EMAGE mouse gene expression spatial database

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EMAGE:19381

4632428N05Rik RIKEN cDNA 4632428N05 gene ( MGI:1921298)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002156. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002156_01	euxassay_002156_02	euxassay_002156_03	euxassay_002156_04

euxassay_002156_05	euxassay_002156_06	euxassay_002156_07	euxassay_002156_08	euxassay_002156_09

euxassay_002156_10	euxassay_002156_11	euxassay_002156_12	euxassay_002156_13	euxassay_002156_14

euxassay_002156_15	euxassay_002156_16	euxassay_002156_17	euxassay_002156_18	euxassay_002156_19

euxassay_002156_20	euxassay_002156_21	euxassay_002156_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19381_wholemount_strong.wlz

19381_wholemount_moderate.wlz

19381_wholemount_weak.wlz

19381_wholemount_possible.wlz

19381_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19381_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6026
Entity Detected:	4632428N05Rik, RIKEN cDNA 4632428N05 gene ( MGI:1921298)
Sequence:	sense strand is shown >T6026 CAGCCGGCTCCCTGGGCTCCTGGAGTCCCGCTTGCTCCAAGCGCACTCCAGCAGTCTCTTTCTGCTCTTG CCCGGCTCGACGGCGACATGGGTGTCCCCGCGGTCCCAGAGGCCAGCAGCCCGCGCTGGGGAACCCTGCT CCTTGCTATTTTCCTGGCTGCATCCAGAGGTCTGGTAGCAGCCTTCAAGGTCACCACTCCATATTCTCTC TATGTGTGTCCCGAGGGACAGAATGCCACCCTCACCTGCAGGATTCTGGGCCCCGTGTCCAAAGGGCACG ATGTGACCATCTACAAGACGTGGTACCTCAGCTCACGAGGCGAGGTCCAGATGTGCAAAGAACACCGGCC CATACGCAACTTCACATTGCAGCACCTTCAGCACCACGGAAGCCACCTGAAAGCCAACGCCAGCCATGAC CAGCCCCAGAAGCATGGGCTAGAGCTAGCTTCTGACCACCACGGTAACTTCTCTATCACCCTGCGCAATG TGACCCCAAGGGACAGCGGCCTCTACTGCTGTCTAGTGATAGAATTAAAAAACCACCACCCAGAACAACG GTTCTACGGGTCCATGGAGCTACAGGTACAGGCAGGCAAAGGCTCGGGGTCCACATGCATGGCGTCTAAT GAGCAGGACAGTGACAGCATCACGGCTGCGGCCCTGGCCACCGGCGCCTGCATCGTGGGAATCCTCTGCC TCCCCCTTATCCTGCTGCTGGTCTATAAGCAGAGACAGGTGGCCTCTCACCGCCGTGCCCAGGAGTTGGT GAGGATGGACAGCAGCAACACCCAAGGAATCGAAAACCCAGGCTTCGAGACCACTCCACCCTTCCAGGGG ATGCCTGAGGCCAAGACCAGGCCGCCACTGTCCTATGTGGCCCAGCGGCAACCTTCGGAGTCAGGACGGT ACCTGCTCTCTGACCCCAGCACACCTCTGTCGCCTCCAGGCCCTGGGGACGTCTTTTTCCCATCCCTAGA TCCAGTCCCTGACTCCCCTAACTCTGAAGCCATCTAAACCAGCTGGGGAACCATGAACCATGGTACCTGG GTCAGGGATATGTGCACTTGATCTATGGCTGGCCCTTGGACAGTCTTTTAGGCACTGACTCCAGCTTCCT TGCTCCTGCTCTGAGCCTAGACTCTGCTTTTACAAGATGCACAGACCCTCCCCTATCTCTTTCAGACGCT ACTTGGGGGGCAGGGAGAAGATGTTGGATTGCTCATTGCTGTTCTCAAGATCTTGGGATGCTGAGTTCTC CCTAGAGACTTGACTTCGACAGCCACAGATGTCAGATGACCTGCATCCTATGAACGTCCGGCTTGGCAAG AGCCTTTCTTCATGGAAACCAGTAGCCCGGAGGGGATGAGGTAGGCACCTTGCCACCCTCCCGGGAGAGA GACACAAGATGTGAGAGACTCCTGCTCACTGTGGGGGTGTGGCTGGCCTGCTTGTTTGCCTGAGGATGCT CCTCTGTTGGACTGACTCTATCCCCCTGGATTCTGGAGCTTGGCTGGCCTATGTCCCACCAGAGGAGCAT CTCAGCAGCCTTCCACCAGCAACCTGAGGGCCTGCCAGCTTCGTGGCTCTGGGCTCTCATTACCTGTATG GCCGTCCACAGAGCTCAGTGGCCAGAGGCTTTGAAACAGGAAGTACATGTCAGGTTCAGGAACCACTGTG AGCTCATTAGTGTCTTGAGCAATGTGAGGCCTGGACCAGTGGACACGGAGGGAGGGTGGCGAGAGGATGA TGGGGATGATGAGGGGAACACGCTCCCTTCCTGTCCTTGTCATCCACCACTACCACTATTCAGTGTGGAG CAGTGGCAAAGGTGACCGACCTCCACAATGTCCTAGTGATGCTGGACCATTTCTAAGTGTGAAAGAGATG CTATTAAAAACAGTATGTGGCAATGGCTGCCAACAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3488482 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3488482 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19379 same embryo
	EMAGE:19378 same embryo
	EMAGE:19382 same embryo
	EMAGE:19380 same embryo
	EMAGE:19377 same embryo
	EurExpress:euxassay_002156 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source