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EMAGE:20721

D2Wsu81e DNA segment, Chr 2, Wayne State University 81, expressed ( MGI:106544)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20721 EMAGE:20721 EMAGE:20721 EMAGE:20721 EMAGE:20721
"Pseudo-wholemount" of euxassay_013998. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013998_01 euxassay_013998_02 euxassay_013998_03 euxassay_013998_04
EMAGE:20721 EMAGE:20721 EMAGE:20721 EMAGE:20721 EMAGE:20721
euxassay_013998_05 euxassay_013998_06 euxassay_013998_07 euxassay_013998_08 euxassay_013998_09
EMAGE:20721 EMAGE:20721 EMAGE:20721 EMAGE:20721 EMAGE:20721
euxassay_013998_10 euxassay_013998_11 euxassay_013998_12 euxassay_013998_13 euxassay_013998_14
EMAGE:20721 EMAGE:20721 EMAGE:20721 EMAGE:20721 EMAGE:20721
euxassay_013998_15 euxassay_013998_16 euxassay_013998_17 euxassay_013998_18 euxassay_013998_19
EMAGE:20721 EMAGE:20721 EMAGE:20721 EMAGE:20721
euxassay_013998_20 euxassay_013998_21 euxassay_013998_22 euxassay_013998_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20721Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20721_wholemount_strong.wlz
20721_wholemount_moderate.wlz
20721_wholemount_weak.wlz
20721_wholemount_possible.wlz
20721_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20721_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39532
Entity Detected:D2Wsu81e, DNA segment, Chr 2, Wayne State University 81, expressed ( MGI:106544)
Sequence:sense strand is shown

>T39532
ACCTGGAGTGTCCACAGTACCTGAGAAAGGCGTTCTTCCCCAAGCACCAAGATCTGCAGTTTGCAGGAAT
CCTCAACCCCTTGGACAGCCCTCACCACATGCGTCAGGACGAAGAGTCTGAGTTTCGAGAAGGCGTCGTG
GTGGACCGGCCCACCAAGGCAGGCCATGGCTCTTTGGTCAACTGTGGAATGAAGAAGGAGGTGAAGATTG
ACAAGAAACTGGACCCTGGACTTCGGGTGACCGTGCGACTGAACCAGCAGCAGCTCCCAGAATGCAAGAC
ATACAAAGGAACAGTTGTGTCATCACAGGACCCCCGCACCAAAGCTTGTCTCTACTGGGGCTACACTGTC
CGGCTGGCCTCTTGCCTCAGCGCTGTGTTTGCTGAGGCTCCCTTCCAGGATGGGTACGACCTGACCATTG
GGACATCAGAACGTGGCTCCGACGTGGCCTCTGCCCAGCTGCCCAGCTTCAGGCATGCCCTTGTGGTGTT
TGGAGGCCTCCAAGGGCTAGAAGCTGCAGTGGATGCTGACCCGAACCTTGAAGTGGCTGACCCCAGTGTC
CTCTTCGACTTCTATGTCAACACATGCCTTAGCCAGGGCAGCCGTACCATCCGCACCGAGGAGGCCATAC
TCATCTCGCTGGCTGCTCTGCAGCCTGGTCTCACCCAGGTGGGATCGAGGCCCGCCTCCCCGCTTTCTGG
ACCCAGGATGTAGAGGAAGCAGCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 67397. Forward Primer - name:067397_F_cDNA_D2Wsu81e, sequence:ACCTGGAGTGTCCACAGTACCT; Reverse Primer - name:067397_N_SP6_cDNA_D2Wsu81e, sequence:TGCTGCTTCCTCTACATCCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20720 same embryo
 EMAGE:20725 same embryo
 EMAGE:20724 same embryo
 EMAGE:20722 same embryo
 EMAGE:20723 same embryo
 EurExpress:euxassay_013998 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS