EMAGE mouse gene expression spatial database

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EMAGE:19476

Slc35b1 solute carrier family 35, member B1 ( MGI:1343133)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005005. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005005_01	euxassay_005005_02	euxassay_005005_03	euxassay_005005_04

euxassay_005005_05	euxassay_005005_06	euxassay_005005_07	euxassay_005005_08	euxassay_005005_09

euxassay_005005_10	euxassay_005005_11	euxassay_005005_12	euxassay_005005_13	euxassay_005005_14

euxassay_005005_15	euxassay_005005_16	euxassay_005005_17	euxassay_005005_18	euxassay_005005_19

euxassay_005005_20	euxassay_005005_21	euxassay_005005_22	euxassay_005005_23	euxassay_005005_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19476_wholemount_strong.wlz

19476_wholemount_moderate.wlz

19476_wholemount_weak.wlz

19476_wholemount_possible.wlz

19476_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19476_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 05 06 07 08 22 23 24 moderate expression: see section 09 10 20 21

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 17 18 19 weak expression: see section 16

mandible

strong

regional

strong expression: see section 08 moderate expression: see section 03 04 05 06 07 09 17 18 19 20 21 22 23

lower jaw incisor

strong

regional

strong expression: see section 11 12 moderate expression: see section 15 16

maxilla

strong

regional

strong expression: see section 08 moderate expression: see section 05 06 07 17 18 19 20 21

upper jaw incisor

strong

regional

strong expression: see section 11 moderate expression: see section 15 16

orbito-sphenoid

strong

regional

strong expression: see section 03 04 20 21 22 moderate expression: see section 01 02 23 24

clavicle

moderate

regional

moderate expression: see section 10 11 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6551
Entity Detected:	Slc35b1, solute carrier family 35, member B1 ( MGI:1343133)
Sequence:	sense strand is shown >T6551 CTTTCGCCTTCGCCGCCGGTACCGCTACCTGCTCTGAACGGATCTTCGGCCGACTCGTCCGTGCGTCTCA TGGCCGCTAGTAGATCCCTGGTGCCTGATCGGCTGCGCCTGCCGCTCTGCTTCTTGGGTGTCTTTGTCTG CTATTTCTACTATGGGATCCTGCAGGAGAAGATCACAAGAGGGAAGTATGGAGAAGGACCCAAACAGGAG ACATTCACCTTTGCCTTAACTTTGGTTTTCATCCAGTGTGTGATCAATGCTATGTTTGCCAAGATCTTGA TCCAGTTTTTTGACACTGCCAGGGTGGATCGCACTCGGACCTGGCTCTATGCTGCCTGTTCTGTCTCCTA TGTGGGTGCCATGGTCTCCAGCAACTCAGCCCTGCAGTTTGTCAACTATCCAACTCAGGTTCTCGGTAAA TCCTGCAAGCCAATCCCAGTTATGCTCCTGGGAGTGACGCTCTTGAAGAAGAAGTACCCGCTGGCCAAGT ACCTGTGTGTATTGCTAATTGTGGCTGGCGTGGCTCTTTTCATGTATAAGCCCAAGAAGGTGGTTGGGAT AGAAGAGCACACTGTTGGCTTTGGAGAGCTTCTTCTGCTCATGTCTCTGACCCTGGATGGACTGACAGGT GTTTCCCAGGACCATATGCGGGCTCATTACCAAACAGGTTCCAACCACATGATGTTGAACATCAACCTTT GGTCCACGTTCTTGCTTGGTGCTGGGATCCTGTTTACTGGGGAGCTCTGGGAGTTCTTGAGCTTCGCCGA GAGGTACCCGACCATCATCTATAACATCCTGCTCTTTGGCCTGACCAGTGCCTTGGGTCAGAGCTTTATC TTCATGACAGTCGTGTACTTCGGTCCCCTGACCTGCTCCATCATCACCACAACCCGGAAGTTCTTCACCA TCTTGGCTTCTGTGATCCTCTTTGCCAACCCCATCAGCTCCATGCAGTGGGTGGGCACCGTGCTGGTTTT CCTGGGTCTCGGTCTTGATGCCAAGTTTGGGAAAGGAACAAAGAAGACCTCCCACTAGGAAGAAAGAGGC TTCCTCTACTCCAGAAACACTTAAATTATTATCTCGAACAGTGGCATCTTGGGAAAATGGACTCGGGTCA TGATAAGGGATTGGGTTCCAATCTTTTTATAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3592141 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3592141 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19478 same embryo
	EMAGE:19477 same embryo
	EMAGE:19479 same embryo
	EMAGE:19475 same embryo
	EMAGE:19480 same embryo
	EurExpress:euxassay_005005 same experiment
	MGI:4828197 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source