EMAGE mouse gene expression spatial database

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EMAGE:19639

Fam132b family with sequence similarity 132, member B ( MGI:3606476)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006339. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006339_01	euxassay_006339_02	euxassay_006339_03	euxassay_006339_04

euxassay_006339_05	euxassay_006339_06	euxassay_006339_07	euxassay_006339_08	euxassay_006339_09

euxassay_006339_10	euxassay_006339_11	euxassay_006339_12	euxassay_006339_13	euxassay_006339_14

euxassay_006339_15	euxassay_006339_16	euxassay_006339_17	euxassay_006339_18	euxassay_006339_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19639_wholemount_strong.wlz

19639_wholemount_moderate.wlz

19639_wholemount_weak.wlz

19639_wholemount_possible.wlz

19639_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19639_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8595
Entity Detected:	Fam132b, family with sequence similarity 132, member B ( MGI:3606476)
Sequence:	sense strand is shown >T8595 AAGGCGCCGACCTGACCAGCCGTGCTCTTTACTGCCGCCGCCGCCGCCAGCATGGCCTCGACCCGCCGCC CCGTCGGAGCTCGCACGCTGCTCGCCTGCGCCAGCCTACTCGCCGCCATGGGCCTCGGTGTCCCTGAGTC CGCGGAGCCCGTGGGGACTCATGCACGCCCGCAGCCGCCCGGGGCCGAGCTGCCCGCCCCGCCAGCCAAC AGCCCGCCGGAACCCACCATTGCGCATGCACACAGTGTGGATCCCCGGGATGCTTGGATGCTGTTCGTCA AGCAGAGTGACAAGGGGATCAACAGTAAGAGGAGGAGCAAAGCCAGGAGGCTGAAGCTTGGCCTGCCAGG ACCCCCAGGGCCACCAGGTCCTCAGGGCCCCCCAGGCCCCTTTATCCCATCTGAGGTTCTGCTGAAGGAG TTCCAGCTGTTGCTGAAAGGCGCAGTACGGCAGCGAGAGAGCCATCTGGAGCACTGCACCAGGGATCTCA CTACACCAGCCTCGGGTAGCCCTTCCCGTGTCCCAGCCGCCCAGGAGCTTGATAGCCAGGACCCAGGGGC ATTGTTAGCTCTGCTGGCTGCGACCTTGGCCCAGGGCCCGCGGGCACCACGTGTGGAGGCCGCATTCCAC TGTCGCTTGCGCCGGGATGTGCAGGTGGATCGGCGTGCGTTGCACGAGCTTGGGATCTACTACCTGCCCG AAGTTGAGGGAGCCTTCCACCGGGGCCCAGGCTTGAATCTGACCAGCGGCCAGTACACCGCACCTGTGGC TGGCTTCTATGCGCTTGCTGCCACTCTGCACGTGGCACTCACCGAGCAGCCAAGAAAGGGACCAACACGA CCCCGGGATCGTCTGCGCCTGCTGATCTGCATCCAGTCTCTCTGCCAGCACAATGCCTCCCTGGAGACTG TGATGGGGCTGGAGAACAGCAGCGAGCTCTTCACCATCTCAGTAAATGGTGTCCTCTATCTACAGGCAGG ACACTACACTTCTGTCTTCTTGGACAATGCCAGCGGCTCCTCCCTCACGGTACGCAGTGGCTCTCACTTC AGTGCTATCCTCCTGGGCCTGTGAGCAGCTGCTGCAGGCTCTTACCCTTACCAAGCAGAGTGGTGACAGT GGCCCTGTGCACAAGGAAGCCCACTGGACCTCTGCACACACTGGCCATCGCAATACCTCTTACCATAGCC ACTGTATTCCTGCAGAGGTGATGGAAGCAGGCTCTCCCAGAACCAGCAGCAATTTGTGAATATCCTAAGC CCTGCTTGGCTCTTCTGGGACCATCCAGCTTACATGGTAGCACTTGCTCTATTCCCAGCTACAGAGCTGG CATCCTTGTAGCTCTATTCAGGCTCAAATTCCTTCCTGTGGCCCAGCAAACTAGTCAGGCCACTCACTAC CTGGGACAACTCAGCCTGCACAGGGTAGGAAATGACTGTAGGTCCTTAGTGCTTAAGAGGGAAGCCCAGA CCTTCTTGGACCATACACTGTCTACCTGGGTTCCTGAGTTCCTATCCTGCAGTATTCTCTATGTCTGCCT AGAGTACAGCCTCAGGGACCTAGGAACGTCAGGGGCCAGTAATCCTAGGCCTGGCAGCAGTTTCTGTGTC TGGGGTAAGCACGATGACCTGCAGGCCCCTGGTGAGCACCCTAACATACTGGAGCTACACTCCTTTGCCA TCCTTATCCTCTACAAAGGCTACCAGCCAACTTCCCAACCCTCGGAGGCTCGGGCTCACTGCTAGGGCCC ACACACTAAACCAAAGTTTGAGCACAGTTATCCACACAGGGCACCTGCTCACAAGTTTCCCTCCTGAGAC TCCATTGTAAAGGGGGTGGGTGGTCTGGCTGAATGGCAGCTTGGGAGGAAGAAGCTGGCTGAGGGCTCTT CCCATGAGAAGGGCTGCCACCCTGCAGCCTGCTTTAGTGGTTTTCCCATTTTTAACAAAATGTGGGACCA GATCCTAATAGGCAGTGGAGAGGGCTGGACCTTCCAAGTCCTGATGGCCTGCTGATGAAAGAGCTGACAG CCAGCAGTCCACACCCACCAGCTGCTTTGGGATCTCTTGGCCTAGCAGGAGTGCTCAAGCCTGGGACAGA GGGGATAGCTCCAGTTGCTTCATCTTTCAGCTGACACCAGGATGAAGCTACCACATCAGAACGCCCTTCT ATAATCGGACCTGTCCCCAAATCAGGCTTCCAACAGAGCGACAGGTGCCTGGGGGAGCTGTCCGTGAGCA GGCCTGGCCCGATTCTCAGTATGGCTGGTACCCACCCTACCAGGAAGCACTGTGCTGGCCTGGGTCCTGA CTCCCTGGCACTCTGATAAGGACATGAGGTGGAAACACCCCACCCTTTTCTGGATTTCATTTTGTATCCA GTGGGCACAGACATGTCAGTGACGTTATCTTCTACCCTTGAGAAAGCCACATAAGCACATATCTTACCTT CCAACAACTGTGTTACACAGCAACTTAAAAGTATTTATTGTGTCATATTGCTAGGCAGAATATATTTATG TAATGGATCACACAAAGCCCTAACAATGAAGGGCAGACCCGAGAAGCATGACCTCTTTCCAGCTCACTTC ACCAAAGACCATGAGGGGTAAGCTCTGGAAAGCAAGGGAGAAATGCCTTTAGTCTTGAGACTCCTACTGA AGTAGCAGGAGAGGCTCTTACTAACCAGGAAGGCCACAGTGCCAAGAGTTACCCAGACAGAAGCACTTGG GGACATGGGTCACAGCCTATGGGGCACTGAATGATTCCTGGGCTGCTATGAAATTGCTAAATTAAACTGT TTAGCTTTCTGATGAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5716432 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5716432 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19638 same embryo
	EMAGE:19635 same embryo
	EMAGE:19636 same embryo
	EMAGE:19634 same embryo
	EMAGE:19637 same embryo
	EurExpress:euxassay_006339 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source