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EMAGE:19638

Vezt vezatin, adherens junctions transmembrane protein ( MGI:2143698)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19638 EMAGE:19638 EMAGE:19638 EMAGE:19638 EMAGE:19638
"Pseudo-wholemount" of euxassay_005115. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005115_01 euxassay_005115_02 euxassay_005115_03 euxassay_005115_04
EMAGE:19638 EMAGE:19638 EMAGE:19638 EMAGE:19638 EMAGE:19638
euxassay_005115_05 euxassay_005115_06 euxassay_005115_07 euxassay_005115_08 euxassay_005115_09
EMAGE:19638 EMAGE:19638 EMAGE:19638 EMAGE:19638 EMAGE:19638
euxassay_005115_10 euxassay_005115_11 euxassay_005115_12 euxassay_005115_13 euxassay_005115_14
EMAGE:19638 EMAGE:19638 EMAGE:19638 EMAGE:19638 EMAGE:19638
euxassay_005115_15 euxassay_005115_16 euxassay_005115_17 euxassay_005115_18 euxassay_005115_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19638Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19638_wholemount_strong.wlz
19638_wholemount_moderate.wlz
19638_wholemount_weak.wlz
19638_wholemount_possible.wlz
19638_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19638_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
brain
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
facial vii ganglion
strong strong
regionalstrong expression: see section 03 15 16 17 18
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 05 06 14
trigeminal v ganglion
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 13 14 15 16 17 18 19
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 13 14 15 16
trigeminal v nerve
strong strong
regionalstrong expression: see section 13
spinal cord
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 11
cervical ganglion
strong strong
regionalstrong expression: see section 06 13
dorsal root ganglion
strong strong
regionalstrong expression: see section 04 05 06 09 10 11 12
retina
strong strong
regionalstrong expression: see section 01 02 03 moderate expression: see section 19
naris
moderate moderate
regionalmoderate expression: see section 10
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 08 09 10 12 13 14 15 16
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 10 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8593
Entity Detected:Vezt, vezatin, adherens junctions transmembrane protein ( MGI:2143698)
Sequence:sense strand is shown

>T8593
CGTGCTGAGGCGGGTGGTATGGCGGAGAAGGATGACACCGGAGTTTGACGAAGAGGTGGTTTTTGAGAAT
TCTCCACTTTACCAGTACCTACAGGATTTGGGACACACAGATTTTGAGATATGTTCTTCTTCGTCACCAA
AACCAGAAAAATGCTTGACAACAGAGGGACCTCAGCCGCCTCCCACCAGAGTCCTACAGAGACAAGGCAT
CCTGTTGAAACTGACAGAAACCATCAAGAGCTGGACGTTTTCCTCTCAGCACAGTAAGAAAGATGACTTA
CTCCATAAGTTGGACACCGGGTTTCGGCTGGACTCACTGCATACCATCCTGCAGCAGGAAGTCCTGCTGC
AAGAGGATGTGGAGCTGCTCGAGCTGCTTGACGCCAGCATCCTGTCTGCGGGGCAGCCTCAACAGGAAAG
TGGACACCTTCCAACGCTCTGCTCCCTGGCAACCCCGAACACTTGGGATGTCTCGCTGCTGTTTGCCTTC
ATTAGTTTGCTCATCATGTTTCCCACGTGCTGGATTGTGTCCTCCTGGCTGGTGTGGGGAATAATTCTAT
TTCTTTACTTGATCATACGAGTTTTGAAATTATGGAGGACAGCCAAACTGCAAATGACTCTGAAAAAATA
TAGGGTACGTTTGGAAGACATGGCAGCCAATAGCCGAGCCTTCACCAACCTTGTGAGAAAATCACTGCGT
CTCATCCAAGAAACTGAAGTCATTTCCAGGGGATTTACTCTGGTCAGTGCTGCTTGCTCATTTAATAAGG
CTGCACAGCATCCCGGCCAGCATCTCATCGGCCTTCGGAAAGCCGTCTACAGGACCGTAAGAGCCAACTT
TCAAGCCGCAAGGCTAGCTACCTTGTACATGCTGAAAAACTACCCCCTGAACTCTGAGAGTGACAATGTC
ACCAACTACATCTGTGTGGTACCTTTTAAGGAGCTGGGGCTTGGACTCAGTGAAGACCAGATCTCAGAAG
AGGAAGCTCGTAACCTGACAGATGGCTTCAGCCTGCCTGCCTTGAAGGTTTTGTTCCAGCTCTGGGTGGC
ACAGAGTTCAGAGTTCTTCAGACGCCTGGCCCTGTTACTTTCTACAGCGAATTCGCCTTCAGGGCCCTTA
CTCACTGCTGCACTTCTGCCTCATCATATCCTCTGTGACGTGACTCAAGGTCTACCTCATGCTCACTCTG
CCTGCTTGGATGAGCTGAAGCGCAGCTATGAGTTCTTCCGGTACTTTGAAACTCAGCACCAGTCAGTCCC
CCAGCGCTTATCCAAGACTCCGCAGAAGTCGAGGGAGCTGAGCAACGTCCACACAGCAGTCCGTAGCTTA
CAGCTCCATCTGAAAGCACTACTGAATGAAGTAATAATTCTTGAAGATGAACTTGAAAAGCTCGTTTGTA
CTAAAGAAACACAAGAGCTGCTATCGGAGGCTTATCCGATACTAGAGCAGAAATTAAAACTGATTGAACC
ACACGTTCAGGCCAGCAACAGTTGCTGGGAGGAAGCCATTTCTCAAGTGGATAAACTGCTCCGGAGGAAC
ACAGATAAAAAAGGCAAGCCTGGAGTAGCCTGTGAGAACCCACACTGCACCGCGGAGCCCTTGGTGCGGC
CAGCTCTACACATTGAGGACAGAGACCCCATCCCTGAGGAGCAGGAATTAGAAGCTTATGTGGATGACAT
AGACATAGAAAGTGAGTTCCGAAAGGACGATTTCTATCACTTGTCTCAGGAAGACAGAGAGAGACAGAAG
CGCGAGCAGGAGGAGTCCAGGCGGGTGCTGCAGGAGCTGAAGTCTGTGCTGGGATTTAAGGCTTCCGAGG
CTGAGAGGCAGAAGTGGAAGCAGCTGCTGTTCAGTGACCACGCTGTGTTGACATCCTTGTCTCCTGTAGA
CCCAGTGGAATCTGTAAGTAACTCAGAACCACCCATGAATTCAGATACAGAAAAAGTCAATAGTAATGCT
ACTGAAGAGGAGACTAGTAAGCCCTGTGCCGGCGACAAGGAAGACAGTAGGACTGAGTATGTGTGTGACA
GCCCAACAGAGGGGCCAAGCAAAGACACCTCTGCAGACACAGGCCTCCTCCTTCCAGGAGCCGAGGAAAC
CATGTGCCACCAGCATGAGAGTGAGGCTAAGTCTCCTCAGGCTGCTGCTGCGGGGGCCACTGCCCCTCCG
ACTCCCAGGGACACACTGCAACTCTCCATTAAGCAGAGGCTGGCTCGACTCCAGTTGCCACCAGAATTCA
CCTTCTCTGCTGGCCTGGCTGCAGAAGTGGCCGCCCGGTCCCTGTCCTTTACCACCATGCAGGAACAAAC
TTTTGGCGACGAGGAGGAAGAACAGCTAGTTGAAGGCGGTGAAAATGAGGTGGAGGAAAAATAAAAGCCA
AGATGAAGCGATGGCCTTGATTGTTCTTTTTACAAAAGTGTTCTAGCTGCAAGACTGGAAAGAGAAATGA
GTGTGTGTTTACTCTCTATAAGGCTACGGCGTGAATTTACAGGGTGAATACTGATTTGAAAACCTGACCT
GAAACTAGTAGATACCTGTCTAGTCAGATCCTTTAGGCATAGGAGAGAAAGGTAAGGGACTTTTTTAAAA
AATAAATAAATAAACTGCTGTTTTATTTGTGGCAAAACTGATAATCTTGGCAAATCTTTAAGTACTTTTA
ATAAGACATGAATTTATCACTTTTTGCCAAAACTTTGCTGCCACAAGGTAACTAGGGTGATTGTCTTCCC
GAGCAGAAACATTTAGAATGAGCCCTTTCTACTGTGTCCTTGCAGTGTGAACTGCAGCGGTCATGTTAAT
ACATTGTTCGTACTTTATTTTGTTTATACCACTCGTAAAACAAAGAGACAGGTTCTAAATAAAAGTATTT
AATTACCACAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5716245 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5716245 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19635 same embryo
 EMAGE:19636 same embryo
 EMAGE:19634 same embryo
 EMAGE:19639 same embryo
 EMAGE:19637 same embryo
 EurExpress:euxassay_005115 same experiment
 MGI:4829156 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS