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EMAGE:19635

Asna1 arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) ( MGI:1928379)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19635 EMAGE:19635 EMAGE:19635 EMAGE:19635 EMAGE:19635
"Pseudo-wholemount" of euxassay_005141. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005141_01 euxassay_005141_02 euxassay_005141_03 euxassay_005141_04
EMAGE:19635 EMAGE:19635 EMAGE:19635 EMAGE:19635 EMAGE:19635
euxassay_005141_05 euxassay_005141_06 euxassay_005141_07 euxassay_005141_08 euxassay_005141_09
EMAGE:19635 EMAGE:19635 EMAGE:19635 EMAGE:19635 EMAGE:19635
euxassay_005141_10 euxassay_005141_11 euxassay_005141_12 euxassay_005141_13 euxassay_005141_14
EMAGE:19635 EMAGE:19635 EMAGE:19635 EMAGE:19635 EMAGE:19635
euxassay_005141_15 euxassay_005141_16 euxassay_005141_17 euxassay_005141_18 euxassay_005141_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19635Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19635_wholemount_strong.wlz
19635_wholemount_moderate.wlz
19635_wholemount_weak.wlz
19635_wholemount_possible.wlz
19635_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19635_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 weak expression: see section 19
thymus primordium
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 08 10 11
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 05 06 14 15
brain
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
adenohypophysis
moderate moderate
regionalmoderate expression: see section 09 10 11 weak expression: see section 12
vibrissa
moderate moderate
regionalmoderate expression: see section 05 06 17 weak expression: see section 07
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 05 14 15
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 14 15 16 17
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 03 05 06 14 15 16
spinal cord
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11
cervico-thoracic ganglion
weak weak
regionalweak expression: see section 06 13
cervical ganglion
moderate moderate
regionalmoderate expression: see section 13 weak expression: see section 06
thoracic ganglion
weak weak
regionalweak expression: see section 06 09
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 05 12 weak expression: see section 04 09 10
retina
moderate moderate
regionalmoderate expression: see section 01 02 03
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 13 14 15 16
tongue
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 10 11 12 13
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 09 10 13 weak expression: see section 12
lower jaw molar
moderate moderate
regionalmoderate expression: see section 05 06 weak expression: see section 15
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 13
upper jaw molar
moderate moderate
regionalmoderate expression: see section 05 06 weak expression: see section 15
metanephros
moderate moderate
regionalmoderate expression: see section 03 04 05 10 11 12 13
lung
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 weak expression: see section 14 15 16
trachea
moderate moderate
regionalmoderate expression: see section 10
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8616
Entity Detected:Asna1, arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) ( MGI:1928379)
Sequence:sense strand is shown

>T8616
CCGGGTCCAAGATGGCGGCGGGGGTGGCCGGGTGGGGGGTTGAAGCAGAAGAGTTCGAGGATGCACCTGA
TGTGGAGCCGCTGGAACCCACGCTTAGCAATATCATCGAGCAGCGTAGCCTTAAGTGGATCTTCGTCGGG
GGCAAGGGTGGCGTTGGTAAGACCACCTGCAGCTGCAGCCTGGCAGTCCAGCTGTCGAAGGGACGTGAGA
GTGTTCTGATCATTTCCACAGACCCAGCTCACAACATCTCAGATGCTTTTGACCAGAAGTTCTCCAAGGT
GCCTACCAAGGTCAAAGGCTATGACAACCTCTTTGCTATGGAGATAGACCCCAGCCTGGGCGTTGCAGAG
CTCCCCGACGAGTTCTTCGAGGAAGACAACATGCTGAGCATGGGCAAGAAGATGATGCAGGAGGCCATGA
GCGCCTTCCCTGGCATCGATGAAGCCATGAGTTATGCTGAGGTCATGAGGCTGGTGAAAGGCATGAACTT
CTCAGTGGTAGTGTTCGACACAGCACCCACCGGCCATACACTCAGGCTCCTGAACTTCCCGACCATCGTG
GAGAGGGGCCTGGGCCGCCTGATGCAGATCAAGAACCAGATCAGCCCCTTCATCTCACAGATGTGCAACA
TGCTGGGCCTGGGGGACATGAATGCTGACCAACTGGCCTCCAAGCTAGAAGAGACGTTGCCTGTCATCCG
ATCTGTTAGCGAACAGTTCAAGGACCCTGAACAGACAACGTTCATCTGTGTGTGCATCGCCGAATTCTTG
TCCTTGTACGAGACGGAGCGGCTGATCCAGGAGCTGGCTAAGTGCAAGATCGACACCCACAACATCATCG
TCAACCAGCTTGTCTTCCCTGACCCTGAGAAACCCTGCAAGATGTGTGAGGCCCGACACAAGATCCAGGC
CAAGTACCTGGACCAGATGGAAGACCTATATGAAGACTTTCACATTGTAAAGCTGCCACTGTTACCTCAC
GAGGTTCGGGGAGCCGACAAAGTCAACACCTTCTCTGCCCTCCTCCTGGAGCCCTACAAGCCCCCCAGCA
CCCAGTAGCCACCCCCATCCCTGCTGCTGCCTTCATACCCTCCTCTTTCCCCATGGGGCAGAGTTTGCAC
AAAGTCCCCCATAATGCAGGGGGCCACTTAGGAGGTGGGAGGGGGTCTGTTCCCCCTGGTGGGGCCTGGG
GAGGCTGCAATGCCCCCCACCTCTTCCTGCCTCACACTCCTCAATAAAATAATCTTACACAAAAAAAAAA
AAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5717763 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5717763 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19638 same embryo
 EMAGE:19636 same embryo
 EMAGE:19634 same embryo
 EMAGE:19639 same embryo
 EMAGE:19637 same embryo
 EurExpress:euxassay_005141 same experiment
 MGI:4823277 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS