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EMAGE:20248

Cpa1 carboxypeptidase A1, pancreatic ( MGI:88478)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20248 EMAGE:20248 EMAGE:20248 EMAGE:20248 EMAGE:20248
"Pseudo-wholemount" of euxassay_012317. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012317_01 euxassay_012317_02 euxassay_012317_03 euxassay_012317_04
EMAGE:20248 EMAGE:20248 EMAGE:20248 EMAGE:20248 EMAGE:20248
euxassay_012317_05 euxassay_012317_06 euxassay_012317_07 euxassay_012317_08 euxassay_012317_09
EMAGE:20248 EMAGE:20248 EMAGE:20248 EMAGE:20248 EMAGE:20248
euxassay_012317_10 euxassay_012317_11 euxassay_012317_12 euxassay_012317_13 euxassay_012317_14
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euxassay_012317_15 euxassay_012317_16 euxassay_012317_17 euxassay_012317_18 euxassay_012317_19
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euxassay_012317_20 euxassay_012317_21 euxassay_012317_22 euxassay_012317_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20248Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20248_wholemount_strong.wlz
20248_wholemount_moderate.wlz
20248_wholemount_weak.wlz
20248_wholemount_possible.wlz
20248_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20248_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
pancreas
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16
ovary
moderate moderate
regionalmoderate expression: see section 06 07 08 17 18 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30475
Entity Detected:Cpa1, carboxypeptidase A1, pancreatic ( MGI:88478)
Sequence:sense strand is shown

>T30475
CCAGTGGGGTCTGGTTTGCAAAAAAGATCACGAAGGACTATGGCCAGGAACCCACCCTCACGGCCATTCT
TGACAACATGGACATCTTCCTGGAGATTGTAACTAACCCTGATGGTTTTGTCTACACCCACAAAACGAAT
CGCATGTGGCGCAAAACTCGATCACACACCGAGGGCTCCCTCTGCGTCGGGGTGGACCCCAACAGGAACT
GGGATGCTGCCTTTGGGATGCCCGGAGCTAGTAGCAACCCCTGCTCAGAAACTTACCGTGGCAAGTTTCC
CAACTCTGAAGTGGAGGTCAAGTCCATCGTGGACTTTGTGACGAGCCACGGGAACATCAAGGCCTTCATC
TCCATCCACAGCTACTCCCAGCTCCTTCTCTACCCCTATGGCTACACATCAGAACCAGCTCCTGACAAGG
AGGAGCTGGATCAGCTAGCAAAATCTGCTGTGACAGCCTTGACATCTCTACACGGGACCAAGTTCAAGTA
TGGCAGTATCATTGATACGATCTATCAAGCCAGTGGGAGCACTATTGACTGGACCTACAGCCAGGGCATC
AAGTATTCCTTCACTTTTGAGCTGAGGGACACTGGGCTCAGAGGTTTCCTGCTGCCTGCCTCCCAGATCA
TCCCCACAGCGGAGGAGACATGGCTGGCCCTTTTGACCATCATGGACCACACTGTCAAACACCCCTACTG
AAGAAGCTTTTATTCCCTTCCCCTTTCTTCTCCTTAGCCCCATTTAGGCAAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:30050094), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58857. Forward Primer - name:058857_F_IRAV107_d12_Cpa1, sequence:CCAGTGGGGTCTGGTTTG; Reverse Primer - name:058857_R_SP6_IRAV107_d12_Cpa1, sequence:GGTTGCCTAAATGGGGCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20251 same embryo
 EMAGE:20249 same embryo
 EMAGE:20246 same embryo
 EMAGE:20247 same embryo
 EMAGE:20250 same embryo
 EurExpress:euxassay_012317 same experiment
 MGI:4824022 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS