EMAGE mouse gene expression spatial database

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EMAGE:19504

Plscr3 phospholipid scramblase 3 ( MGI:1917560)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004888. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004888_01	euxassay_004888_02	euxassay_004888_03	euxassay_004888_04

euxassay_004888_05	euxassay_004888_06	euxassay_004888_07	euxassay_004888_08	euxassay_004888_09

euxassay_004888_10	euxassay_004888_11	euxassay_004888_12	euxassay_004888_13	euxassay_004888_14

euxassay_004888_15	euxassay_004888_16	euxassay_004888_17	euxassay_004888_18	euxassay_004888_19

euxassay_004888_20	euxassay_004888_21	euxassay_004888_22	euxassay_004888_23	euxassay_004888_24

euxassay_004888_25	euxassay_004888_26	euxassay_004888_27

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19504_wholemount_strong.wlz

19504_wholemount_moderate.wlz

19504_wholemount_weak.wlz

19504_wholemount_possible.wlz

19504_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19504_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6891
Entity Detected:	Plscr3, phospholipid scramblase 3 ( MGI:1917560)
Sequence:	sense strand is shown >T6891 GGGGCTTCAGGTGCCGCCCTAGAGACCCTGGGCCCGTACTGGGCGCAGCTACCTCTTCGCCTCTGCCTGT CCGTCTTTGTTTCTGTGTCTGTCTAGCTGTTCCCGAGCTTGTCCCACTCCAGAACTAAGTCTCCCCTACG CCAAAAGCCCAAGACTCCCCTCCTGATTCCCATGGCAGGCTACTTGCCCCCCAAAGGCTATGCCCCTTCA CCCCCACCTCCCTACCCCGTGCCATCTGGGTATCCAGAGCCGGTGGCTCTGCATCCTGGACCGGGACAAG CTCCAGTGCCCACCCAGGTGCCTGCCCCTGCTCCCGGCTTCGCTCTCTTCCCCTCGCCAGGCCCAGTGGC TCCAGGGCCTCCTGCTCCTTTCGTGCCATTGCCAGGGGTGCCTCCTGGCCTCGAATTCCTAGTGCAGATT GATCAAATCTTGATTCATCAGAAGGCTGAACGAGTGGAAACGTTCCTAGGCTGGGAGACCTGTAATATGT ATGAACTTCGCTCCGGAACCGGACAGCAACTGGGTCAGGCAGCTGAAGAGAGCAACTGTTGTGCCCGCCT GTGCTGTGGTGCCCGCCGACCATTTCGAATCCGCCTAGCGGACCCTGGGGACCGCGAGGTGCTCCGGCTC CTCCGCCCACTTCATTGTGGCTGCAGCTGCTGCCCCTGTGGTCTTCAGGAGATGGAAGTCCAGGCTCCAC CTGGCACCACCATTGGCCATGTGCTACAGACCTGGCATCCCTTCCTTCCTAAGTTTTCCATCCTGGATGC TGATCGCCAACCTGTTCTACGAGTTGTAGGGCCTTGCTGGACTTGTGGCTGTGGTACAGACACCAACTTT GAGGTGAAGACTAAGGATGAATCGCGAAGTGTGGGCCGCATCAGCAAGCAGTGGGGAGGGCTGCTCCGAG AAGCCCTCACAGACGCCGATGACTTTGGCCTCCAATTCCCAGTCGATCTAGATGTGAAAGTGAAGGCCGT ACTGCTGGGAGCCACGTTCCTCATCGATTATATGTTCTTCGAGAAGAGAGGAGGCGCAGGACCCTCTGCC ATCACCAGTTAGAAGCCACCTCAGGATGAGGAGACCCATCTCCTTGACCAGAATTTAAGATGGTCAGCTG CCCTGGACGTTCCCTCCTGAAGCAACCCTTTCCTTGATATACACTGCGGCGGACCGACGAGGGTGGCCGA GTGGTTTGGAGCCGTTGTGTCCCATCCCTTCCCTGCTTCTCCTGTGGCCCTGCAGAAGAGCATGTATGAG ACCTGTTCCTCCTTCTGTTCACCATCTCCAGGCAGTGCCTGTGCACACATTAGCTTTTAAACTTCCTTGC ACACTCCTTCCAGCCTTCCTCTGGGGCCTCTGCATAGGCAGGGGCATCTGGAATCCTGGACTCAAGTTTT ACCCCAGGGCTTGTGGGTAAAAGGCAAGCAGTACCAAAGATGGCAGACACCACCCTTCCCTTATGGCACT TTAGCCAATTAGTTTAGCTTCCGATTGTGGCACTCTGAGGGGATCCTTGCCTCCTCACTAATAGCTGTAG CGGTTGGGCCCCAGTGCCAACTCCCTAAGCCCCTGGGCCCTGCGGGTGCTTTCTGCAGCTTCCTGTGCCT TATTTAACCGTTAACCCCTTCCTTCCCCTACTGTAGGAAGGAGGCTGTGTCTTTGTATGTTGTACTCATA TAAACTTTGAAACTTTTTAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3967872 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3967872 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19499 same embryo
	EMAGE:19501 same embryo
	EMAGE:19503 same embryo
	EMAGE:19500 same embryo
	EMAGE:19502 same embryo
	EurExpress:euxassay_004888 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source