EMAGE mouse gene expression spatial database

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EMAGE:20249

Cpn1 carboxypeptidase N, polypeptide 1 ( MGI:2135874)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012370. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012370_01	euxassay_012370_02	euxassay_012370_03	euxassay_012370_04

euxassay_012370_05	euxassay_012370_06	euxassay_012370_07	euxassay_012370_08	euxassay_012370_09

euxassay_012370_10	euxassay_012370_11	euxassay_012370_12	euxassay_012370_13	euxassay_012370_14

euxassay_012370_15	euxassay_012370_16	euxassay_012370_17	euxassay_012370_18	euxassay_012370_19

euxassay_012370_20	euxassay_012370_21	euxassay_012370_22	euxassay_012370_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20249_wholemount_strong.wlz

20249_wholemount_moderate.wlz

20249_wholemount_weak.wlz

20249_wholemount_possible.wlz

20249_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20249_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 08 09 10 11 moderate expression: see section 12 13 14 15

stomach

strong

regional

strong expression: see section 05 06 07 08 09 moderate expression: see section 02 03 04

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 weak expression: see section 23

metanephros

strong

regional

strong expression: see section 08 09 10 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31262
Entity Detected:	Cpn1, carboxypeptidase N, polypeptide 1 ( MGI:2135874)
Sequence:	sense strand is shown >T31262 AACCCTTGGAACCGGAAGTCAAGTATGTGGGAAACATGCACGGGAACGAGGTGCTGGGCCGCGAGCTGCT TCTGCAGCTGTCGGAATTCCTCTGCGAGGAGTTCCGGAACCGAAACCAGCGTATCCTGCGCCTCATTCAG GACACGCGCATTCACATCCTGCCTTCCATGAACCCTGACGGCTACGAGGTGGCTGCTGCCCAGGGCCCAA ACATGTCTGGGTATCTGGTTGGTAGGAACAATGCAAATGGGGTGGACCTGAACCGCAACTTCCCGGATCT CAACACCTACTTCTACTACAACTCCAAGAACGGTGGCCCTAACCACCACCTGCCCCTCCCCGACAACTGG AAAAGTCAGGTGGAACCAGAGACCCGGGCGGTGATCCAGTGGATTCGCTCCTTGAACTTCGTTCTCTCAG CCAATATGCACGGCGGGGCTGTGGTGGCCAATTACCCATATGACAAGTCACTTGAGCATAGGTTTCGAGG TCCCCATCGCACCTCCAATTCCCCGACACCTGATGACGAGCTCTTCCAGACGCTGGCCAAGGTCTACTCC TACGCCCATGGATGGATGCACCAAGGTTGGAACTGTGGGGACTACTTTCCAGACGGCATCACCAACGGGG CGTCCTGGTATTCTCTCAGCAAGGGAATGCAAGACTTCAATTATCTCCACACTAACTGCTTTGAGATCAC GCTCGAGCTGAGTTGCGACAAGTTTCCCCGGCAAGAGGAGCTGCAGAGGGAGTGGCTGGGTAACCGGGAA GCCTTAATCCAGTTCTTGGAACAGGTCCACCAGGGCATCAAGGGAAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4211169), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 55655. Forward Primer - name:055655_F_IRAV49_e09_Cpn1, sequence:AACCCTTGGAACCGGAAG; Reverse Primer - name:055655_R_SP6_IRAV49_e09_Cpn1, sequence:CATTCCCTTGATGCCCTG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20248 same embryo
	EMAGE:20251 same embryo
	EMAGE:20246 same embryo
	EMAGE:20247 same embryo
	EMAGE:20250 same embryo
	EurExpress:euxassay_012370 same experiment
	MGI:4824029 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source