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EMAGE:20246

Degs2 degenerative spermatocyte homolog 2 (Drosophila), lipid desaturase ( MGI:1917309)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20246 EMAGE:20246 EMAGE:20246 EMAGE:20246 EMAGE:20246
"Pseudo-wholemount" of euxassay_012375. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012375_01 euxassay_012375_02 euxassay_012375_03 euxassay_012375_04
EMAGE:20246 EMAGE:20246 EMAGE:20246 EMAGE:20246 EMAGE:20246
euxassay_012375_05 euxassay_012375_06 euxassay_012375_07 euxassay_012375_08 euxassay_012375_09
EMAGE:20246 EMAGE:20246 EMAGE:20246 EMAGE:20246 EMAGE:20246
euxassay_012375_10 euxassay_012375_11 euxassay_012375_12 euxassay_012375_13 euxassay_012375_14
EMAGE:20246 EMAGE:20246 EMAGE:20246 EMAGE:20246 EMAGE:20246
euxassay_012375_15 euxassay_012375_16 euxassay_012375_17 euxassay_012375_18 euxassay_012375_19
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euxassay_012375_20 euxassay_012375_21 euxassay_012375_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20246Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20246_wholemount_strong.wlz
20246_wholemount_moderate.wlz
20246_wholemount_weak.wlz
20246_wholemount_possible.wlz
20246_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20246_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
stomach
moderate moderate
regionalmoderate expression: see section 08
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31264
Entity Detected:Degs2, degenerative spermatocyte homolog 2 (Drosophila), lipid desaturase ( MGI:1917309)
Sequence:sense strand is shown

>T31264
CACTGGCCATCCATGACATCTCGCACAATACTGCCTTTGGCACAAGTTGTGTCTCCCGAAACCGCTGGTT
TGCCATCTTTGCTAATCTGCCCATTGGCCTACCTTACGCTACATCCTTCAAAAAGTACCATGTGGACCAC
CATCGTTACCTGGGTGGAGACGGGCTGGATGTGGATATTCCTACGAACTTCGAGGGCTGGTTCTTCTGCA
CGCCGGCCCGCAAGCTGCTCTGGCTGGTGCTTCAGCCTTTCTTCTATTCACTAAGGCCTCTCTGTGTGAA
CCCCAAGGTAGTGACACGTATGGAGATCCTCAACGCCCTGGTGCAGCTAGCATTTGATGTCACCATCTTT
GCCCTCTGGGGGATCAAGCCCATAGTCTACCTTCTAGGCAGCAGCCTCCTGGGTCTGGGCCTGCACCCTA
TCTCTGGTCACTTTGTGGCTGAGCATTATATGTTCCTGAAGGGCCACGAGACTTACTCCTACTATGGACC
CCTCAATTGGATCACCTTCAATGTGGGTTACCACATGGAACACCACGACTTCCCCAGTATCCCCGGTTAC
TACCTGCCACTGGTGCGGAAGATTGCACCTGAATATTATGACCACCTGCCGCAGCACCACTCCTGGGTGA
AAGTGCTCTGGGACTTCGTATTTGAGGACTCTATGGGACCCTACTCCAGGGTTAAGCGCAAGTGTAAGCT
GGCGAAGGACCACCTGTGAGCCCTGCCGACCAGTAGTCCAGATCGAGCAGCCATACGCCTCCTGCCCGTG
TGCCTGCATCAGCTTCTGCAGTCTAAGCTGCTTCTGGGTCTGTTGTGCCCAGCAAGTAGATAGATACAGA
GTAACCAGGCTTACCTGAACTTTCTGGGTCTTAGCTATGACTGACCAGGTTACGGAGCAGGCAGTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4218832), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 55705. Forward Primer - name:055705_F_IRAV49_e11_2210008A03Rik, sequence:CACTGGCCATCCATGACA; Reverse Primer - name:055705_R_SP6_IRAV49_e11_2210008A03Rik, sequence:GGACTGCCTGCTCCGTAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20248 same embryo
 EMAGE:20251 same embryo
 EMAGE:20249 same embryo
 EMAGE:20247 same embryo
 EMAGE:20250 same embryo
 EurExpress:euxassay_012375 same experiment
 MGI:4824280 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS