EMAGE mouse gene expression spatial database

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EMAGE:19480

Zfp94 zinc finger protein 94 ( MGI:107610)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004873. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004873_01	euxassay_004873_02	euxassay_004873_03	euxassay_004873_04

euxassay_004873_05	euxassay_004873_06	euxassay_004873_07	euxassay_004873_08	euxassay_004873_09

euxassay_004873_10	euxassay_004873_11	euxassay_004873_12	euxassay_004873_13	euxassay_004873_14

euxassay_004873_15	euxassay_004873_16	euxassay_004873_17	euxassay_004873_18	euxassay_004873_19

euxassay_004873_20	euxassay_004873_21	euxassay_004873_22	euxassay_004873_23	euxassay_004873_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19480_wholemount_strong.wlz

19480_wholemount_moderate.wlz

19480_wholemount_weak.wlz

19480_wholemount_possible.wlz

19480_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19480_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6546
Entity Detected:	Zfp94, zinc finger protein 94 ( MGI:107610)
Sequence:	sense strand is shown >T6546 GAGCATTGCGCGTGCTTCTGTTGTGCGGGCTGATGCGAAGCATTCTGGGAAGTGTCTCCCAGGTGGTTTC TGTTAGCATCCTCTCCCCTTCTCCTCCCTTGAGTCTCCTCTCTCCCACACCTTCCTCGACTGATGCCTGG ATCTGGAGCCTGATCTCTCAACAAGGACTGAGTGGAAGGTGAGGTCCTGACTCCTTCAGCAAGGCTCCTG AAGCAGCAGAGAGACAAAGCTTGGGATGTTGGCTCTGGGGTGAGGCTGGGTTCAGGAACCCCACCTGCCT CTGCTTTCACCTGCGCGTGGGTGCAACAGCTGCCATCTTTGTTGGAGATGGTGACCTTCAGGGACGTGGC CGTGGTCTTCAGCGAGGAGGAGCTGGGGCTGCTGGACGCCGCTCAGAGGAAGCTGTACCACGATGTGATG CTGGAGAACTTCAGGATGCTGCTCTCAGTGGGAGACAAGAATCCTGAGGAGATGGAGTCTCTTGAGGAAG TGGGATTGAGGCACCTGTCACACGAGGCGCTTTTCTGCTCACAAATCTGGCAGCAGGTTAGCAGGGACCT AACGAAGGCTGGAGACTGCAGAGTAAGCATCAGGGAAACTGGCTCTCCGTTGAAACGAGATGATGCACGT GGTGCAGACAGGAAGTACAGCAAACATTCCGGTCAGAAACCATGGCTCCAACTCCACTGTGGAACAGACG GTGGTGAAGAACCTTACAGAGAGGAGAGGAGCGAGAAAGACTCTTGGGACTCTCCTCTGCAGGCTAACGG GCGACCCCCCGCAGGAGAGAAGAGGTATAGGTGTGAAAAATGTGACCACGCCTTCTGTCGGTTATCGGGT CTCCAAGCCCATCAGGTGAGGCACACCGGAGAGAAGCCATACAAGTGTGAGGAGTGTGGCAAGGGCTTCA CTCGGGCCTCCACCCTTCTGGACCATCAGCGGGGCCACACTGGAAACAAGCCCTATCAGTGCCATGCTTG CTGGAAGAGTTTCTGTCACAGCTCGGAGTTTAACAATCACATACGAGTCCACACGGGAGAGAAACCCTAT GTATGCGAGGAGTGTGGGAAAGGCTTCAGCCAGGCCTCCCATCTCCTGGCCCATCAGAGAGGCCACACTG GAGAGAAACCCTACAAATGTAGCACGTGTGGGAAGGGCTTCAGCCGGAGTTCAGATCTCAACGTTCACTG CAGAATCCACACGGGAGAGAAACCCTATAAGTGTGAGACGTGTGGGAAAGCCTTTAGTCGGGTCTCCATA CTCCAGGTGCACCAGAGGGTCCATAGTGAAGACAAACCGTACCAGTGCGCAGAGTGTGGGAGGGGCTTCA CTGTAGAGTCACACCTTCAAGCGCACCAGCGCTCCCACACCGGAGAGAGACCCTATCAATGTGAGGAGTG CGGGAGAGGCTTCTGTCGGGCCTCCAACTTTCTGGCTCATCGTGGAGTCCACACAGGCGAGAAACCGTAT CGATGTGACATATGCGGAAAACGCTTCCGGCAGAGGTCCTACCTCCATGACCACCACAGGATCCACACGG GAGAGAAACCTTACAAATGTGAGGAGTGTGGGAAAGTCTTCAGTTGGAGCTCATACCTCAAGGCCCACCA GAGAGTTCACACAGGAGAGAAACCATACAGATGTGAGGAGTGTGGGAAGGGTTTCAGCTGGAGCTCAAGT CTCCTAATTCACCAGCGCGCCCATGCAGAGGATGAGGGTCGCAAGGATCTGCCTGCATCAGAAGGCTCAC AGGGGAAACAAACTCTATAATGTTTTATAGTTTGATATTTCAACTGGAAGCTGAAAGGTCCCAACTCTTA GAGTTCTCAAGGGAGTTGTAGTATTAGTATTTTGACTGGACCAGGATGCATTTTGATGCTTGGGAGACCA CAAGCAGAGAAGCCTTGTAAGTTGGAGCCATGTAGACTTCAGTCAGAACCTTGACCTTTAACACATGGAG ACAGTAACCAACCAGGGGAAAGTTGAGTGTGACCTGGAGGTATCTCAAAGGAAGGAACCAGATCACTTTC TTATGTTGCTGGTTGCAACCCCGTGTGGGGCTTGATTACCGTAAGTGACAGTCACAAGATATTTAGGAAC ACTACAAGCTTTCTGCATATCCAGGGACCAAAACCTCAGAATTCAACAGGTTCTAGGAGCTTCTGGCAGC TCTTACCCATGATGCAACTGTGTGACTTCACTGCAGCCTTCCTCAGAACACACCATCACCACACACAGGG CCCAGCCTCTCTGCTCAGGGCCGGCGTTGTTGTCTATGACAAATTGCATGGCTTTCTACAGTACACATGC TTTCAGTTCTTCCGGAAGCGTTGCTCACGGAGCTTTCCTGTTCTCGGTCTTCTAGTTCAGCTGCAGACTC TGTTGTGTGTCTCAGTGGGATTTTGACCATCCTCATTACTCCCCTTTACAGAAAGTGTTCCACGGCACTA CTGTGCAAGTTAGGTTTTGTGGTTAATAAATTACCACTTACTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3591835 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3591835 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19478 same embryo
	EMAGE:19477 same embryo
	EMAGE:19479 same embryo
	EMAGE:19475 same embryo
	EMAGE:19476 same embryo
	EurExpress:euxassay_004873 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source