCAGACCGCCGCGATCGGCGGCTGCCGGTGTTCCAGCGCTCCGCGTCCCGGCCCAGTGGCTGTAGACGGC GTAGGCACCGCGATCAGAGCCGAGCCTCCCTCCCGGTGGCCCGGGACCGGCGAGCGAGCGAGTCCGAGAG GTTCTTGGGGCAGGTGGCGCTGCGAAAATGGTCGCCAAGCAGAGGATCCGTATGGCCAACGAGAAGCACA GCAAGAACATAACTCAGCGCGGCAACGTCGCTAAGACCTCGAGAAATGCCCCCGAAGAAAAGGCGTCGGT AGGACCCTGGTTATTGGCCCTCTTCATTTTTGTTGTCTGTGGCTCTGCAATTTTCCAGATTATTCAAAGT ATCAGGATGGGCATGTGAAGTGACTGACCTTGAGATCTTTCCATTCTCCTGTGAATTTTAACTTGAACTC ATTCCTGATGTTCGATGCCCTAGTTAAAAACCATTGAGTAAATCGGCCTGCCTCAGAATGACTTTTCATA TCCGCCTTCATGTGTCATTCCAAGGTGTCTTCAAGAGTCATTCCAGGTTTGCTAGTCCATGCCACAGTGC CTTGCAAAAGCACCACATGAATAAAGCAATAAAATTTGATTATTAAGTTCCAGTAGTAGACCATACTTAT TCAGTACAGAATGAGTTTTATATGGTTATTAAAACATGACTAATTAGATTAAATCTGTGTAGACAGGGTA TAGATTTTGTTAACCCTAATGTGTAAATGCAATTAGCTTAAATTTTGGAATCTTATGGTTTTCTATAGCT ATGCCCTTTTTACTCTTGAATTGGAAAGCACACTCCATTATAGGGACATGTAACTGTCCTATAATAAGGT GCTTTTAAATGGTCACCTACACGGCTTAGTTTTACCACAATCATTGTCACCTAAAACTTTTTAAAAGCTT CTAGGGCATATTGTAAGTAAAGATGCTTACAGCTGCATCTGTTTCTAATGATAATGTTTCTGTTACACAG CCTTGAATTTTGCATGAGTAAGTATAGTCACCTAGGGTCCCATTCGAGCCTGGATTGAACATTTTCTAAC TTAATCAAAGTGATTGCAGTTCCACTAAAAGCTATCATGGTGAAATGAAATTAGAGATGGTTTGCTGATG TCATACTGATCAGAATTATCAAAGGGTTTACTGTGTAGATACGGAGAAAGACAAGAACTAAATGCCTTGT CGAAACATGTGGAGTCCCGTGTGCCATATGATATCTTAGCATGTTAATTGCACTTTTAATACCAAAAAGG CACATCAACTAAAGTTTTGATTGAAATGCCCTTAGGGTTCTAGGTCTATCGATCTAAATGAGCATTTGGA ATGAAATGAATTTACTGATGAGGATCAGTCTAGTCTTCCTTTGTATGACTTCGTAGGTTGTGATTGATCA TTTTCTTTTTACTAATAGTAAGGGTGAGAGTAATGAGGCAGGTTTGACATTTTCTGGTACTATTGAAAAC TACAATTAGTCCTATTTATGCACTTAGCCAGAACTTGGTGCAGACACCAAGCAATATCTGTTTATGAATG GAGAGAAGCCTGCTTATGTTTGCTTTGTTATTTGCCTCAGGAGATCTCTTTTGAAATACGCTGTTTTCAA AGGAACAGAAGGGAAACTTGCCCCTGTTCTGAGAGCAGTGTAAACCTCACAGGGCTTTGGATCCCCTCGG ACCCCCTTGGACACAGAGGAGAAGGAAGGGAAGGAGTGGGTCGGGATGCACAGGAATCTTTCCTGTATCG GACCAGGAAAGAAAGCTGGCTGTGGGTCTGTAGGGTCATGACTGTTGTCTGCCTCTTTTCACCCTGTAAG TGAATCCCTGAACTTGGTTCCCTCTGAAGCCAGTCTTGGTTAACTAATTCTATTTTACTATAACTTTAAA AGTGAAAGATATTCTCTAAATTTATAGCAGGTGCCTTATTTCCTTTGAATCAAATCATTCCATACCAGAA TTTCCCTTGGTTTACTATAGATAATTGGCTTTAAGATATTCTTGTTTTTAAACATACAATGTTTTAATTA TTTTGTTAAATTGCTGTAGCCATCAATCATTTCACATTTGTAAAATTACATTCCCTTTGTATCATGTAAT TTACCTATTCAGTGCATTTTATATTCAGGTTGGATTATGCATATTTGGGTCAATGAAAGTTGTGTCTGTT TGATTTATGGTTTAAAAATAAAGTTCCCTGGATATTTGATGCTGTTGTAAAAGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3489738 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3489738 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19381 same embryo
	EMAGE:19379 same embryo
	EMAGE:19382 same embryo
	EMAGE:19380 same embryo
	EMAGE:19377 same embryo
	EurExpress:euxassay_002193 same experiment
	MGI:4827971 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source