EMAGE mouse gene expression spatial database

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EMAGE:19478

Erlec1 endoplasmic reticulum lectin 1 ( MGI:1914003)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004847. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004847_01	euxassay_004847_02	euxassay_004847_03	euxassay_004847_04

euxassay_004847_05	euxassay_004847_06	euxassay_004847_07	euxassay_004847_08	euxassay_004847_09

euxassay_004847_10	euxassay_004847_11	euxassay_004847_12	euxassay_004847_13	euxassay_004847_14

euxassay_004847_15	euxassay_004847_16	euxassay_004847_17	euxassay_004847_18	euxassay_004847_19

euxassay_004847_20	euxassay_004847_21	euxassay_004847_22	euxassay_004847_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19478_wholemount_strong.wlz

19478_wholemount_moderate.wlz

19478_wholemount_weak.wlz

19478_wholemount_possible.wlz

19478_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19478_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 06 07 08 23 weak expression: see section 09 10 21 22

mandible

moderate

regional

moderate expression: see section 05 06 07 08 09 11 15 16 17 18 19 20 21 22

maxilla

moderate

regional

moderate expression: see section 05 06 07 08 09 11 15 16 17 18 19 20 21

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6548
Entity Detected:	Erlec1, endoplasmic reticulum lectin 1 ( MGI:1914003)
Sequence:	sense strand is shown >T6548 TCCTCCTCCCCTCCTCCTGTTCTCCTCCGGTAGAGGAGGTTGTGACCGTGGCTGGAGGACGCGGCAATGG AGGAAGGAGACGGCGGCTTGCGGAGCCTGGTCCCCGGAGGGCCACTGTTGCTGGTTCTGTACGGTCTCCT GGAGGCGTCCGGCGGCGGCCGAGCGCTGCCCCAGCTCAGCGACGACATCCCCTTCCGAGTCAACTGGCCT GGTACCGAGTTCTCTCTGCCTACAACTGGAGTTTTGTATAAAGAAGATAATTACATCATCATGACAACTG CTCATAAAGAAAAATACAAATGTATACTTCCACTTGTGACAAGTGGGGATGAGGAAGAAGAAAAGGATTA CAAAGGCCCCAATCCAAGAGAGCTTTTGGAACCACTATTTAAACAAAGCAGTTGTTCCTACAGAATTGAA TCTTACTGGACTTATGAGGTCTGTCATGGGAAACATATTCGACAGTACCATGAAGAAAAAGAAACTGGCC AAAAAGTAAATATTCATGAATACTACCTTGGGAATATGTTGGCTAAGAATCTTCTGTATGAAAAAGAACG AGAAGCAAAAGAAAATGAAAAGTCAAATGAGATTCCCACTAAAAACATTGAAGGTCAGATGACACCCTAC TATCCTGTTGGAATGGGAAATGGTACACCTTGTAGTCTGAAACAAAATCGACCCAGATCAAGTACTGTGA TGTACATATGCCATCCTGAATCTAAGCATGAGATCCTTTCAGTAGCTGAAGTTACAACTTGTGAATATGA AGTTGTCATTTTGACACCACTCTTGTGCAGTCATCCTAAATACAAGTTCAGAGCCTCTCCTGTGAATGAC ATATTCTGTCAATCACTGCCGGGCTCTCCATTTAAACCCCTCACACTAAGACAGTTGGAGCAGCAGGAAG AGATACTGAGAGTGCCATTCAGGAGAAATAAAGAGGAAGATTTGCCATCTGCTAAGGAAGAGAGATTTCC AGCCATCCACAAACCTATTGCTGTTGGCTCTCAGCCAGTGCTCACTGTTGGAACAACCCACATTTCCAAG CTGACAGATGACCAGCTCATCAAGGAGTTCCTAAGTGGCTCTTACTGCTTTCATGGGGGTGTTGGTTGGT GGAAATATGAATTCTGCTATGGCAAACATGTACATCAATATCACGAGGACAAAGATAATGGAAAAACTTC TGTAGTTGTGGGGACATGGAACCAAGAAGAGCATGTTGAGTGGGCTAAGAAAAATACTGCTAGAGCGTAT CATCTTCAAGACGATGGCACCCAGACAGTCAGGATGGTGTCACATTTTTATGGAAATGGGGATATTTGTG ATATAACTGACAAACCCAGACAAGTGACTGTAAAACTAAAGTGTAAAGAATCAGACTCTCCTCATGCTGT CACTGTGTATATGCTAGAGCCTCACTCGTGCCAGTATATTCTTGGGGTTGAATCTCCAGTAATCTGTAAA ATTCTAGATACAGCTGATGAAAATGGACTTCTTTCTCTCCCCAATTAAAAGATATTAAAGTTGAAAGAAA GAAAGATGATTGAAAATCATCACTTTCTCCTGACCACATCTCTTTGTAGAGTCTCACCATTGGACCTTGT CTGTAGTATCACAAAAAGGACTGGCAACCACACTGTGAAATTCACGTGTATATAAGAGATTACAGATAAA CTTGTAAGGCAGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3591901 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3591901 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19477 same embryo
	EMAGE:19479 same embryo
	EMAGE:19475 same embryo
	EMAGE:19480 same embryo
	EMAGE:19476 same embryo
	EurExpress:euxassay_004847 same experiment
	MGI:4824613 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source