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EMAGE:19502

Dnajb2 DnaJ (Hsp40) homolog, subfamily B, member 2 ( MGI:1928739)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19502 EMAGE:19502 EMAGE:19502 EMAGE:19502 EMAGE:19502
"Pseudo-wholemount" of euxassay_005046. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005046_01 euxassay_005046_02 euxassay_005046_03 euxassay_005046_04
EMAGE:19502 EMAGE:19502 EMAGE:19502 EMAGE:19502 EMAGE:19502
euxassay_005046_05 euxassay_005046_06 euxassay_005046_07 euxassay_005046_08 euxassay_005046_09
EMAGE:19502 EMAGE:19502 EMAGE:19502 EMAGE:19502 EMAGE:19502
euxassay_005046_10 euxassay_005046_11 euxassay_005046_12 euxassay_005046_13 euxassay_005046_14
EMAGE:19502 EMAGE:19502 EMAGE:19502 EMAGE:19502 EMAGE:19502
euxassay_005046_15 euxassay_005046_16 euxassay_005046_17 euxassay_005046_18 euxassay_005046_19
EMAGE:19502 EMAGE:19502 EMAGE:19502 EMAGE:19502 EMAGE:19502
euxassay_005046_20 euxassay_005046_21 euxassay_005046_22 euxassay_005046_23 euxassay_005046_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19502Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19502_wholemount_strong.wlz
19502_wholemount_moderate.wlz
19502_wholemount_weak.wlz
19502_wholemount_possible.wlz
19502_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19502_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 04 21
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 06 07 17 18
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 15 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6895
Entity Detected:Dnajb2, DnaJ (Hsp40) homolog, subfamily B, member 2 ( MGI:1928739)
Sequence:sense strand is shown

>T6895
GCTGACGCATGCTTGCGCACACAACCCAGCCTGGCGTGTCGTACGCAGTGGCCGCGAGCCGGGCAGCGGC
CTGGAGGAGGGACGTCTGACGCTGAGTTGCCATGGCATCCTACTACGAGATTCTAGACGTACCGCGGAGT
GCGTTCCCTGATGACATCAAGAAGGCGTACCGAAAGAAGGCTCTGCAGTGGCACCCAGACAAGAACCCGG
ATAATAAAGAATTTGCTGAAAAAAAATTTAAGGAGGTGGCAGAGGCCTATGAAGTACTATCTGACAAGCA
CAAACGGGAGATCTATGACCGCTATGGCCGGGAAGGGCTGACCGGGGCAGGAAGTGGTCCTTCTCGATCG
GAAACTGGTGGTGCGGGGCCTGGCTTCACATTCACCTTCCGTAGCCCCGAGGAAGTCTTCCGGGAGTTCT
TCGGGAGCGGAGACCCTTTTTCAGAGCTCTTTGATGACTTGGGTGTCTTCTCGGAGCTTCAGAACCAGGG
TCCCCGACTCACGGGCCCTTTCTTCACTTTCTCTTCTTCCTTTCCTGCCAACTCCGATTTCTCCTCCTCA
TCTTTCTCCTTCAGCCCGGGGGCTGGTGCTTTCCGCTCCGTTTCTACGTCCACCACCTTTGTCCAAGGCC
GCCGCATCACCACACGCAGAATCATGGAGAACGGGCAGGAGCGGGTAGAAGTGGAAGAGGATGGACAACT
GAAGTCAGTGTCAATCAATGGTGTCCCAGATGACCTGGCACTAGGCTTGGAGCTGAGCCGTCGTGAGCAG
CAACCTTCAGTTGCCCCTGGGCTGGGGGTCATGCAGGTCCGGCCGACCTCTCTCTCTCGTCCCCCTGACC
ATGATCTTTCTGAGGATGAGGACCTGCAGCTCGCCATGGCTTACAGCCTGTCAGAGATGGAGGCGGCTGG
GCAGAAGCCAGCAGATGTGTTCTGAGCTGGATATCCTGGATACAGAGTTGCTGCAGTTCCAACAGGACAG
CGCCCTCCCCAGTGTGCTAGGAGGGAACACTACATTCCTCTCCCTCATTTATGCTGAGTGTAGAGCTGGG
GGTTGGGGTGGTGGGTGGAGACTGAGTGGGAAGCACCTATAGTCTTAGCCTCTGAACTCTCATCCATGGG
TTCTTAGCGCTGTCTTTTGCGGACTACAAGTCCCAGAATGCAATGCACCCTGGACTTGGCTCTTGTAACA
CTTATTCCAGGGGAAGAGCTGCTCAGTTACAAAGCTGAGTTACTTGGGATATGTAGTTCCAACCCCGGCC
AGTCAGTCACTCTCTGAATGGGGAGATCTTGTTTTTCATTTTGTCCCTGGCAGGGGAGGTGAGAATTGTA
GATGACCAGAACTTTAGTTCCTATGGCACTTCTTGCTAGACTCGGGTTGAAGTGAGCAGATTCCTGTAGC
ATCTGAGAGAGTTTGTTTTCCCTTGGGCAACCTGGGGGGTCCGAGGAGTAAGCTAATGGGGCAGTGGGCT
GTGTAGGTAGGGCACGGTCTTGGATGGATGCGGGCTGGCAGAACCTGCGTGGGAAAGGAGGCTGCCTTGG
CTGGAGTGTGTATGGCAAGGACTGGCATGCAGAGGGAGCGTTGGACATATTGCTTGAATGGCTTTCTTTG
ACCTCTGACCTTGCTGCCCATTCTTTCTTATCATCAATTCATCCTCCATGAGCCACCTGTCCTCCTGCCT
GGGGAGCCCAAAGCCGCATGGGGATTGGTGGAGCAGGCTATGGCCGGCCTCTGTTAACACTGAGCCTTTG
AGACCCATCAAAGCAAGATGAGCTGAGCTCAGATTGAGGGGAAACGGAGGCAAATAAAGCTGAGTTTTGA
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3968468 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3968468 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19499 same embryo
 EMAGE:19501 same embryo
 EMAGE:19503 same embryo
 EMAGE:19500 same embryo
 EMAGE:19504 same embryo
 EurExpress:euxassay_005046 same experiment
 MGI:4824345 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS