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EMAGE:20268

BC016495 cDNA sequence BC016495 ( MGI:2147434)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20268 EMAGE:20268 EMAGE:20268 EMAGE:20268 EMAGE:20268
"Pseudo-wholemount" of euxassay_012326. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012326_01 euxassay_012326_02 euxassay_012326_03 euxassay_012326_04
EMAGE:20268 EMAGE:20268 EMAGE:20268 EMAGE:20268 EMAGE:20268
euxassay_012326_05 euxassay_012326_06 euxassay_012326_07 euxassay_012326_08 euxassay_012326_09
EMAGE:20268 EMAGE:20268 EMAGE:20268 EMAGE:20268 EMAGE:20268
euxassay_012326_10 euxassay_012326_11 euxassay_012326_12 euxassay_012326_13 euxassay_012326_14
EMAGE:20268 EMAGE:20268 EMAGE:20268 EMAGE:20268 EMAGE:20268
euxassay_012326_15 euxassay_012326_16 euxassay_012326_17 euxassay_012326_18 euxassay_012326_19
EMAGE:20268 EMAGE:20268 EMAGE:20268
euxassay_012326_20 euxassay_012326_21 euxassay_012326_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20268Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20268_wholemount_strong.wlz
20268_wholemount_moderate.wlz
20268_wholemount_weak.wlz
20268_wholemount_possible.wlz
20268_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20268_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 06 07 08 16 17 18 moderate expression: see section 05 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31259
Entity Detected:BC016495, cDNA sequence BC016495 ( MGI:2147434)
Sequence:sense strand is shown

>T31259
GACCCTGCTGGGAAGGATTCGAACCCGCTTCGGATATATTAGGAAGGTGTGATTTCCAAAGCCAGTTATG
AAGAGATTTGTCATTGGAATTGGTGGTGTGACAAACGGAGGGAAGACGACACTGGCTAAGAGCTTGCAGA
AGCACCTTCCCAACTGCAGCGTCATATCTCAGGATGACTTCTTCAAGCCAGAGTCTGAGATAGACATAGA
TGAAAATGGTTTTTTGCAGTATGATGTGCTTGAAGCGCTAAATATGGAAAAAATGATGTCAGCAGTTTCC
TGTTGGATGGAAAACCCAGGAAGCTCTGCGGGACCAGCAGCCTTGGAAAGTGCTCAAGGGGTTCCCATTT
TAATTATTGAAGGTTTCCTTCTCTTTAATTATAAGCCTCTGGACACCATATGGAACAGAAGTTACTTCCT
GACCGTTCCATATGAAGAATGTAAGAGGAGAAGGAGTACCAGAGTATATGAGCCTCCAGACCCTCCAGGG
TACTTCGATGGCCACGTGTGGCCCATGTACCTAAAGCACAGACAGGAAATGAGCTCCATCACCTGGGACA
TTGTTTACCTGGATGGAACAAGGTCTGAAGAGGACCTCTTCTCTCAGGTGTATGAAGATGTCAAGCAGGA
ACTAGAGAAGCAAAATGGTTTGTGATAACACCACACAGATGGAGTGCAGCAAGTTCCCGGGATGGATTCA
GATACTTGAAGGGCTATTCTGAAGGACATTTACAGGGATGCAGTATGTCCTGCAGGACAGTGGCAGCTCG
TCTGTATGTTCACTTTTGTGCCACTGTCCCCAACATCCAGAGCCATGTGTGCCCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4221437), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 55755. Forward Primer - name:055755_F_IRAV49_e03_BC016495, sequence:GACCCTGCTGGGAAGGAT; Reverse Primer - name:055755_R_SP6_IRAV49_e03_BC016495, sequence:ATGGGCACACATGGCTCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20266 same embryo
 EMAGE:20269 same embryo
 EMAGE:20265 same embryo
 EMAGE:20267 same embryo
 EurExpress:euxassay_012326 same experiment
 MGI:4823409 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS