EMAGE mouse gene expression spatial database

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EMAGE:19500

Myoz1 myozenin 1 ( MGI:1929471)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004885. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004885_01	euxassay_004885_02	euxassay_004885_03	euxassay_004885_04

euxassay_004885_05	euxassay_004885_06	euxassay_004885_07	euxassay_004885_08	euxassay_004885_09

euxassay_004885_10	euxassay_004885_11	euxassay_004885_12	euxassay_004885_13	euxassay_004885_14

euxassay_004885_15	euxassay_004885_16	euxassay_004885_17	euxassay_004885_18	euxassay_004885_19

euxassay_004885_20	euxassay_004885_21	euxassay_004885_22	euxassay_004885_23	euxassay_004885_24

euxassay_004885_25	euxassay_004885_26	euxassay_004885_27

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19500_wholemount_strong.wlz

19500_wholemount_moderate.wlz

19500_wholemount_weak.wlz

19500_wholemount_possible.wlz

19500_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19500_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 18 19 20 21 22 weak expression: see section 23 24

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6893
Entity Detected:	Myoz1, myozenin 1 ( MGI:1929471)
Sequence:	sense strand is shown >T6893 CAATTCCCTGCCACTCCACAACATTCCGTTTCTGTCTAAGAGGATTTCCAGGTTATTGGACGGTGGGGTT AAGAAGTTCTAGGTACTGCAGAAGGTCTTGGACAGACACTTAGTCCTCCGAAGCTGCTGGACCCCGGGGA GAGCCGACCACCAACTGAGCAGCTGGTCAGATCCACCTCCACCATGCCACTCTCAGGAACCCCGGCCCCT AACAAGAGGAGGAAGTCAAGCAAACTGATTATGGAGCTCACTGGAGGTGGCCGGGAGAGCTCAGGCCTGA ACCTGGGCAAGAAGATCAGTGTCCCAAGGGATGTGATGTTGGAGGAGCTGTCCCTTCTTACCAACCGAGG CTCCAAGATGTTCAAGCTACGGCAGATGCGGGTGGAGAAATTTATCTATGAGAATCACCCCGATGTTTTC TCTGACAGCTCAATGGATCACTTCCAGAAGTTTCTTCCCACAGTGGGAGGACAGCTGGAGACAGCTGGTC AGGGCTTCTCATATGGCAAGGGCAGCAGTGGAGGCCAGGCTGGCAGCAGTGGCTCTGCTGGACAGTATGG CTCTGACCGTCATCAGCAGGGCTCTGGGTTTGGAGCTGGGGGTTCAGGTGGTCCTGGGGGCCAGGCTGGT GGAGGAGGAGCTCCTGGCACAGTAGGGCTTGGAGAGCCCGGATCAGGTGACCAGGCAGGTGGAGATGGAA AACATGTCACTGTGTTCAAGACTTATATTTCCCCATGGGATCGGGCCATGGGGGTTGATCCTCAGCAAAA AGTGGAACTTGGCATTGACCTACTGGCATACGGTGCCAAAGCTGAACTCCCCAAATATAAGTCCTTCAAC AGGACAGCAATGCCCTACGGTGGATATGAGAAGGCCTCCAAACGCATGACCTTCCAGATGCCCAAGTTTG ACCTGGGGCCTCTGCTGAGTGAACCCCTGGTCCTCTACAACCAGAACCTCTCCAACAGGCCTTCTTTCAA TCGAACCCCTATTCCCTGGTTGAGCTCTGGGGAGCATGTAGACTACAACGTGGATGTTGGTATCCCCTTG GATGGAGAGACAGAGGAGCTGTGAAGTGCCTCCTCCTGTCATGTGCATCATTTCCCTTCTCTGGTTCCAA TTTGAGAGTGGATGCTGGACAGGATGCCCCAACTGTTAATCCAGTATTCTTGTGGCAATGGAGGGTAAAG GGTGGGGTCCGTTGCCTTTCCACCCTTCAAGTTCCTGCTCCGAAGCATCCCTCCTCACCAGCTCAGAGCT CCCATCCTGCTGTACCATATGGAATCTGCTCTTTTATGGAATTTTCTCTGCCACCGGTAACAGTCAATAA ACTTCAAGGAAATGAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3967841 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3967841 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19499 same embryo
	EMAGE:19501 same embryo
	EMAGE:19503 same embryo
	EMAGE:19504 same embryo
	EMAGE:19502 same embryo
	EurExpress:euxassay_004885 same experiment
	MGI:4826572 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source